製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to TRPM7
  • 由来種
    Goat
  • アプリケーション
    適用あり: IHC-P, IHC-Fr, ELISA, ICC/IF, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Crayfish
    交差が予測される動物種: Dog, Pig
  • 免疫原

    Synthetic peptide: TKESESTNSVRLML, corresponding to C terminal amino acids 1852-1865 of Human TRPM7.

  • ポジティブ・コントロール
    • Daudi lysate
  • 特記事項

    In response to customer feedback and in-house testing the latest lot doesn't pass the WB quality testing so we have decided to remove the application for now. Please contact technical@abcam.com for more details.

     

    Please see reference He et al for details of the size of the protein detected by SDS-PAGE. Principal Names: TRPM7; transient receptor potential cation channel, subfamily M, member 7; CHAK; CHAK1; LTRPC7; FLJ20117; TRP-PLIK; LTRPC ion channel family member 7; homolog of mouse transient receptor potential-phospholipase C-interacting kinase CHaK hypothetical protein. Official Gene Symbol - TRPM7 GenBank Accession Number – XP_030709 LocusLink ID - 54822 (human).

     Can be blocked with Human TRPM7 peptide (ab22777).

     

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration. PubMed: 21183474
IHC-Fr 1/1000. PubMed: 16285951
ELISA 1/16000.
ICC/IF Use at an assay dependent concentration. PubMed: 16285951
IHC-FoFr Use at an assay dependent concentration. PubMed: 19734892

ターゲット情報

  • 機能
    Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1).
  • 関連疾患
    Defects in TRPM7 are a cause of susceptibility to amyotrophic lateral sclerosis-parkinsonism/dementia complex type 1 (ALS-PDC1) [MIM:105500]; also called amyotrophic lateral sclerosis-parkinsonism/dementia complex of Guam or Guam disease. Amyotrophic lateral sclerosis-parkinsonism/dementia complex type 1 is a neurodegenerative disorder characterized by chronic, progressive and uniformly fatal amyotrophic lateral sclerosis and parkinsonism-dementia. Both diseases are known to occur in the same kindred, the same sibship and even the same individual.
  • 配列類似性
    In the C-terminal section; belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily.
    In the N-terminal section; belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM7 sub-subfamily.
    Contains 1 alpha-type protein kinase domain.
  • 翻訳後修飾
    Autophosphorylated.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ALSPDC antibody
    • CHAK antibody
    • CHAK1 antibody
    • Channel kinase 1 antibody
    • Channel-kinase 1 antibody
    • Long transient receptor potential channel 7 antibody
    • LTrpC-7 antibody
    • LTrpC7 antibody
    • Transient receptor potential cation channel subfamily M member 7 antibody
    • TRP PLIK antibody
    • TRPM7 antibody
    • TRPM7_HUMAN antibody
    see all

参考文献

This product has been referenced in:
  • Liu A  et al. TRPM7 in CHBP-induced renoprotection upon ischemia reperfusion-related injury. Sci Rep 8:5510 (2018). Read more (PubMed: 29615639) »
  • Gao SL  et al. TRPM7 is overexpressed in bladder cancer and promotes proliferation, migration, invasion and tumor growth. Oncol Rep 38:1967-1976 (2017). Read more (PubMed: 28791418) »
See all 16 Publications for this product

レビューと Q&A

1-10 of 20 Abreviews or Q&A

Answer

Could you send us the following information;

- lot number
- purchase order number

Thanks!

Read More

Question

For your knowledge, I enclose the comments of two reviewers to a paper that we submitted to an international journal. We addressed the role of TRPM7 in endothelial cells and used your anti-TRPM7 antibody Ab729 e 72155 . Our manuscript was rejected because the antibodies recognize a band with a MW around 160 kDa and, although this band disappears after silencing with specific siRNA, this jeopardize the whole work.

I would appreciate your comments.

Best regards

Reviewer 1. The authors use the anti-TRPM7 antibody from Abcam for their experiments. The western blots shown in nearly all the figures identify TRPM7 as a 150-160 kDa band. The molecular weight of the full length TRPM7 protein is roughly 220 KDa. It is possible that the 150 kDa fragment shown in the manuscript is a proteolytic fragment of TRPM7. In support of this view is the fact that knockdown of TRPM7 reduces levels of this band in Figure 4A at 24 hours.

While it is possible that the 150 kDa fragment is from TRPM7, the possibility exists that the 150 kDa band is not in fact TRPM7. The authors need to validate the Abcam antibody by showing that heterologously expressed TRPM7 is recognized by the Abcam antibody. In addition, the authors should show that the 150 kDa band in endothelial cells is recognized by a second TRPM7 that recognizes second epitope (another region on TRPM7). Without this data, the data presented is not convincing”.

Reviewer 2. The antibody used in the study stain a protein band of about 160 kDa, whereas the molecular weight of TRPM7 is 212 kDa. Thus, this is not convincing that western blots actually monitor levels of TRPM7. This is a critical issue because the study relies heavily on Western blot analysis of TRPM7 protein expression.

Read More
Answer

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and would like to investigate the complaint further. Could you send us the publication or the data you have gained with this antibody? Could you also fill the attached questionnaire?

Thank you very much for your cooperation. I will look forward to hearing from you soon.

Read More

Answer

Thank you for your reply.


For anti-TRPM7 the immunogen region for ab729 and ab72155 show 100% homology with the bovine sequence. Therefore, either of these antibodies should work with bovine.

http://www.uniprot.org/uniprot/F1MPF0

If you'd like to test a TRPM7 antibody in bovine, let me know and I can enroll you in our testing discount program we talked about on the phone.

Read More

Answer

Thank you for contacting us.

The ab let me know the following:
The observed molecular weight corresponds to earlier findings in the literature with different antibodies. We are not sure if this applies also to TRPM6.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for contacting us.

I am checking with the lab regarding ab729 and will let you know.

As for TRPM6, which antibody are you refering to?

I look forward to hear back from you.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for your reply. The details provided enable us to closely monitor the quality of our products.

I am sorry these products did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order numbers xxx for ab729 and xxx for ab85016, respectively.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that these free of charge replacement vials are also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Answer

Thank you for your email.

I can definitely send you 2 replacement antibodies to the address you mentioned, but I would need to know which ones you'd like to receive.

I can send the same antibodies and different lots if we have them. I can send other TRPM7 antibodies than the ones you already tried (e.g. ab72155, ab63088). Alternatively, if you need any other antibody to a different target than TRPM7,I can send those too, but would need to know which target protein and antibody you might be looking for.

No, the antibodies you had tried do not need to be returned to us.

I look forward to hear back from you with more information.

Read More

Question
Answer

Thank you for your reply.

I have located both your orders for ab729 and ab85016.

Could you please let me know if you would prefer a free of charge replacement for each of the antibodies, a credit or refund? We do carry other TRPM7 antibodies. Alternatively, you can also choose antibodies to a different target protein.

I look forward to hear back and resolve this case for you.

Read More

Question

Catalog number(s): 85016

Product(s): Mouse monoclonal [S74-25] to TRPM7

Survey results
----------------------------------------------------------------------------------
Telephone section
Q. Did you contact Abcam's Scientific Support by telephone in reference to this complaint?
A. No
Q. Was it clear from the phone options which number you needed to press to get your issue resolved?
A.

Protocol advice section
Q. Did Abcam's Scientific Support give you protocol advice?
A.Yes
Q. How did you feel about being given protocol advice?
A.I requested protocol advice
Q. Did the product work successfully after following the advice?
A.No
Q. Why have you not tried the protocol advice? (Select all that apply.)
A.

Free of charge replacement section
Q. Did Abcam's Scientific Support give you a free of charge replacement?
A. No

Q. Did the replacement product work successfully?
A.
Credit note / refund section
Q. Did Abcam's Scientific Support give you a credit note or a refund?
A.No

Q. Would you have preferred a different outcome from receiving a credit note or refund?
A.
Overall satisfaction section
Q. How satisfied are you with how the complaint was handled?
A.Unsatisfied
Q. Could we have done anything to better resolve the problem?
A.Maybe
Q. Would you mind if our scientific support team contacted you to better understand what happened and reach an agreeable solution?
A.No, I would not mind
Q. Please share with us any final thoughts you may have about Abcam, the complaints process, or our products.
A.I want to a sample working good.

Read More
Answer

Thank you for your recent participation in our survey.
I am very sorry to hear that the protocol tips did not work.

You had used ab729 as well as ab85016.What tips have you tried and have the results changed at all?

Please let me know your order or PO number for these antibodies or the institution or order date so that I can locate your order in our system.

If the products were purchased within last year, I'd be happy to offer you either a free of charge repalcement, a credit or a refund.

I look forward to hear back from you and resolve this for you.

Read More

Answer

Thank you for contacting us. I have received WB protocol information for ab729, but am still waiting for more information regarding ab85016. Thank you for your continued patience in the meantime. For ab729, we have seen a 150-160kDa band in DDI, and in human brain lysates. All samples are boiled in Laemmli sample buffer before loading. The standard WB conditions are as follows: Starting material: We find enhancement of transfer of proteins on PVDF membranes compared to nitrocellulose membranes. • The membrane is blocked in 3% (w/v) skimmed milk in TBS-T for 1 hr at room temperature with agitation (can be blocked overnight at 4°C without agitation). • Primary antibody is incubated in the blocking buffer for 1 hr at room temperature with agitation. • The anti-goat secondary antibody should be used according to manufacturer's recommendations. Ideally, with minimal cross-reactivity with human and rodent serum proteins. It should be diluted in the blocking buffer and incubated for 1 hr at room temperature with agitation. • Wash with TBST three times after primary and after secondary antibody, each wash lasting for 5-10 minutes. • Use ECL-plus rather than ECL, which is more sensitive for detection. The blots are exposed to X-ray film. Tris buffered saline (TBS): 20mM Tris, pH7.4 in 150mM NaCl TBS-T: TBS with 0.05% v/v Tween-20 Important comments: After transfer on PVDF membrane, the quality of the transfer is monitored by staining in Ponceau red. Areas with visible air bubbles are marked with permanent ink and lanes where an air bubble was trapped at the position of bands of interest should be avoided for analysis. The Ponceau is removed by Tween-containing buffer. Primary incubation overnight at 4C requires much further dilution of the primary antibody. Still, non-specific background may be expected when incubating overnight.   I might suggest trying to use RIPA buffer as lysis buffer for your samples, as the buffer is stronger than NP40 and will be able to keep the hydrophobic protein in solution better. Also, adding up to 0.1% SDS to the transfer buffer can help prevent that the protein precipitates in the gel during the transfer. Please also let me know the answers to my questions in my last email as this will be of help to determine the cause of the problem better. I hope this information is helpful to you. I will be in touch regarding ab85016. Please let me know if any of the suggestions are of help. And if not, please let me know your order or PO number so that we can resolve this issue to your satisfaction.

Read More

1-10 of 20 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録