Anti-TRP1 抗体 [TA99] (ab3312)

Reviews (7)Q&A (5)References (22)

Key features and details

  • Mouse monoclonal [TA99] to TRP1
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-TRP1 antibody [TA99]
    TRP1 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [TA99] to TRP1

  • 由来種

    Mouse

  • 特異性

    ab3312 is specific for melanomas and does not react with carcinomas and sarcomas.

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    SK-MEL-23 Melanoma cell line.

  • ポジティブ･コントロール

    • In Western Blot, this antibody gave a positive signal in human skin tissue lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 5 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).

ターゲット情報

  • 機能

    Oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid. May regulate or influence the type of melanin synthesized.

  • 組織特異性

    Pigment cells.

  • パスウェイ

    Pigment biosynthesis; melanin biosynthesis.

  • 関連疾患

    Defects in TYRP1 are the cause of albinism oculocutaneous type 3 (OCA3) [MIM:203290]; also known as Rufous oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. Tyrosinase activity is normal and patients have only moderate reduction of pigment. The eyes present red reflex on transillumination of the iris, dilution of color of iris, nystagmus and strabismus. Darker-skinned individuals have bright copper-red coloration of the skin and hair.

  • 配列類似性

    Belongs to the tyrosinase family.

  • 細胞内局在

    Melanosome membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 5 antibody
      • 5,6 dihydroxyindole 2 carboxylic acid oxidase antibody
      • 6-dihydroxyindole-2-carboxylic acid oxidase antibody
      • b-PROTEIN antibody
      • CAS2 antibody
      • Catalase B antibody
      • CATB antibody
      • DHICA oxidase antibody
      • Glycoprotein 75 antibody
      • GP75 antibody
      • Melanoma antigen gp75 antibody
      • OCA3 antibody
      • TRP antibody
      • TRP-1 antibody
      • TRP1 antibody
      • Tyrosinase related protein 1 antibody
      • Tyrosinase-related protein 1 antibody
      • TYRP antibody
      • TYRP1 antibody
      • TYRP1_HUMAN antibody
      • TYRRP antibody
      see all

    画像

    • Western blot - Anti-TRP1 antibody [TA99] (ab3312)
      Western blot - Anti-TRP1 antibody [TA99] (ab3312)
      Anti-TRP1 antibody [TA99] (ab3312) at 5 µg/ml + Human skin tissue lysate - total protein (ab30166) at 20 µg

      Secondary
      Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 72 kDa
      Observed band size: 72 kDa
      Additional bands at: 61 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 20 minutes

    参考文献 (22)

    ab3312 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab3312 は 22 報の論文で使用されています。

    • Chatatikun M  et al. Anti melanogenic effect of Croton roxburghii and Croton sublyratus leaves in a-MSH stimulated B16F10?cells. J Tradit Complement Med 9:66-72 (2019). PubMed: 30671368
    • Pyo JJ  et al. Keratinocyte-derived IL-36? plays a role in hydroquinone-induced chemical leukoderma through inhibition of melanogenesis in human epidermal melanocytes. Arch Toxicol 93:2307-2320 (2019). PubMed: 31256213
    • Bergam P  et al. ABCB6 Resides in Melanosomes and Regulates Early Steps of Melanogenesis Required for PMEL Amyloid Matrix Formation. J Mol Biol N/A:N/A (2018). PubMed: 29940187
    • Kwon SJ  et al. Synergistic effect of rice bran extract and extremely low-frequency electromagnetic fields on dermal papilla/melanocytes in melanogenesis. Bioelectromagnetics 39:595-603 (2018). PubMed: 30371954
    • Wang Q  et al. Involvement of the central hypothalamic-pituitary-adrenal axis in hair growth and melanogenesis among different mouse strains. PLoS One 13:e0202955 (2018). PubMed: 30356231
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 12 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-TRP1 antibody [TA99]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Skin - nevus)
    Permeabilization
    No
    Specification
    Skin - nevus
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 21 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TRP1 antibody [TA99]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Melanoma cell line (SK-Mel 28))
    Permeabilization
    No
    Specification
    Melanoma cell line (SK-Mel 28)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 21 2020

    Immunocytochemistry abreview for Anti-TRP1 antibody [TA99]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (Melanoma cells)
    Permeabilization
    No
    Specification
    Melanoma cells
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Acetone
    Read More

    Herr Dr. Christian Ostalecki

    Verified customer

    投稿 May 24 2018

    ELISA abreview for Anti-TRP1 antibody [TA99]

     Inconclusive
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Human Serum (melanoma patient serum)
    Specification
    melanoma patient serum
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Type
    Direct
    Read More

    Frau. Mirjam Fässler

    Verified customer

    投稿 Sep 12 2017

    Immunohistochemistry (Frozen sections) abreview for Anti-TRP1 antibody [TA99]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    0.1% triton, 2.5% goat serum, 2% BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 2% · Temperature: 25°C
    Sample
    Human Tissue sections (Human Skin)
    Specification
    Human Skin
    Permeabilization
    Yes - 0.1% tritonx-100
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 May 09 2014

    Question

    Dear Madam, Sir,

    For in vivo use we need to provide information on possible presence of murine viruses in TA99 Ab. Could you let me know if a test on murine viruses is done and what the results are.

    Regards,

    Read More
    Answer

    Thank you for contacting us and your interest in our products.

    We have not used the anti-TRP1 antibody [TA99] ab3312 in in vivo assays and would therefore not be able to guarantee the results. I would like to point out that the antibody has 0.1% azide as a preservative which is likely to be detrimental due to its toxicity. Ab80781 is a BSA and azide free preparation of the clone TA99.

    We have not confirmed if the preparation of ab3312 or ab80781 does contain any viruses. We do not expect it would but have not confirmed this.

    I hope this information has been of help. If I can be of any further assistance, please do not hesitate to let me know.

    Read More

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TRP1 antibody [TA99]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (SK-MEL-28)
    Specification
    SK-MEL-28
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% Triton-X in PBS
    Read More

    Abcam user community

    Verified customer

    投稿 Oct 18 2012

    Question

    Thanks for your kindly reply, after I contacted with this customer, he indicated he would like to change a free of replacement with ab34165 (Anti-Melanoma Associated Antigen 100+ / 7 kDa antibody [NKI/beteb]), could you please help this customer to add this antibody in our next order list?

    Thanks for your kindly help

    Best regards

    Read More
    Answer

    Thank you for confirming these details.

    We have added a free of charge unit of ab34165 to your order xx. This free of charge unit has been provided to replace the ab3312.

    Please wish the customer all the best of success with his research.

    Thank you for your collaboration. Please do not hesitate to contact us again, should you or the customer have any question.

    Read More

    Question

    Dear technical support team:

    This customer has purchased ab3312 (Anti-TRP1 antibody [TA99]) and has conducted the wb several times with human sample. The results show wrong band size, therefore, he wants to ask for your help to modify his experiment step, could you please offer any suggestion to help him?

    I also attached his image in this letter and his experiment step as follow:

    1. Order details:
    Abcam product code: ab3312
    Antibody storage conditions (temperature/reconstitution etc): +4℃

    2. Please describe the problem (high background, wrong band size, more bands, no band etc).
    Wrong band size

    3. On what material are you testing the antibody in WB?
    Species: human
    What's cell line or tissue: RPMI 7951 cell line
    Cell extract or Nuclear extract: Cell extract
    Purified protein or Recombinant protein: total protein

    3. The lysate
    How much protein was loaded: 25ug
    What lysis buffer was used:Tris 50mM,NaCl 150mM,EDTA 10mM,PMSF 1mM,SDS 0.1%,NP40 1%,triton 0.3% in Q-water
    What protease inhibitors were used: NO
    What loading buffer was used: 5X samplebuffer: 4ml 1.5M Tris-Cl pH6.8,10ml glycerol, 5ml beta-mercaptoethanol, 2g SDS, 1ml 1% bromophenol blue
    Phosphatase inhibitors :NO
    Did you heat the samples: temperature and time:95℃,10min

    4. Electrophoresis/Gel conditions/ Transfer conditions
    Reducing or non reducing gel: non reducing gel
    Reducing agent: NO
    Gel percentage : 10%
    Transfer conditions: (Type of membrane, Protein transfer verified): PVDF,

    5. Blocking conditions
    Buffer: TBST
    Blocking agent: milk, BSA, serum, what percentage: milk 5%
    Incubation time:1 h
    Incubation temperature: room temperature

    6. Primary Antibody
    Species: mouse
    Reacts against:
    At what dilution(s) have you tested this antibody:1:1000,1:2000
    What dilution buffer was used: TBST
    Incubation time: over night
    Incubation temperature: 4℃
    What washing steps were done: 5min X3

    7. Secondary Antibody
    Species: goat
    Reacts against:
    At what dilution(s) have you tested this antibody: 1:1000,1:3000
    Incubation time: 1 H
    Wash steps: 5min X3
    Fluorochrome or enzyme conjugate: HRP
    Do you know whether the problems you are experiencing come from the secondary?

    8. Detection method
    ECl, ECl+, other detection method: ECL

    9. Did you apply positive and negative controls along with the samples? Please specify.
    Negative control: H1299, JEG, C9 (human ovarian teratoma transformed epidermal carcinoma cell line)
    C9 reference: http://www.ncbi.nlm.nih.gov/pubmed/11087069Lu MH, et al. Proc Natl Sci Counc Repub China B. 2000:169-77

    10. Optimization attempts
    How many times have you tried the Western? 3 times
    Have you eliminated the possibility that any background bands could be due to the secondary antibody? (Run a “No primary” control): background clear
    Do you obtain the same results every time e.g. are background bands always in the same place? YES
    What steps have you altered? Alter Primary Antibody and Secondary Antibody dilution

    Could you please help this customer to solve the problem?

    Thanks for your kindly help

    Best regards

    Read More
    Answer

    Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

    The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

    Having reviewed the protocol details, I believe this product should have given satisfactory results. It appears that you may have received a faulty vial.

    The only recommandation I can make for a future test, are to use fresh samples with proteinase inhibitors (to exclude all possibility of protein degradation).

    I apologize for the inconvenience and am pleased to offer you a free of charge replacement, or credit note in compensation.

    Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

    Read More

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TRP1 antibody [TA99]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (B16 mouse melanoma cell line)
    Specification
    B16 mouse melanoma cell line
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton X-100
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. James Harris

    Verified customer

    投稿 Sep 27 2011

    1-10 of 12 Abreviews or Q&A

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