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Anti-TRPM2 抗体 (ab11168)

Reviews (2)Q&A (3)References (16)
Western blot - Anti-TRPM2 antibody (ab11168)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM2 antibody (ab11168)
  • Immunoprecipitation - Anti-TRPM2 antibody (ab11168)

Key features and details

  • Rabbit polyclonal to TRPM2
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Rat, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-TRPM2 antibody
    TRPM2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to TRPM2

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, WB, IPmore details

  • 種交差性

    交差種: Rat, Human
    交差が予測される動物種: Mouse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant

  • 免疫原

    Synthetic peptide corresponding to Human TRPM2 aa 650-750.
    Database link: 7226

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab11168の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use a concentration of 1 µg/ml.
WB
1/300. Predicted molecular weight: 171 kDa.
IP
Use at 1-4 µg/mg of lysate.
特記事項
IHC-P
Use a concentration of 1 µg/ml.
WB
1/300. Predicted molecular weight: 171 kDa.
IP
Use at 1-4 µg/mg of lysate.

ターゲット情報

  • 機能

    Nonselective, voltage-independent cation channel mediating sodium and calcium ion influx in response to oxidative stress. Extracellular calcium passes through the channel and acts from the intracellular side as a positive regulator in channel activation. Activated by ADP-ribose, nicotinamide adenine dinucleotide (NAD(+)), reactive nitrogen species and arachidonic acid. Inactivated by intracellular ATP. Confers susceptibility to cell death following oxidative stress. Isoform 2 does not seem to be regulated by ADPR. Has ADP-ribose pyrophosphatase activity.

  • 組織特異性

    Highly expressed in brain and peripheral blood cells, such as neutrophils. Also detected in bone marrow, spleen, heart, liver and lung. Isoform 2 is found in neutrophil granulocytes.

  • 配列類似性

    Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM2 sub-subfamily.
    Contains 1 nudix hydrolase domain.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • EREG 1 antibody
    • EREG1 antibody
    • Estrogen responsive element associated gene 1 antibody
    • Estrogen responsive element associated gene 1 protein antibody
    • Estrogen-responsive element-associated gene 1 protein antibody
    • KNP 3 antibody
    • KNP3 antibody
    • Long transient receptor potential channel 2 antibody
    • LTRPC 2 antibody
    • LTrpC-2 antibody
    • LTrpC2 antibody
    • MGC133383 antibody
    • NUDT9H antibody
    • NUDT9L1 antibody
    • putative Ca2+ channel protein antibody
    • putative Ca2+ channel protein antibody
    • Transient receptor potential cation channel subfamily M member 2 antibody
    • Transient receptor potential channel 7 antibody
    • TRPC 7 antibody
    • TrpC7 antibody
    • TRPM 2 antibody
    • Trpm2 antibody
    • TRPM2_HUMAN antibody
    see all

画像

  • Western blot - Anti-TRPM2 antibody (ab11168)
    Western blot - Anti-TRPM2 antibody (ab11168)
    All lanes : Anti-TRPM2 antibody (ab11168) at 0.1 µg

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) membrane preparation at 50 µg
    Lane 2 : Jurkat membrane preparation at 15 µg
    Lane 3 : Jurkat membrane preparation at 5 µg

    Developed using the ECL technique.

    Predicted band size: 171 kDa


    Exposure time: 3 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM2 antibody (ab11168)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM2 antibody (ab11168)
    IHC image of ab11168 staining in rat brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11168, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunoprecipitation - Anti-TRPM2 antibody (ab11168)
    Immunoprecipitation - Anti-TRPM2 antibody (ab11168)

    Detection of human TRPM2 by western blot of immunoprecipitates. Samples: Membrane Prep (0.5 or 1.0mg per IP reaction; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-TRPM2 antibody A300-414A (lot A300-414A-3) used for IP at 6ug per reaction. TRPM2 was also immunoprecipitated by rabbit anti-TRPM2 antibody A300-413A. For blotting immunoprecipitated TRPM2, A300-414A was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

参考文献 (16)

ab11168 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab11168 は 16 報の論文で使用されています。

  • Ji M  et al. Cellular channelopathy mediated by hypergravity: IL-6-mediated Nkcc1 activation and enhanced Trpm2 expression in rat atrium. Cell Tissue Res 383:1017-1024 (2021). PubMed: 33159577
  • Huang J  et al. Methamphetamine and HIV-Tat Protein Synergistically Induce Oxidative Stress and Blood-Brain Barrier Damage via Transient Receptor Potential Melastatin 2 Channel. Front Pharmacol 12:619436 (2021). PubMed: 33815104
  • Lin R  et al. TRPM2 promotes pancreatic cancer by PKC/MAPK pathway. Cell Death Dis 12:585 (2021). PubMed: 34099637
  • Hu H  et al. Transient receptor potential melastatin 2 contributes to neuroinflammation and negatively regulates cognitive outcomes in a pilocarpine-induced mouse model of epilepsy. Int Immunopharmacol 87:106824 (2020). PubMed: 32731181
  • Zhao Q  et al. TRPM2 promotes autophagic degradation in vascular smooth muscle cells. Sci Rep 10:20719 (2020). PubMed: 33244095
View all Publications for this product

レビューと Q&A

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1-5 of 5 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-TRPM2 antibody

 Inconclusive
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (cryostat cut,14 micrometer thick sections)
Permeabilization
Yes - 0.1% tritonx-100 in 1x PBS
Specification
cryostat cut,14 micrometer thick sections
Blocking step
Serum as blocking agent for 2 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

Kristina Zuza

Verified customer

投稿 Jun 10 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-TRPM2 antibody

 Inconclusive
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Neutrophil)
Permeabilization
No
Specification
Neutrophil
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Read More

MR. Carl Hobbs

Verified customer

投稿 Sep 25 2015

Question

Thank you for your email. I was away for conference in July and later busy with timelines after the conference. My lab staffs (a Research Associate and a technician) have been doing further testing of the three antibodies we purchased earlier. 1) Anti-TRPM7 (AB85016) antibody: still not able to confirm any 220kDa band of WB in native neuronal cells and HEK cells. 2) TRPM2 antibody (AB11168) is not good as well - still with too many bands on WB using brain tissue. 3) anti-SUR1 antibody (AB32844): using very high concentration (1:100) on WB and long incubation time (3 days), we could see a weak band around 175 kDa. Wondering if the concentration is correct in the lot / shipment? If possible, we would like to have: 1) refund on item #1 (AB85016) and item #2 (AB11168), 2) replacement for anti-SUR1 antibody (AB32844) to try one more time in WB. Thank you very much for your consideration.

Read More
Answer

Thank you for contacting us. I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody. I look forward to receiving your reply. PS: Please include images for each antibody also. Images always helps in understanding the problem quickly.

Read More

Question

The quiry is about both ab11167 and ab11168. Both antibodies are for TRPM2. On the product datasheets, the predicted molecular weight is stated as 17 kDa. Do both antibodies detect TRPM2-L and TRPM-S? Am I right in saying that TRPM2-L has a molecular weight size of 171 kDa and that of TRPM2-S is 95 kDa? Have you done Western blots with these antibodies? Do you have a positive control for them? Do you think you might be able to send me an image of it?

Read More
Answer

Thank you for your enquiry. There appears to be three human isoforms of this target (Swiss Prot). TRPM2-S should have a predicted KDa of 101 and TRPM2-L should have a predicted KDa of 180 (Swiss Prot).I have removed the predicted KDa of 17 from the data sheet as I believe this is a mistake, thank you for bringing this matter to my attention and best of luck with your research.

Read More

Question

The quiry is about both ab11167 and ab11168. Both antibodies are for TRPM2. On the product datasheets, the predicted molecular weight is stated as 17 kDa. Do both antibodies detect TRPM2-L and TRPM-S? Am I right in saying that TRPM2-L has a molecular weight size of 171 kDa and that of TRPM2-S is 95 kDa? Have you done Western blots with these antibodies? Do you have a positive control for them? Do you think you might be able to send me an image of it?

Read More
Answer

Thank you for your enquiry. There appears to be three human isoforms of this target (Swiss Prot). TRPM2-S should have a predicted KDa of 101 and TRPM2-L should have a predicted KDa of 180 (Swiss Prot).I have removed the predicted KDa of 17 from the data sheet as I believe this is a mistake, thank you for bringing this matter to my attention and best of luck with your research.

Read More

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