Anti-TRP1 抗体 [TA99] (ab3312)

Reviews (8)Q&A (5)References (24)
Western blot - Anti-TRP1 antibody [TA99] (ab3312)

    Key features and details

    • Mouse monoclonal [TA99] to TRP1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG2a

    製品の概要

    • 製品名

      Anti-TRP1 antibody [TA99]
      TRP1 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [TA99] to TRP1

    • 由来種

      Mouse

    • 特異性

      ab3312 is specific for melanomas and does not react with carcinomas and sarcomas.

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human

    • 免疫原

      SK-MEL-23 Melanoma cell line.

    • ポジティブ･コントロール

      • In Western Blot, this antibody gave a positive signal in human skin tissue lysate.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab3312の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    Use a concentration of 5 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
    特記事項
    WB
    Use a concentration of 5 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).

    ターゲット情報

    • 機能

      Oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid. May regulate or influence the type of melanin synthesized.

    • 組織特異性

      Pigment cells.

    • パスウェイ

      Pigment biosynthesis; melanin biosynthesis.

    • 関連疾患

      Defects in TYRP1 are the cause of albinism oculocutaneous type 3 (OCA3) [MIM:203290]; also known as Rufous oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. Tyrosinase activity is normal and patients have only moderate reduction of pigment. The eyes present red reflex on transillumination of the iris, dilution of color of iris, nystagmus and strabismus. Darker-skinned individuals have bright copper-red coloration of the skin and hair.

    • 配列類似性

      Belongs to the tyrosinase family.

    • 細胞内局在

      Melanosome membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • 5 antibody
        • 5,6 dihydroxyindole 2 carboxylic acid oxidase antibody
        • 6-dihydroxyindole-2-carboxylic acid oxidase antibody
        • b-PROTEIN antibody
        • CAS2 antibody
        • Catalase B antibody
        • CATB antibody
        • DHICA oxidase antibody
        • Glycoprotein 75 antibody
        • GP75 antibody
        • Melanoma antigen gp75 antibody
        • OCA3 antibody
        • TRP antibody
        • TRP-1 antibody
        • TRP1 antibody
        • Tyrosinase related protein 1 antibody
        • Tyrosinase-related protein 1 antibody
        • TYRP antibody
        • TYRP1 antibody
        • TYRP1_HUMAN antibody
        • TYRRP antibody
        see all

      画像

      • Western blot - Anti-TRP1 antibody [TA99] (ab3312)
        Western blot - Anti-TRP1 antibody [TA99] (ab3312)
        Anti-TRP1 antibody [TA99] (ab3312) at 5 µg/ml + Human skin tissue lysate - total protein (ab30166) at 20 µg

        Secondary
        Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 72 kDa
        Observed band size: 72 kDa
        Additional bands at: 61 kDa. We are unsure as to the identity of these extra bands.


        Exposure time: 20 minutes

      参考文献 (24)

      ab3312 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab3312 は 24 報の論文で使用されています。

      • Belote RL & Simon SM Ca2+ transients in melanocyte dendrites and dendritic spine-like structures evoked by cell-to-cell signaling. J Cell Biol 219:N/A (2020). PubMed: 31821412
      • Bissig C  et al. The PIKfyve complex regulates the early melanosome homeostasis required for physiological amyloid formation. J Cell Sci 132:N/A (2019). PubMed: 30709920
      • Chatatikun M  et al. Anti melanogenic effect of Croton roxburghii and Croton sublyratus leaves in a-MSH stimulated B16F10?cells. J Tradit Complement Med 9:66-72 (2019). PubMed: 30671368
      • Pyo JJ  et al. Keratinocyte-derived IL-36? plays a role in hydroquinone-induced chemical leukoderma through inhibition of melanogenesis in human epidermal melanocytes. Arch Toxicol 93:2307-2320 (2019). PubMed: 31256213
      • Bergam P  et al. ABCB6 Resides in Melanosomes and Regulates Early Steps of Melanogenesis Required for PMEL Amyloid Matrix Formation. J Mol Biol N/A:N/A (2018). PubMed: 29940187
      View all Publications for this product

      レビューと Q&A

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      レビューを送る 質問を送る

      1-10 of 13 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-TRP1 antibody [TA99]

       Excellent
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (Melanocytes)
      Permeabilization
      No
      Specification
      Melanocytes
      Blocking step
      BSA as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
      Fixative
      Acetone
      Read More

      Abcam user community

      Verified customer

      投稿 Apr 08 2021

      Immunohistochemistry (Frozen sections) abreview for Anti-TRP1 antibody [TA99]

       Good
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Human Tissue sections (Skin - nevus)
      Permeabilization
      No
      Specification
      Skin - nevus
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
      Fixative
      Acetone
      Read More

      Abcam user community

      Verified customer

      投稿 Apr 21 2020

      Immunocytochemistry/ Immunofluorescence abreview for Anti-TRP1 antibody [TA99]

       Good
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (Melanoma cell line (SK-Mel 28))
      Permeabilization
      No
      Specification
      Melanoma cell line (SK-Mel 28)
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
      Fixative
      Acetone
      Read More

      Abcam user community

      Verified customer

      投稿 Apr 21 2020

      Immunocytochemistry abreview for Anti-TRP1 antibody [TA99]

       Excellent
      Abreviews
      abreview image
      Application
      Immunocytochemistry
      Sample
      Human Cultured Cells (Melanoma cells)
      Permeabilization
      No
      Specification
      Melanoma cells
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
      Fixative
      Acetone
      Read More

      Herr Dr. Christian Ostalecki

      Verified customer

      投稿 May 24 2018

      ELISA abreview for Anti-TRP1 antibody [TA99]

       Inconclusive
      Abreviews
      abreview image
      Application
      ELISA
      Sample
      Human Serum (melanoma patient serum)
      Specification
      melanoma patient serum
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Type
      Direct
      Read More

      Frau. Mirjam Fässler

      Verified customer

      投稿 Sep 12 2017

      Immunohistochemistry (Frozen sections) abreview for Anti-TRP1 antibody [TA99]

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Blocking step
      0.1% triton, 2.5% goat serum, 2% BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 2% · Temperature: 25°C
      Sample
      Human Tissue sections (Human Skin)
      Specification
      Human Skin
      Permeabilization
      Yes - 0.1% tritonx-100
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      投稿 May 09 2014

      Question

      Dear Madam, Sir,

      For in vivo use we need to provide information on possible presence of murine viruses in TA99 Ab. Could you let me know if a test on murine viruses is done and what the results are.

      Regards,

      Read More
      Answer

      Thank you for contacting us and your interest in our products.

      We have not used the anti-TRP1 antibody [TA99] ab3312 in in vivo assays and would therefore not be able to guarantee the results. I would like to point out that the antibody has 0.1% azide as a preservative which is likely to be detrimental due to its toxicity. Ab80781 is a BSA and azide free preparation of the clone TA99.

      We have not confirmed if the preparation of ab3312 or ab80781 does contain any viruses. We do not expect it would but have not confirmed this.

      I hope this information has been of help. If I can be of any further assistance, please do not hesitate to let me know.

      Read More

      Immunocytochemistry/ Immunofluorescence abreview for Anti-TRP1 antibody [TA99]

       Good
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (SK-MEL-28)
      Specification
      SK-MEL-28
      Fixative
      Paraformaldehyde
      Permeabilization
      Yes - 0.5% Triton-X in PBS
      Read More

      Abcam user community

      Verified customer

      投稿 Oct 18 2012

      Question

      Thanks for your kindly reply, after I contacted with this customer, he indicated he would like to change a free of replacement with ab34165 (Anti-Melanoma Associated Antigen 100+ / 7 kDa antibody [NKI/beteb]), could you please help this customer to add this antibody in our next order list?

      Thanks for your kindly help

      Best regards

      Read More
      Answer

      Thank you for confirming these details.

      We have added a free of charge unit of ab34165 to your order xx. This free of charge unit has been provided to replace the ab3312.

      Please wish the customer all the best of success with his research.

      Thank you for your collaboration. Please do not hesitate to contact us again, should you or the customer have any question.

      Read More

      Question

      Dear technical support team:

      This customer has purchased ab3312 (Anti-TRP1 antibody [TA99]) and has conducted the wb several times with human sample. The results show wrong band size, therefore, he wants to ask for your help to modify his experiment step, could you please offer any suggestion to help him?

      I also attached his image in this letter and his experiment step as follow:

      1. Order details:
      Abcam product code: ab3312
      Antibody storage conditions (temperature/reconstitution etc): +4℃

      2. Please describe the problem (high background, wrong band size, more bands, no band etc).
      Wrong band size

      3. On what material are you testing the antibody in WB?
      Species: human
      What's cell line or tissue: RPMI 7951 cell line
      Cell extract or Nuclear extract: Cell extract
      Purified protein or Recombinant protein: total protein

      3. The lysate
      How much protein was loaded: 25ug
      What lysis buffer was used:Tris 50mM,NaCl 150mM,EDTA 10mM,PMSF 1mM,SDS 0.1%,NP40 1%,triton 0.3% in Q-water
      What protease inhibitors were used: NO
      What loading buffer was used: 5X samplebuffer: 4ml 1.5M Tris-Cl pH6.8,10ml glycerol, 5ml beta-mercaptoethanol, 2g SDS, 1ml 1% bromophenol blue
      Phosphatase inhibitors :NO
      Did you heat the samples: temperature and time:95℃,10min

      4. Electrophoresis/Gel conditions/ Transfer conditions
      Reducing or non reducing gel: non reducing gel
      Reducing agent: NO
      Gel percentage : 10%
      Transfer conditions: (Type of membrane, Protein transfer verified): PVDF,

      5. Blocking conditions
      Buffer: TBST
      Blocking agent: milk, BSA, serum, what percentage: milk 5%
      Incubation time:1 h
      Incubation temperature: room temperature

      6. Primary Antibody
      Species: mouse
      Reacts against:
      At what dilution(s) have you tested this antibody:1:1000,1:2000
      What dilution buffer was used: TBST
      Incubation time: over night
      Incubation temperature: 4℃
      What washing steps were done: 5min X3

      7. Secondary Antibody
      Species: goat
      Reacts against:
      At what dilution(s) have you tested this antibody: 1:1000,1:3000
      Incubation time: 1 H
      Wash steps: 5min X3
      Fluorochrome or enzyme conjugate: HRP
      Do you know whether the problems you are experiencing come from the secondary?

      8. Detection method
      ECl, ECl+, other detection method: ECL

      9. Did you apply positive and negative controls along with the samples? Please specify.
      Negative control: H1299, JEG, C9 (human ovarian teratoma transformed epidermal carcinoma cell line)
      C9 reference: http://www.ncbi.nlm.nih.gov/pubmed/11087069Lu MH, et al. Proc Natl Sci Counc Repub China B. 2000:169-77

      10. Optimization attempts
      How many times have you tried the Western? 3 times
      Have you eliminated the possibility that any background bands could be due to the secondary antibody? (Run a “No primary” control): background clear
      Do you obtain the same results every time e.g. are background bands always in the same place? YES
      What steps have you altered? Alter Primary Antibody and Secondary Antibody dilution

      Could you please help this customer to solve the problem?

      Thanks for your kindly help

      Best regards

      Read More
      Answer

      Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

      The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

      Having reviewed the protocol details, I believe this product should have given satisfactory results. It appears that you may have received a faulty vial.

      The only recommandation I can make for a future test, are to use fresh samples with proteinase inhibitors (to exclude all possibility of protein degradation).

      I apologize for the inconvenience and am pleased to offer you a free of charge replacement, or credit note in compensation.

      Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

      Read More

      1-10 of 13 Abreviews or Q&A

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