Anti-Trophoblast specific protein alpha 抗体 (ab104401)
Key features and details
- Rabbit polyclonal to Trophoblast specific protein alpha
- Suitable for: ICC/IF, WB
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-Trophoblast specific protein alpha antibody -
製品の詳細
Rabbit polyclonal to Trophoblast specific protein alpha -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse -
免疫原
Synthetic peptide corresponding to Mouse Trophoblast specific protein alpha aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab117405) -
ポジティブ・コントロール
- This antibody gave a positive signal in Mouse trophoblast tissue lysate as well as the following whole cell lysates: MEF1; NIH3T3; STO.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab104401の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (1) |
Use a concentration of 1 µg/ml.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.
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特記事項 |
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ICC/IF
Use a concentration of 1 µg/ml. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 14 kDa. |
ターゲット情報
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関連性
Trophoblast specific protein alpha is a marker of spongiotrophoblasts. -
参照データベース
- Entrez Gene: 21984 Mouse
- SwissProt: P13438 Mouse
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別名
- Tb1 antibody
- Tpbp antibody
- Tpbpa antibody
see all
画像
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All lanes : Anti-Trophoblast specific protein alpha antibody (ab104401) at 1 µg/ml
Lane 1 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Trophoblast Stem Cells (Mouse) Whole Cell Lysate
Lane 4 : STO (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 14 kDa
Additional bands at: 150 kDa, 255 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 14 minutes -
ICC/IF image of ab104401 stained MEF1 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96899, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab104401, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) MEF1 cells at 1µg/ml.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (25)
ab104401 は 25 報の論文で使用されています。
- Hufnagel A et al. Maternal but not fetoplacental health can be improved by metformin in a murine diet-induced model of maternal obesity and glucose intolerance. J Physiol 600:903-919 (2022). PubMed: 34505282
- Chen Z et al. Maternal exposure to CeO2NPs derails placental development through trophoblast dysfunction mediated by excessive autophagy activation. J Nanobiotechnology 20:131 (2022). PubMed: 35292031
- Zhang W et al. Rif1 and Hmgn3 regulate the conversion of murine trophoblast stem cells. Cell Rep 38:110570 (2022). PubMed: 35354046
- Rusidzé M et al. Loss of function of the maternal membrane oestrogen receptor ERα alters expansion of trophoblast cells and impacts mouse fertility. Development 149:N/A (2022). PubMed: 36239412
- Kinisu M et al. Klf5 establishes bi-potential cell fate by dual regulation of ICM and TE specification genes. Cell Rep 37:109982 (2021). PubMed: 34758315