Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (8%)
Loading amount
7 µg
Specification
Brain
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10µg/mL · Temperature: 23°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit igG
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
No data
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 42 kDa Non-specific: 37 kDa
Positive control
No data
Additional data
Additional Notes
That mark follows precision plus protein standard.
Because we used 8% gel and spent so long time to loading, we lost lower part of mark.
In that image, mark means 250, 150, 100, 75, 50, 37.
And, It looks like protein is next to the mark 50, 37.
Abcam response
We would like to thank the reviewer for submitting this Abreview for this product. As the product had not yet been tested in this application on this tissue type we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this application. The customer has received value off a future purchase for performing these tests. We wish to thank them for participating in our AbTrial program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. KWANGHYUN HUR
Verified customer
投稿 Apr 09 2019