Key features and details
- Mouse monoclonal [TA99] to TRP1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2a
製品名Anti-TRP1 antibody [TA99]
TRP1 一次抗体 製品一覧
製品の詳細Mouse monoclonal [TA99] to TRP1
特異性ab3312 is specific for melanomas and does not react with carcinomas and sarcomas.
アプリケーション適用あり: WBmore details
SK-MEL-23 Melanoma cell line.
- In Western Blot, this antibody gave a positive signal in human skin tissue lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.1% Sodium azide
Concentration information loading...
精製度Protein G purified
Our Abpromise guarantee covers the use of ab3312 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 5 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).|
機能Oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid. May regulate or influence the type of melanin synthesized.
パスウェイPigment biosynthesis; melanin biosynthesis.
関連疾患Defects in TYRP1 are the cause of albinism oculocutaneous type 3 (OCA3) [MIM:203290]; also known as Rufous oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. Tyrosinase activity is normal and patients have only moderate reduction of pigment. The eyes present red reflex on transillumination of the iris, dilution of color of iris, nystagmus and strabismus. Darker-skinned individuals have bright copper-red coloration of the skin and hair.
配列類似性Belongs to the tyrosinase family.
- Information by UniProt
- 5 antibody
- 5,6 dihydroxyindole 2 carboxylic acid oxidase antibody
- 6-dihydroxyindole-2-carboxylic acid oxidase antibody
Anti-TRP1 antibody [TA99] (ab3312) at 5 µg/ml + Human skin tissue lysate - total protein (ab30166) at 20 µg
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 72 kDa
Observed band size: 72 kDa
Additional bands at: 61 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
ab3312 は 23 報の論文で使用されています。
- Belote RL & Simon SM Ca2+ transients in melanocyte dendrites and dendritic spine-like structures evoked by cell-to-cell signaling. J Cell Biol 219:N/A (2020). PubMed: 31821412
- Chatatikun M et al. Anti melanogenic effect of Croton roxburghii and Croton sublyratus leaves in a-MSH stimulated B16F10?cells. J Tradit Complement Med 9:66-72 (2019). PubMed: 30671368
- Pyo JJ et al. Keratinocyte-derived IL-36? plays a role in hydroquinone-induced chemical leukoderma through inhibition of melanogenesis in human epidermal melanocytes. Arch Toxicol 93:2307-2320 (2019). PubMed: 31256213
- Bergam P et al. ABCB6 Resides in Melanosomes and Regulates Early Steps of Melanogenesis Required for PMEL Amyloid Matrix Formation. J Mol Biol N/A:N/A (2018). PubMed: 29940187
- Kwon SJ et al. Synergistic effect of rice bran extract and extremely low-frequency electromagnetic fields on dermal papilla/melanocytes in melanogenesis. Bioelectromagnetics 39:595-603 (2018). PubMed: 30371954