Key features and details
- Mouse monoclonal [N64A/36] to TRP 7
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
製品名Anti-TRP 7 antibody [N64A/36]
製品の詳細Mouse monoclonal [N64A/36] to TRP 7
アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
種交差性交差種: Mouse, Rat, Human
- Rat brain lysate
The clone number has been updated from S64A-36 to N64A/36, both clone numbers name the same antibody clone.
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保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
バッファーPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
精製度Protein G purified
The Abpromise guarantee
ICC/IF: Use at a concentration of 1 - 10 µg/ml.
IHC-P: Use at a concentration of 0.1 - 1 µg/ml.
IHC-Fr: Use at a concentration of 0.1 - 1 µg/ml.
WB: Use at a concentration of 1 - 10 µg/ml. Predicted molecular weight: 100 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
機能Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) (By similarity). May also be activated by intracellular calcium store depletion.
配列類似性Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC7 sub-subfamily.
Contains 4 ANK repeats.
- Information by UniProt
- Capacitative calcium channel TRPC7 antibody
- cation channel, subfamily C, member 7 antibody
- hTRP7 antibody
Western blot detection of Trp7 in Rat brain membrane lysates using ab93618 at 1:1000 dilution.
Cold 100% methanol for 10 minutes at -20°C fixed HaCaT cells labeling TRP 7 (green) using ab93618 at 1/100 dilution for 1 hour at room temperature. FITC Goat anti-mouse (green) was used as the secondary antibody at 1/50 dilution for 1 hour at room temperature. Nuclear staining is observed.
Bouin's-fixed, paraffin-embedded mouse backskin tissue stained for TRP 7 (green) using ab93618 at 1/100 dilution in immunohistochemical analysis. Goat Anti-Mouse was used as the secodary antibody at 1/50 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab93618 は 1 報の論文で使用されています。
- Nakipova OV et al. Store-operated Ca2+ entry supports contractile function in hearts of hibernators. PLoS One 12:e0177469 (2017). PubMed: 28531217