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    trp1-antibody-ta99-ab3312.pdf

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Anti-TRP1 抗体 [TA99] (ab3312)

  • Datasheet
  • SDS
Reviews (10)Q&A (5)References (29)

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Abpromise

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Western blot - Anti-TRP1 antibody [TA99] (ab3312)

    Key features and details

    • Mouse monoclonal [TA99] to TRP1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG2a

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-TRP1 antibody [EPR13063] (ab178676)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産

    製品の概要

    • 製品名

      Anti-TRP1 antibody [TA99]
      TRP1 一次抗体 製品一覧
    • 製品の詳細

      Mouse monoclonal [TA99] to TRP1
    • 由来種

      Mouse
    • 特異性

      ab3312 is specific for melanomas and does not react with carcinomas and sarcomas.
    • アプリケーション

      適用あり: WBmore details
    • 種交差性

      交差種: Human
    • 免疫原

      SK-MEL-23 Melanoma cell line.

    • ポジティブ・コントロール

      • In Western Blot, this antibody gave a positive signal in human skin tissue lysate.
    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • バッファー

      pH: 7.40
      Preservative: 0.1% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • 精製度

      Protein G purified
    • ポリ/モノ

      モノクローナル
    • クローン名

      TA99
    • アイソタイプ

      IgG2a
    • 研究分野

      • Tags & Cell Markers
      • Cell Type Markers
      • Tumor Associated
      • Signal Transduction
      • Metabolism
      • Amino Acids
      • Tags & Cell Markers
      • Cell Type Markers
      • Other Cell Types
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Cancer susceptibility
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      • Cancer
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      • Cancer
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      • Tumor antigens

    関連製品

    • Alternative Versions

      • Anti-TRP1 antibody [TA99] (ab190709)
      • Anti-TRP1 antibody [TA99] - BSA and Azide free (ab212922)
    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
    • Recombinant Protein

      • Recombinant Human TRP1 protein (His tag) (ab219285)

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab3312の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB (1)
    Use a concentration of 5 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
    特記事項
    WB
    Use a concentration of 5 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).

    ターゲット情報

    • 機能

      Oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid. May regulate or influence the type of melanin synthesized.
    • 組織特異性

      Pigment cells.
    • パスウェイ

      Pigment biosynthesis; melanin biosynthesis.
    • 関連疾患

      Defects in TYRP1 are the cause of albinism oculocutaneous type 3 (OCA3) [MIM:203290]; also known as Rufous oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. Tyrosinase activity is normal and patients have only moderate reduction of pigment. The eyes present red reflex on transillumination of the iris, dilution of color of iris, nystagmus and strabismus. Darker-skinned individuals have bright copper-red coloration of the skin and hair.
    • 配列類似性

      Belongs to the tyrosinase family.
    • 細胞内局在

      Melanosome membrane.
    • Target information above from: UniProt accession P17643 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 7306 Human
      • Omim: 115501 Human
      • SwissProt: P17643 Human
      • Unigene: 270279 Human
      • 別名

        • 5 antibody
        • 5,6 dihydroxyindole 2 carboxylic acid oxidase antibody
        • 6-dihydroxyindole-2-carboxylic acid oxidase antibody
        • b-PROTEIN antibody
        • CAS2 antibody
        • Catalase B antibody
        • CATB antibody
        • DHICA oxidase antibody
        • Glycoprotein 75 antibody
        • GP75 antibody
        • Melanoma antigen gp75 antibody
        • OCA3 antibody
        • TRP antibody
        • TRP-1 antibody
        • TRP1 antibody
        • Tyrosinase related protein 1 antibody
        • Tyrosinase-related protein 1 antibody
        • TYRP antibody
        • TYRP1 antibody
        • TYRP1_HUMAN antibody
        • TYRRP antibody
        see all

      画像

      • Western blot - Anti-TRP1 antibody [TA99] (ab3312)
        Western blot - Anti-TRP1 antibody [TA99] (ab3312)
        Anti-TRP1 antibody [TA99] (ab3312) at 5 µg/ml + Human skin tissue lysate - total protein (ab30166) at 20 µg

        Secondary
        Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 72 kDa
        Observed band size: 72 kDa
        Additional bands at: 61 kDa. We are unsure as to the identity of these extra bands.


        Exposure time: 20 minutes

      プロトコール

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      データシートおよび資料

      • SDS download

      • Datasheet download

        Download

      参考文献 (29)

      ab3312 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab3312 は 29 報の論文で使用されています。

      • Aktary Z  et al. A role for Dynlt3 in melanosome movement, distribution, acidity and transfer. Commun Biol 4:423 (2021). PubMed: 33772156
      • Guo D  et al. RAB27A/Melanophilin Blocker Inhibits Melanoma Cell Motility and Invasion. J Invest Dermatol 140:1470-1473.e3 (2020). PubMed: 31978414
      • Li B  et al. Exploring the potential effect and mechanisms of protocatechuic acid on human hair follicle melanocytes. Acta Pharm 70:539-549 (2020). PubMed: 32412435
      • Belote RL & Simon SM Ca2+ transients in melanocyte dendrites and dendritic spine-like structures evoked by cell-to-cell signaling. J Cell Biol 219:N/A (2020). PubMed: 31821412
      • Lv J  et al. Protoporphyrin IX Stimulates Melanogenesis, Melanocyte Dendricity, and Melanosome Transport Through the cGMP/PKG Pathway. Front Pharmacol 11:569368 (2020). PubMed: 33013408
      View all Publications for this product

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-10 of 15 Abreviews or Q&A

      Western blot abreview for Anti-TRP1 antibody [TA99]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Melanoma cells)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      20 µg
      Specification
      Melanoma cells
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Aug 25 2021

      Immunohistochemistry (Frozen sections) abreview for Anti-TRP1 antibody [TA99]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Human Tissue sections (Skin)
      Permeabilization
      No
      Specification
      Skin
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 22°C
      Fixative
      Acetone
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 May 27 2021

      Immunocytochemistry/ Immunofluorescence abreview for Anti-TRP1 antibody [TA99]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (Melanocytes)
      Permeabilization
      No
      Specification
      Melanocytes
      Blocking step
      BSA as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
      Fixative
      Acetone
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Apr 08 2021

      Immunohistochemistry (Frozen sections) abreview for Anti-TRP1 antibody [TA99]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Human Tissue sections (Skin - nevus)
      Permeabilization
      No
      Specification
      Skin - nevus
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
      Fixative
      Acetone
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Apr 21 2020

      Immunocytochemistry/ Immunofluorescence abreview for Anti-TRP1 antibody [TA99]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (Melanoma cell line (SK-Mel 28))
      Permeabilization
      No
      Specification
      Melanoma cell line (SK-Mel 28)
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
      Fixative
      Acetone
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Apr 21 2020

      Immunocytochemistry abreview for Anti-TRP1 antibody [TA99]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry
      Sample
      Human Cultured Cells (Melanoma cells)
      Permeabilization
      No
      Specification
      Melanoma cells
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
      Fixative
      Acetone
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      HERR DR. Christian Ostalecki

      Verified customer

      投稿 May 24 2018

      ELISA abreview for Anti-TRP1 antibody [TA99]

      Inconclusive
      Abreviews
      Abreviews
      abreview image
      Application
      ELISA
      Sample
      Human Serum (melanoma patient serum)
      Specification
      melanoma patient serum
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Type
      Direct
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Frau. Mirjam Fässler

      Verified customer

      投稿 Sep 12 2017

      Immunohistochemistry (Frozen sections) abreview for Anti-TRP1 antibody [TA99]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Blocking step
      0.1% triton, 2.5% goat serum, 2% BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 2% · Temperature: 25°C
      Sample
      Human Tissue sections (Human Skin)
      Specification
      Human Skin
      Permeabilization
      Yes - 0.1% tritonx-100
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 May 09 2014

      Immunocytochemistry/ Immunofluorescence abreview for Anti-TRP1 antibody [TA99]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (SK-MEL-28)
      Specification
      SK-MEL-28
      Fixative
      Paraformaldehyde
      Permeabilization
      Yes - 0.5% Triton-X in PBS
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Oct 18 2012

      Question

      Dear Madam, Sir,

      For in vivo use we need to provide information on possible presence of murine viruses in TA99 Ab. Could you let me know if a test on murine viruses is done and what the results are.

      Regards,

      Read More

      Abcam community

      Verified customer

      Asked on Dec 18 2012

      Answer

      Thank you for contacting us and your interest in our products.

      We have not used the anti-TRP1 antibody [TA99] ab3312 in in vivo assays and would therefore not be able to guarantee the results. I would like to point out that the antibody has 0.1% azide as a preservative which is likely to be detrimental due to its toxicity. Ab80781 is a BSA and azide free preparation of the clone TA99.

      We have not confirmed if the preparation of ab3312 or ab80781 does contain any viruses. We do not expect it would but have not confirmed this.

      I hope this information has been of help. If I can be of any further assistance, please do not hesitate to let me know.

      Read More

      Abcam Scientific Support

      Answered on Dec 18 2012

      1-10 of 15 Abreviews or Q&A

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