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  1. Link

    trpv1-antibody-ab6166.pdf

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Anti-TRPV1 抗体 (ab6166)

  • Datasheet
Reviews (2)Q&A (5)References (14)

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Western blot - Anti-TRPV1 antibody (ab6166)
  • Immunohistochemistry - Free Floating - Anti-TRPV1 antibody (ab6166)
  • Immunohistochemistry - Free Floating - Anti-TRPV1 antibody (ab6166)
  • Immunohistochemistry - Free Floating - Anti-TRPV1 antibody (ab6166)

Key features and details

  • Rabbit polyclonal to TRPV1
  • Suitable for: WB, IHC-FrFl
  • Reacts with: Mouse
  • Isotype: IgG

こちらの製品もご検討ください

タンパク質
Product image
Recombinant Human TRPV1 protein (ab159807)
二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
一次抗体
Product image
Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176)

関連製品

製品の概要

  • 製品名

    Anti-TRPV1 antibody
    TRPV1 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to TRPV1
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: WB, IHC-FrFlmore details
  • 種交差性

    交差種: Mouse
  • 免疫原

    Synthetic peptide corresponding to Rat TRPV1 aa 800-900 (C terminal) conjugated to keyhole limpet haemocyanin (Glutaraldehyde).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 特記事項

    This antibody will stain a selected population of sensory neurons in the dorsal root ganglion. It is unsuitable for staining nerve fibres or terminals.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • 精製度

    Whole antiserum
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • More Ion Channels
    • Neuroscience
    • Sensory System
    • Somatosensory system
    • Nociception
    • Neuroscience
    • Processes

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human TRPV1 protein (ab159807)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab6166の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/1000. Predicted molecular weight: 94.976 kDa.
IHC-FrFl
1/100 - 1/2000.

Use at 1/500– 1/2000 dilution for DAB staining and 1/100 – 1/500 dilution for immunofluorescence staining.

特記事項
WB
1/1000. Predicted molecular weight: 94.976 kDa.
IHC-FrFl
1/100 - 1/2000.

Use at 1/500– 1/2000 dilution for DAB staining and 1/100 – 1/500 dilution for immunofluorescence staining.

ターゲット情報

  • 機能

    Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accubens by affecting AMPA receptors endocytosis.
  • 組織特異性

    Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level).
  • 配列類似性

    Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV1 sub-subfamily.
    Contains 6 ANK repeats.
  • ドメイン

    The association domain (AD) is necessary for self-association.
  • 翻訳後修飾

    Phosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phoshphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PKCM and probably PKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity.
  • 細胞内局在

    Cell junction > synapse > postsynaptic cell membrane. Cell projection > dendritic spine membrane.
  • Target information above from: UniProt accession Q8NER1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 193034 Mouse
    • SwissProt: Q704Y3 Mouse
    • Unigene: 447485 Mouse
    • 別名

      • Capsaicin receptor antibody
      • DKFZp434K0220 antibody
      • osm 9 like TRP channel 1 antibody
      • Osm-9-like TRP channel 1 antibody
      • OTRPC1 antibody
      • Transient receptor potential cation channel subfamily V member 1 antibody
      • TRPV 1 antibody
      • Trpv1 antibody
      • TRPV1_HUMAN antibody
      • Vanilloid receptor 1 antibody
      • Vanilloid receptor subtype 1 antibody
      • VR 1 antibody
      • VR1 antibody
      see all

    画像

    • Western blot - Anti-TRPV1 antibody (ab6166)
      Western blot - Anti-TRPV1 antibody (ab6166)
      Anti-TRPV1 antibody (ab6166) at 1/1000 dilution + Mouse dorsal root ganglia at 20 µg

      Predicted band size: 94.976 kDa
      Observed band size: 75 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry - Free Floating - Anti-TRPV1 antibody (ab6166)
      Immunohistochemistry - Free Floating - Anti-TRPV1 antibody (ab6166)

      Immunohistochemistry analysis of 4% paraformaldehyde fixed/free-floating mouse dorsal root ganglia detecting TRPV1 using ab6166 at a 1/500 dilution. 40x Magnification. Right: Negative control.

    • Immunohistochemistry - Free Floating - Anti-TRPV1 antibody (ab6166)
      Immunohistochemistry - Free Floating - Anti-TRPV1 antibody (ab6166)

      Immunohistochemistry analysis of 4% paraformaldehyde fixed/free-floating mouse dorsal root ganglia detecting TRPV1 using ab6166 at a 1/500 dilution. 40x Magnification. Right: Negative control.

    • Immunohistochemistry - Free Floating - Anti-TRPV1 antibody (ab6166)
      Immunohistochemistry - Free Floating - Anti-TRPV1 antibody (ab6166)

      Immunohistochemistry analysis of 4% paraformaldehyde fixed/free-floating mouse dorsal root ganglia detecting TRPV1 using ab6166 at a 1/500 dilution.

    プロトコール

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet download

      Download

    参考文献 (14)

    ab6166 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab6166 は 14 報の論文で使用されています。

    • Oh S  et al. Radiofrequency Irradiation Modulates TRPV1-Related Burning Sensation in Rosacea. Molecules 26:N/A (2021). PubMed: 33800730
    • Chen Y  et al. Electroacupuncture at ST36 Relieves Visceral Hypersensitivity via the NGF/TrkA/TRPV1 Peripheral Afferent Pathway in a Rodent Model of Post-Inflammation Rectal Hypersensitivity. J Inflamm Res 14:325-339 (2021). PubMed: 33584100
    • Zhang Y  et al. The mechanism of Annexin A1 to modulate TRPV1 and nociception in dorsal root ganglion neurons. Cell Biosci 11:167 (2021). PubMed: 34446102
    • Di Y  et al. Ursolic acid protects against cisplatin-induced ototoxicity by inhibiting oxidative stress and TRPV1-mediated Ca2+-signaling. Int J Mol Med 46:806-816 (2020). PubMed: 32626955
    • Xing F  et al. MZF1 in the Dorsal Root Ganglia Contributes to the Development and Maintenance of Neuropathic Pain via Regulation of TRPV1. Neural Plast 2019:2782417 (2019). PubMed: 31582966
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-7 of 7 Abreviews or Q&A

    Western blot abreview for Anti-TRPV1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (dorsal ganglia)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Specification
    dorsal ganglia
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 22°C
    Read More

    DR. Alexandre Magno

    Verified customer

    投稿 Aug 14 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-TRPV1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Rectum)
    Permeabilization
    Yes - After antigen retrival and a PBS wash (3X), 5% ice cold acetone was applied for 5 minutes, slides were then washed with PBS (3x) and 0.1% Triton was applied for 15 minutes.
    Specification
    Rectum
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Donna Daly

    Verified customer

    投稿 Sep 06 2017

    Question

    thank you so much. We will wainting for a replacement vial of ab6166 in your next shipment.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 21 2012

    Answer

    I'm happy to help! Please let me know if you need anything further.

    Read More

    Abcam Scientific Support

    Answered on Mar 21 2012

    Question

    I confirm that our PO is xxxx (13th of december 2011), and your reference numeber is xxxxx.
    Please let me know is you can send us asap a new vial of ab6166.

    Thanks in advance

    Read More

    Abcam community

    Verified customer

    Asked on Mar 21 2012

    Answer

    Thanks for the additional information.

    I have located the order on our system and have arranged for a replacement vial of ab6166 to be included in Abcam's next shipment to you.

    For your records I have found the Abcam order reference number for that order was xxxx.

    Please let me know if you need anything further.

    Read More

    Abcam Scientific Support

    Answered on Mar 21 2012

    Question

    Dear All,
    please find in attachement a complaint for ab6166.
    Please note that I can't provide you with the lot number because after aliquoting the antibody, the customer threw away the original vial.
    The customer has already used this antibody (another lot, but she doesn't not the lot number), with excellent results.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 20 2012

    Answer

    Thank you for your enquiry.

    I am sorry to hear of your customer's troubles using ab6166. I have read the attached complaint report and it does appear the most recent vial of ab6166 is not working as expected. I would be happy to offer a replacement or credit.

    I have been unsuccessful in locating the order based on the PO. Please double check this number. Thanks!

    Read More

    Abcam Scientific Support

    Answered on Mar 20 2012

    Question

    Dear All,  our customer bought your AB6166 (our PO 3426), but the color of the liquid within the vial is dark red. The lot number is GR21183-1 She has already bought this antibody previously but the color was light yellow. Is it normal? The customer test it and she found a red background in her tissue sections. Please let me know Thanks in advance Kind regards

    Read More

    Abcam community

    Verified customer

    Asked on Jan 19 2012

    Answer

    Thank you for your enquiry and interest. This antibody is not purified and as the datasheet indicates we sell it as whole antiserum. ab6166 is a polyclonal antibody and there is batch-to-batch variation; some bleed may contain higher levels of red blood cells/or breakdown product of hemoglobulins than others. If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Jan 19 2012

    Question

    Is this antibody to the intracellular or extracellular part of the receptor?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 13 2001

    Answer

    This antibody was raised against the intra-cellular C-terminal.

    Read More

    Abcam Scientific Support

    Answered on Feb 15 2001

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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