Anti-TRPM7 + TRPM6 抗体 [EPR4582] (ab109438)
![Western blot - Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438)](https://www.abcam.co.jp/ps/products/109/ab109438/Images/ab109438-122283-anti-trpm7-trpm6-antibody-epr4582-western-blot.jpg "Western blot - Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4582] to TRPM7 + TRPM6
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-TRPM7 + TRPM6 antibody [EPR4582] -
製品の詳細
Rabbit monoclonal [EPR4582] to TRPM7 + TRPM6 -
由来種
Rabbit -
特異性
The immunogen used for this product shares 86% homology with TRPM6 (ten amino acid stretch with 100% homology). ab109438 has been shown to cross react with TRPM6 in WB.
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アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- HuT-78, JAR, and HepG2 whole cell lysate (ab7900). HeLa cells and cell lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4582 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109438の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 213 kDa.
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 213 kDa. |
ターゲット情報
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細胞内局在
TRPM7: Membrane. TRPM6: Multi-pass membrane protein. -
参照データベース
- Entrez Gene: 140803 Human
- Entrez Gene: 54822 Human
- Omim: 605692 Human
- Omim: 607009 Human
- SwissProt: Q5VYG5 Human
- SwissProt: Q96LV9 Human
- SwissProt: Q96QT4 Human
- SwissProt: Q9BX84 Human
see all
画像
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Western blot - Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438)All lanes : Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : HuT-78 cell lysate
Lane 3 : JAR cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 213 kDa -
![Western blot - Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438)](https://www.abcam.co.jp/ps/products/109/ab109438/Images/ab109438-324289-anti-trpm7-trpm6-antibody-epr4582-western-blot.jpg)
Western blot - Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438)All lanes : Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438) at 1/500 dilution
Lane 1 : His-Tagged human TRPM6 (aa 1755 to 2022) recombinant protein with 5% NFDM/TBST
Lane 2 : His-Tagged human TRPM7 (aa 1599 to 1986) recombinant protein with 5% NFDM/TBST
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 213 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 3 seconds -
![Western blot - Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438)](https://www.abcam.co.jp/ps/products/109/ab109438/Images/ab109438-298911-anti-trpm7-trpm6-antibody-epr4582-western-blot.jpg)
Western blot - Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438)Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438) at 1/1000 dilution (purified) + JAR (Human placenta choriocarcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 213 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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![Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438)](https://www.abcam.co.jp/ps/products/109/ab109438/Images/ab109438-6-benefits-of-recombinant-antibodies.png)
Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (12)
ab109438 は 12 報の論文で使用されています。
- Zhao Y et al. CD82-TRPM7-Numb signaling mediates age-related cognitive impairment. Geroscience 42:595-611 (2020). PubMed: 32088828
- Chen X & Song D LPS promotes the progression of sepsis by activation of lncRNA HULC/miR-204-5p/TRPM7 network in HUVECs. Biosci Rep 40:N/A (2020). PubMed: 32484206
- Lefebvre T et al. TRPM7/RPSA Complex Regulates Pancreatic Cancer Cell Migration. Front Cell Dev Biol 8:549 (2020). PubMed: 32733880
- Tashiro M et al. TRPM7 silencing attenuates Mg2+ influx in cardiac myoblasts, H9c2 cells. J Physiol Sci 70:47 (2020). PubMed: 33028185
- Liu L et al. TRPM7 promotes the epithelial-mesenchymal transition in ovarian cancer through the calcium-related PI3K / AKT oncogenic signaling. J Exp Clin Cancer Res 38:106 (2019). PubMed: 30819230