Anti-TRP1 抗体 [TA99] (ab3312)
![Western blot - Anti-TRP1 antibody [TA99] (ab3312)](https://www.abcam.co.jp/ps/products/3/ab3312/Images/ab3312-7437-anti-trp1-antibody-ta99-western-blot.jpg "Western blot - Anti-TRP1 antibody [TA99] (ab3312)")
Key features and details
- Mouse monoclonal [TA99] to TRP1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2a
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-TRP1 antibody [TA99]
TRP1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [TA99] to TRP1 -
由来種
Mouse -
特異性
ab3312 is specific for melanomas and does not react with carcinomas and sarcomas. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
SK-MEL-23 Melanoma cell line.
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ポジティブ･コントロール
- In Western Blot, this antibody gave a positive signal in human skin tissue lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
TA99 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3312の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 5 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
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| 特記事項 |
|---|
|
WB
Use a concentration of 5 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa). |
ターゲット情報
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機能
Oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid. May regulate or influence the type of melanin synthesized. -
組織特異性
Pigment cells. -
パスウェイ
Pigment biosynthesis; melanin biosynthesis. -
関連疾患
Defects in TYRP1 are the cause of albinism oculocutaneous type 3 (OCA3) [MIM:203290]; also known as Rufous oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. Tyrosinase activity is normal and patients have only moderate reduction of pigment. The eyes present red reflex on transillumination of the iris, dilution of color of iris, nystagmus and strabismus. Darker-skinned individuals have bright copper-red coloration of the skin and hair. -
配列類似性
Belongs to the tyrosinase family. -
細胞内局在
Melanosome membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 7306 Human
- Omim: 115501 Human
- SwissProt: P17643 Human
- Unigene: 270279 Human
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別名
- 5 antibody
- 5,6 dihydroxyindole 2 carboxylic acid oxidase antibody
- 6-dihydroxyindole-2-carboxylic acid oxidase antibody
see all
画像
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Western blot - Anti-TRP1 antibody [TA99] (ab3312)Anti-TRP1 antibody [TA99] (ab3312) at 5 µg/ml + Human skin tissue lysate - total protein (ab30166) at 20 µg
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 72 kDa
Observed band size: 72 kDa
Additional bands at: 61 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (27)
ab3312 は 27 報の論文で使用されています。
- Aktary Z et al. A role for Dynlt3 in melanosome movement, distribution, acidity and transfer. Commun Biol 4:423 (2021). PubMed: 33772156
- Lv J et al. Protoporphyrin IX Stimulates Melanogenesis, Melanocyte Dendricity, and Melanosome Transport Through the cGMP/PKG Pathway. Front Pharmacol 11:569368 (2020). PubMed: 33013408
- Rodboon T et al. Modified Riceberry rice extract suppresses melanogenesis-associated cell differentiation through tyrosinase-mediated MITF downregulation on B16 cells and in vivo zebrafish embryos. Res Pharm Sci 15:491-502 (2020). PubMed: 33628291
- Belote RL & Simon SM Ca2+ transients in melanocyte dendrites and dendritic spine-like structures evoked by cell-to-cell signaling. J Cell Biol 219:N/A (2020). PubMed: 31821412
- Bissig C et al. The PIKfyve complex regulates the early melanosome homeostasis required for physiological amyloid formation. J Cell Sci 132:N/A (2019). PubMed: 30709920