Anti-TRPV2 抗体 (ab6183)

Submit a review Q&A (3)References (5)

Key features and details

  • Rabbit polyclonal to TRPV2
  • Reacts with: Rat
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-TRPV2 antibody
    TRPV2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to TRPV2

  • 由来種

    Rabbit

  • 種交差性

    交差種: Rat

  • 免疫原

    Synthetic peptide corresponding to Rat TRPV2 aa 700-800 (C terminal) conjugated to keyhole limpet haemocyanin (Glutaraldehyde).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 特記事項

    VRL 1 is also known as Vanilloid Receptor Like protein-1.

     This product was previously labelled as VRL1

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

ターゲット情報

  • 機能

    Calcium-permeable, non-selective cation channel with an outward rectification. Seems to be regulated, at least in part, by IGF-I, PDGF and neuropeptide head activator. May transduce physical stimuli in mast cells. Activated by temperatures higher than 52 degrees Celsius; is not activated by vanilloids and acidic pH.

  • 配列類似性

    Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV2 sub-subfamily.
    Contains 6 ANK repeats.

  • 翻訳後修飾

    N-glycosylated.
    Phosphorylated by PKA.

  • 細胞内局在

    Cell membrane. Cytoplasm. Melanosome. Translocates from the cytoplasm to the plasma membrane upon ligand stimulation (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • 参照データベース

    • 別名

      • MGC12549 antibody
      • Osm 9 like TRP channel 2 antibody
      • Osm-9-like TRP channel 2 antibody
      • OTRPC2 antibody
      • Sac2b antibody
      • Stretch activated channel 2B antibody
      • Transient receptor potential cation channel subfamily V member 2 antibody
      • TRPV2 antibody
      • TRPV2_HUMAN antibody
      • Vanilloid receptor like protein 1 antibody
      • Vanilloid receptor-like protein 1 antibody
      • VRL 1 antibody
      • VRL antibody
      • VRL-1 antibody
      • VRL1 antibody
      see all

    参考文献 (5)

    ab6183 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab6183 は 5 報の論文で使用されています。

    • Elbaz M  et al. TRPV2 is a novel biomarker and therapeutic target in triple negative breast cancer. Oncotarget 9:33459-33470 (2018). PubMed: 30323891
    • Shimohira D  et al. TRPV2 expression in rat oral mucosa. Histochem Cell Biol 132:423-33 (2009). IHC-FoFr ; Rat . PubMed: 19579031
    • Yamamoto Y  et al. Immunohistochemical colocalization of TREK-1, TREK-2 and TRAAK with TRP channels in the trigeminal ganglion cells. Neurosci Lett 454:129-33 (2009). IHC-Fr ; Rat . PubMed: 19429069
    • Yamamoto Y  et al. Distribution of TRPV1- and TRPV2-immunoreactive afferent nerve endings in rat trachea. J Anat 211:775-83 (2007). PubMed: 17979952
    • Gaudet AD  et al. Regulation of TRPV2 by axotomy in sympathetic, but not sensory neurons. Brain Res 1017:155-62 (2004). PubMed: 15261111

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    Question

    Enclosed please find the western blot result by using ab3243. The exprimental condictions are as follows: 1. Loading total protein 40ug/lane in 9% separation SDS-PAGE gel (The second lane: bladder, as my postive control). 2. transfer to PVDF membrane (Millpore immobilon-FL), 47 Volt for 60 min. 3. blocking: 5% skim milk in TBST (0.05% Tween 20) 4. primary antibody: ab3243 (lot: 237021) by using 0.1ug/ml as final concentration at 4oC overnight. 5. secondary antibody: goat anti-rabbit HRP (Pierce) 1:2500 dilution at room temperature for 1 hour. 6. ECL: Supersignal west Pico Chemiluminescent (Pierce 34080)

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    Answer

    Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I have read your technical questionaire and I have a few comments. We have details of this antibody applied by IHC from customer feedback, but not western blotting. You are obtaining an alarming number of extraneous bands. I would like to recommend that in order to obtain more specific results that you use 3% BSA as a blocking agent. This is routinely employed by our lab team. I would also like to recommend that you increase the percentage of Tween 20 to 0.1% in an effort to address the non-specific nature of this antibody. I would also like to recommend that you perform an overnight block of your membrane using this agent. If this does not improve your results please get back in touch with me and I am happy to provide you with a replacement antibody or credit against your purchase provided that you purchased this antibody within the past 90 days.

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    Question

    What is the concentration?

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    Answer

    Thank you for your enquiry. The concentration of this antibody is 1 mg/ml. Please contact us again if you have any additional questions.

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    Question

    What is the concentration? Abcam Lot# 58240.

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    Answer

    The concentration is 1 mg/ml.

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