Tris Buffered Saline (TBS) Casein ELISA Reagent (ab171533)
製品の概要
-
製品名
Tris Buffered Saline (TBS) Casein ELISA Reagent
ELISA buffer ihc 試薬 製品一覧 -
アプリケーション
適用あり: ELISAmore details
適用なし: IHC-Fr or IHC-P -
特記事項
Abcam’s Tris Buffered Saline (TBS) Casein ELISA Reagent consists of many phosphoserine groups that function to bind Ca2+. The 10X solution is ready for dilution to specific application requirements. ab171533 contributes to the minimization of non-specific interactions of the secondary reagents with each other and with the primary solid phase binding sites. The high blocking efficiency of casein is due to its content of small molecular weight proteins. Tris Buffered Saline (TBS), pH 7.6, is a poor buffer below pH 7.5. Tris has the disadvantages of its reactivity as a primary amine and its appreciable solubility in organic solvents, which can lead to an accumulation in the biological phase of a reaction system. To avoid complex formation with ionic species such as calcium and magnesium in blood, tris buffers are preferable over phosphate buffers.
Storage Instructions:
Store at room temperature. This product should be clear and colorless in appearance. To minimize the risk of contamination, a fresh working solution should be prepared each day or store unused working solutions at the recommended storage temperature. If proper technique is not performed the possibility of introducing contaminants is always possible.Product Use:
ab171533 should be diluted 10X with reagent quality water. To produce a 1X working solution, dilute 1 mL of Tris Buffered Saline (TBS) Casein ELISA Reagent in 9 mL reagent quality water. Allow working solution to equilibrate to room temperature (25°C) prior to use. To use as a blocking reagent, treat target surface by covering completely with solution. To use working solution as a diluent, dilute antigen/antibody to desired concentration and treat surface by covering completely with solution. When solution is used for coating plates are obtained at +4°C.
製品の特性
-
製品の状態
Liquid -
保存方法
Please see notes section. -
バッファー
pH: 7
Constituent: Casein -
Concentration information loading...
関連製品
-
Related Products
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Phosphate Buffered Saline (PBS) Casein ELISA Reagent (ab171532)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
- AP Stability Conjugate Reagent (ab171536)
- HRP Stability Conjugate Reagent (ab171537)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab171533の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ELISA |
Use at an assay dependent concentration.
|
特記事項 |
---|
ELISA
Use at an assay dependent concentration. |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab171533 は論文での使用が確認できていません。