Anti-TRP2/DCT 抗体 (ab74073)
Key features and details
- Rabbit polyclonal to TRP2/DCT
- Suitable for: ELISA, IHC-P, IHC-Fr, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-TRP2/DCT antibody
TRP2/DCT 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TRP2/DCT -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, IHC-P, IHC-Fr, WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human TRP2/DCT (internal sequence).
Database link: P40126 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab74073の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ELISA |
Use at an assay dependent concentration.
|
|
IHC-P | (2) |
Use at an assay dependent concentration.
|
IHC-Fr | (2) |
Use at an assay dependent concentration.
|
WB | (2) |
1/500 - 1/1000. Predicted molecular weight: 59 kDa.
TRP2 protein is a single-pass type I membrane protein, these types of proteins are known to aggregate when boiled, therefore, we recommend heating the lysates in sample buffer for 15-20 minutes at 60-70°C instead of boiling. |
ICC/IF | (2) |
1/100 - 1/500.
|
特記事項 |
---|
ELISA
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Predicted molecular weight: 59 kDa. TRP2 protein is a single-pass type I membrane protein, these types of proteins are known to aggregate when boiled, therefore, we recommend heating the lysates in sample buffer for 15-20 minutes at 60-70°C instead of boiling. |
ICC/IF
1/100 - 1/500. |
ターゲット情報
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機能
Involved in regulating eumelanin and phaeomelanin levels. -
パスウェイ
Pigment biosynthesis; melanin biosynthesis. -
配列類似性
Belongs to the tyrosinase family. -
細胞内局在
Melanosome membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 1638 Human
- Entrez Gene: 13190 Mouse
- Omim: 191275 Human
- SwissProt: P40126 Human
- SwissProt: P29812 Mouse
- Unigene: 301865 Human
- Unigene: 19987 Mouse
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別名
- L dopachrome tautomerase antibody
- DCT antibody
- Dopachrome delta isomerase antibody
see all
画像
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All lanes : Anti-TRP2/DCT antibody (ab74073) at 1/500 dilution
Lane 1 : Extracts from K562 cells with no peptide
Lane 2 : Extracts from K562 cells with peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 59 kDa
Observed band size: 59 kDa
Additional bands at: 117 kDa, 39 kDa. We are unsure as to the identity of these extra bands. -
Immunofluorescence analysis of TRP2/DCT in A549 cells, using ab74073 at a dilution of 1/500.
Left hand panel: no peptide
right hand panel: with immunising peptide. -
ab74073 staining TRP2/DCT in Mouse melan-ink melanocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 5% serum for 30 hours at room temperature. Samples were incubated with primary antibody (1/100 in PBS -T+ 1% BSA) for 12 hours. An undiluted Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (51)
ab74073 は 51 報の論文で使用されています。
- Wohlfeil SA et al. Angiogenic and molecular diversity determine hepatic melanoma metastasis and response to anti-angiogenic treatment. J Transl Med 20:62 (2022). PubMed: 35109875
- Hu SH et al. sPmel17 Secreted by Ultraviolet B-Exposed Melanocytes Alters the Intercellular Adhesion of Keratinocytes. Oxid Med Cell Longev 2022:1856830 (2022). PubMed: 35186181
- Tanwar J et al. Mitofusin-2 Negatively Regulates Melanogenesis by Modulating Mitochondrial ROS Generation. Cells 11:N/A (2022). PubMed: 35203350
- Bonnamour G et al. NR2F1 regulates a Schwann cell precursor-vs-melanocyte cell fate switch in a mouse model of Waardenburg syndrome type IV. Pigment Cell Melanoma Res 35:506-516 (2022). PubMed: 35816394
- Morante V et al. Integrase-Defective Lentiviral Vector Is an Efficient Vaccine Platform for Cancer Immunotherapy. Viruses 13:N/A (2021). PubMed: 33672349