Anti-TrkB 抗体 (ab18987)
Key features and details
- Rabbit polyclonal to TrkB
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-TrkB antibody
TrkB 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TrkB -
由来種
Rabbit -
特異性
The immunogen has 75% cross reactivity with TrKA and 68% cross reactivity with TrKC.
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アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Chicken
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免疫原
Synthetic peptide corresponding to Human TrkB. Surrounding amino acid 810 of human TrkB.
Database link: Q16620-1 -
ポジティブ･コントロール
- WB: HL-60 cell lysate.
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called 3593 TrkB Antibody. 3593-100 is the same size as the 100 µg size of ab18987.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.03% Proclin 300
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA, 0.19% EDTA disodium salt -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab18987の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (3) |
Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).
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| 特記事項 |
|---|
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WB
Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa). |
ターゲット情報
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機能
Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1. -
組織特異性
Isoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Contains 1 protein kinase domain. -
翻訳後修飾
Ligand-mediated auto-phosphorylation. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 4915 Human
- Entrez Gene: 18212 Mouse
- Entrez Gene: 25054 Rat
- Omim: 600456 Human
- SwissProt: Q16620 Human
- SwissProt: P15209 Mouse
- SwissProt: Q63604 Rat
- Unigene: 494312 Human
see all -
別名
- AI848316 antibody
- BDNF tropomyosine receptor kinase B antibody
- BDNF/NT 3 growth factors receptor antibody
see all
画像
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Western blot - Anti-TrkB antibody (ab18987)Anti-TrkB antibody (ab18987) + HL-60 cell lysate
Predicted band size: 92 kDa
Observed band size: >90 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (58)
ab18987 は 58 報の論文で使用されています。
- Tao J et al. Sfrp1 protects against acute myocardial ischemia (AMI) injury in aged mice by inhibiting the Wnt/ß-catenin signaling pathway. J Cardiothorac Surg 16:12 (2021). PubMed: 33468190
- Wei X et al. Inhibiting BDNF/TrkB.T1 receptor improves resiniferatoxin-induced postherpetic neuralgia through decreasing ASIC3 signaling in dorsal root ganglia. J Neuroinflammation 18:96 (2021). PubMed: 33874962
- Seo SY et al. Acupuncture Alleviates Anxiety and 22-kHz Ultrasonic Vocalizations in Rats Subjected to Repeated Alcohol Administration by Modulating the Brain-Derived Neurotrophic Factor/Corticotropin-Releasing Hormone Signaling Pathway. Int J Mol Sci 22:N/A (2021). PubMed: 33919862
- Marchese MJ et al. Perfluoroalkyl Substance Exposure and the BDNF Pathway in the Placental Trophoblast. Front Endocrinol (Lausanne) 12:694885 (2021). PubMed: 34394001
- Gowler PRW et al. Peripheral brain-derived neurotrophic factor contributes to chronic osteoarthritis joint pain. Pain 161:61-73 (2020). PubMed: 31479064