Anti-TROP2 抗体 [EPR20043] (ab214488)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20043] to TROP2
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-TROP2 antibody [EPR20043]
TROP2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR20043] to TROP2 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HCT 116, PC-3 and MCF7 whole cell lysates; human breast cancer, skin, placenta and prostate cancer lysates, Mouse and rat skin and lung lysate, mouse kidney lysate. IHC-P: Human skin, breast and cervix cancer tissues; mouse and rat skin tissues. ICC/IF: MCF7 and HCT 116 cells. Flow Cyt (intra): MCF7 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR20043 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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KO cell lines
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab214488の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/60.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB | (1) |
1/2000. Detects a band of approximately 37-50 kDa (predicted molecular weight: 36 kDa).
|
ICC/IF | (1) |
1/100.
|
IHC-P | (4) |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ab214488 is recommended for mouse and rat in IHC |
特記事項 |
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Flow Cyt (Intra)
1/60. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/2000. Detects a band of approximately 37-50 kDa (predicted molecular weight: 36 kDa). |
ICC/IF
1/100. |
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. ab214488 is recommended for mouse and rat in IHC |
ターゲット情報
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機能
May function as a growth factor receptor. -
組織特異性
Placenta, pancreatic carcinoma cell lines. -
関連疾患
Defects in TACSTD2 are the cause of gelatinous drop-like corneal dystrophy (GDLD) [MIM:204870]; also known as lattice corneal dystrophy type III. GDLD is an autosomal recessive disorder characterized by grayish corneal amyloid deposits that cause severe visual impairment. -
配列類似性
Belongs to the EPCAM family.
Contains 1 thyroglobulin type-1 domain. -
翻訳後修飾
The N-terminus is blocked. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 4070 Human
- Entrez Gene: 56753 Mouse
- Entrez Gene: 494343 Rat
- Omim: 137290 Human
- SwissProt: P09758 Human
- SwissProt: Q8BGV3 Mouse
- SwissProt: Q6P9Z6 Rat
- Unigene: 23582 Human
see all -
別名
- Cell surface glycoprotein Trop 2 antibody
- Cell surface glycoprotein Trop-2 antibody
- Cell surface glycoprotein Trop2 antibody
see all
画像
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All lanes : Anti-TROP2 antibody [EPR20043] (ab214488) at 1/2000 dilution
Lane 1 : Wild-type MCF7 cell lysate
Lane 2 : TACSTD2 knockout MCF7 cell lysate
Lane 3 : HCT 116 cell lysate
Lane 4 : SK-BR-3 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 36-70 kDa why is the actual band size different from the predicted?Western blot: Anti-TACSTD2 antibody [EPR20043] (ab214488) staining at 1/2000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab214488 was shown to bind specifically to TACSTD2. A band was observed at 36-70 kDa in wild-type MCF7 cell lysates with no signal observed at this size in TACSTD2 knockout cell line. To generate this image, wild-type and TACSTD2 knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.|
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All lanes : Anti-TROP2 antibody [EPR20043] (ab214488) at 1/2000 dilution
Lane 1 : Mouse skin lysate at 20 µg
Lane 2 : Mouse kidney lysate at 20 µg
Lane 3 : Mouse lung lysate at 20 µg
Lane 4 : Rat skin lysate at 20 µg
Lane 5 : Rat lung lysate at 20 µg
Lane 6 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 36 kDa
Exposure time: 60 secondsBlocking and diluting buffer and concentration: 5% NFDM /TBST.
ab181602 was used as a GAPDH loading control.
Negative sample: A549 (PMID: 22419550).
TROP2 is highly glycosylated and appears as band around 37-50kDa.The molecular weight observed is consistent with what has been described in the literature (PMID: 23070813). -
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling TROP2 with ab214488 at 1/60 dilution (red) compared with aRabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. The permeabilization step was done using 90% methanol for 30 mins at -20 degrees.
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Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue labeling TROP2 with ab214488 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
Membrane staining on the tumor cells of human cervix cancer is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling TROP2 with ab214488 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing membrane and weakly cytoplasmic staining on MCF7 cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-TROP2 antibody [EPR20043] (ab214488) at 1/2000 dilution
Lane 1 : HCT 116 (Human colorectal carcinoma cell line) whole cell lysate
Lane 2 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 36 kDa
Observed band size: 37-50 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1/3: 2 seconds; Lane 2: 1 minute.
TROP2 is highly glycosylated and appears as band around 37-50kDa.The molecular weight observed is consistent with what has been described in the literature (PMID: 23070813)
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Immunohistochemical analysis of paraffin-embedded human breast tissue labeling TROP2 with ab214488 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
Membrane staining on the duct epithelium of human breast is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Lanes 1-3 : Anti-TROP2 antibody [EPR20043] (ab214488) at 1/2000 dilution
Lane 4 : Anti-TROP2 antibody [EPR20043] (ab214488) at 1/5000 dilution
Lane 1 : Human breast cancer lysate
Lane 2 : Human skin lysate
Lane 3 : Human placenta lysate
Lane 4 : Human prostate cancer lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 37-50 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1/3: 3 seconds; Lane 2: 5 seconds; Lane 4: 15 seconds.
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Immunohistochemical analysis of paraffin-embedded human skin tissue labeling TROP2 with ab214488 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
Membrane staining on the squamous epithelium of human skin is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded rat skin tissue labeling TACD2 with ab214488 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
Membrane staining on the squamous epithelium of rat skin is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
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Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling TROP2 with ab214488 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
Membrane staining on the squamous epithelium of mouse skin is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HCT 116 (Human colorectal carcinoma cell line) cells labeling TROP2 with ab214488 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing membrane staining on HCT 116 cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (14)
ab214488 は 14 報の論文で使用されています。
- Ito T et al. TROP2 Expression in Sebaceous and Sweat Gland Carcinoma. J Clin Med 11:N/A (2022). PubMed: 35160059
- Mikuteit M et al. Trophoblast Cell Surface Antigen 2 (Trop2) in Chromophobe Renal Cell Carcinoma. Oncology 100:331-336 (2022). PubMed: 35168242
- Fan Y et al. Head-to-Head Comparison of the Expression Differences of NECTIN-4, TROP-2, and HER2 in Urothelial Carcinoma and Its Histologic Variants. Front Oncol 12:858865 (2022). PubMed: 35515131
- Abu El Maaty MA et al. Hypoxia-mediated stabilization of HIF1A in prostatic intraepithelial neoplasia promotes cell plasticity and malignant progression. Sci Adv 8:eabo2295 (2022). PubMed: 35867798
- Ito T et al. Trop2 Expression in Extramammary Paget's Disease and Normal Skin. Int J Mol Sci 22:N/A (2021). PubMed: 34299325