Triptolide from Tripterygium wilfordii, Anti-inflammatory agent (ab120720)
Key features and details
- Anti-inflammatory agent with multiple biological activities
- CAS Number: 38748-32-2
- Purity: > 99%
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Tripterygium wilfordii
製品の概要
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製品名
Triptolide from Tripterygium wilfordii, Anti-inflammatory agent -
製品の詳細
Anti-inflammatory agent with multiple biological activities -
別名
- PG490
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生理活性の詳細
Active ingredient of the vineTripterygium wilfodii Hook. f. Anti-inflammatory and immunosuppressive agent. Inhibits NF-κB transcriptional activity. Additionally, shows antiproliferative and pro-apoptotic activites. Downregulates RAC1, JAK/STAT 3 pathway and has anticancer properties.
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精製度
> 99% -
CAS 番号
38748-32-2 -
構造式
製品の特性
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体系名
(3bS,4aS,5aS,6R,6aR,7aS,7bS,8aS,8bS)-3b,4,4a,6,6a,7a,7b,8b,9,10-Decahydro-6-hydroxy-8b-methyl-6a-(1-methylethyl)trisoxireno[4b,5:6,7:8a,9]phenanthro[1,2-c]furan-1(3H)-one -
分子量
360.41 -
分子式
C20H24O6 -
保存方法
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解性
Soluble in DMSO to 100 mM -
使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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由来
Tripterygium wilfordii
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab120720の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Functional Studies |
Use at an assay dependent concentration.
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特記事項 |
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Functional Studies
Use at an assay dependent concentration. |
画像
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2D chemical structure image of ab120720, Triptolide from Tripterygium wilfordii, Anti-inflammatory agent
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ab22758 staining nucleolin in HeLa cells treated with Triptolide from Tripterygium wilfordii (ab120720), by ICC/IF. Changes in nuclear localization of nucleolin correlates with increased concentration of Triptolide from Tripterygium wilfordii, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120720 (Triptolide from Tripterygium wilfordii ) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab22758 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab120720 は 2 報の論文で使用されています。
- Gillen SL et al. Differential regulation of mRNA fate by the human Ccr4-Not complex is driven by coding sequence composition and mRNA localization. Genome Biol 22:284 (2021). PubMed: 34615539
- Zhang H et al. DEAD-Box Helicase 18 Counteracts PRC2 to Safeguard Ribosomal DNA in Pluripotency Regulation. Cell Rep 30:81-97.e7 (2020). PubMed: 31914400