The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 10 µg/ml.
Use at an assay dependent concentration.
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).Can be blocked with TRPC6 peptide (ab106953).
Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion.
Expressed primarily in placenta, lung, spleen, ovary and small intestine. Expressed in podocytes and is a component of the glomerular slit diaphragm.
Defects in TRPC6 are the cause of focal segmental glomerulosclerosis type 2 (FSGS2) [MIM:603965]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.
Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC6 sub-subfamily. Contains 4 ANK repeats.
Yang X et al. Role of transient receptor potential channel 6 in the odontogenic differentiation of human dental pulp cells. Exp Ther Med14:73-78 (2017).
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Takada H et al. Mechanosensitive ATP release from hemichannels and Ca²? influx through TRPC6 accelerate wound closure in keratinocytes. J Cell Sci127:4159-71 (2014).
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