Anti-TRPM2 抗体 (ab11168)
Key features and details
- Rabbit polyclonal to TRPM2
- Suitable for: IHC-P, WB, IP
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-TRPM2 antibody
TRPM2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TRPM2 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, IPmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Mouse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant -
免疫原
Synthetic peptide corresponding to Human TRPM2 aa 650-750.
Database link: 7226 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Antibodies were affinity purified using the peptide immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11168の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 1 µg/ml.
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WB |
1/300. Predicted molecular weight: 171 kDa.
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IP |
Use at 1-4 µg/mg of lysate.
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特記事項 |
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IHC-P
Use a concentration of 1 µg/ml. |
WB
1/300. Predicted molecular weight: 171 kDa. |
IP
Use at 1-4 µg/mg of lysate. |
ターゲット情報
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機能
Nonselective, voltage-independent cation channel mediating sodium and calcium ion influx in response to oxidative stress. Extracellular calcium passes through the channel and acts from the intracellular side as a positive regulator in channel activation. Activated by ADP-ribose, nicotinamide adenine dinucleotide (NAD(+)), reactive nitrogen species and arachidonic acid. Inactivated by intracellular ATP. Confers susceptibility to cell death following oxidative stress. Isoform 2 does not seem to be regulated by ADPR. Has ADP-ribose pyrophosphatase activity. -
組織特異性
Highly expressed in brain and peripheral blood cells, such as neutrophils. Also detected in bone marrow, spleen, heart, liver and lung. Isoform 2 is found in neutrophil granulocytes. -
配列類似性
Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM2 sub-subfamily.
Contains 1 nudix hydrolase domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 7226 Human
- Entrez Gene: 28240 Mouse
- Entrez Gene: 294329 Rat
- Omim: 603749 Human
- SwissProt: O94759 Human
- SwissProt: Q5KTC0 Mouse
- SwissProt: Q91YD4 Mouse
- SwissProt: Q5G856 Rat
see all -
別名
- EREG 1 antibody
- EREG1 antibody
- Estrogen responsive element associated gene 1 antibody
see all
画像
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All lanes : Anti-TRPM2 antibody (ab11168) at 0.1 µg
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) membrane preparation at 50 µg
Lane 2 : Jurkat membrane preparation at 15 µg
Lane 3 : Jurkat membrane preparation at 5 µg
Developed using the ECL technique.
Predicted band size: 171 kDa
Exposure time: 3 minutes -
IHC image of ab11168 staining in rat brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11168, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Detection of human TRPM2 by western blot of immunoprecipitates. Samples: Membrane Prep (0.5 or 1.0mg per IP reaction; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-TRPM2 antibody A300-414A (lot A300-414A-3) used for IP at 6ug per reaction. TRPM2 was also immunoprecipitated by rabbit anti-TRPM2 antibody A300-413A. For blotting immunoprecipitated TRPM2, A300-414A was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (20)
ab11168 は 20 報の論文で使用されています。
- Wang Y et al. Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling pathway. Mol Biol Rep 49:7337-7345 (2022). PubMed: 35585377
- Li X et al. The Janus-faced role of TRPM2-S in retroperitoneal liposarcoma via increasing ROS levels. Cell Commun Signal 20:128 (2022). PubMed: 36008839
- Yin YL et al. Citronellal Attenuates Oxidative Stress-Induced Mitochondrial Damage through TRPM2/NHE1 Pathway and Effectively Inhibits Endothelial Dysfunction in Type 2 Diabetes Mellitus. Antioxidants (Basel) 11:N/A (2022). PubMed: 36421426
- Huang J et al. Methamphetamine and HIV-Tat Protein Synergistically Induce Oxidative Stress and Blood-Brain Barrier Damage via Transient Receptor Potential Melastatin 2 Channel. Front Pharmacol 12:619436 (2021). PubMed: 33815104
- Lin R et al. TRPM2 promotes pancreatic cancer by PKC/MAPK pathway. Cell Death Dis 12:585 (2021). PubMed: 34099637