Anti-TRIM5 alpha 抗体 (ab4389)
Key features and details
- Goat polyclonal to TRIM5 alpha
- Suitable for: WB, IHC-P
- Reacts with: Cat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-TRIM5 alpha antibody
TRIM5 alpha 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to TRIM5 alpha -
由来種
Goat -
特異性
This antibody is expected to recognise all three human isoforms. -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Cat, Human -
免疫原
Synthetic peptide:
ASGILVNVKEEVTC
, corresponding to N terminal amino acids 2-15 of Human RNF88. -
特記事項
Official Gene Symbol - TRIM5. GenBank Accession Number – NP_149023; NP_149083; NP_149084.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab4389の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 2 - 6 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
A 1 hour primary incubation is recommended for this product. |
| IHC-P |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 2 - 6 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa). A 1 hour primary incubation is recommended for this product. |
|
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Isoform Alpha is a retrovirus restriction factor, which mediates species-specific, early block to retrovirus infection. Targets retroviral capsid soon after entry into the cell, and prevents reverse transcription of the virus RNA genome. Isoform Alpha trimers may make multiple contacts with the hexameric lattice of CA proteins which constitute the surface of retrovirion core, and somehow inactivate the virus. Restricts efficiently infection by N-MLV, but not HIV-1. May have E3 ubiquitin-protein ligase activity. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger. -
ドメイン
The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme. -
翻訳後修飾
Ubiquitinates itself in a RING finger- and UBE2D2-dependent manner (in vitro). -
細胞内局在
Cytoplasm > P-body. Cytoplasmic bodies. - Information by UniProt
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参照データベース
- Entrez Gene: 85363 Human
- Omim: 608487 Human
- SwissProt: Q9C035 Human
- Unigene: 370515 Human
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別名
- RING finger protein 88 antibody
- RNF88 antibody
- TRIM5 antibody
see all
画像
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Western blot - Anti-TRIM5 alpha antibody (ab4389)Anti-TRIM5 alpha antibody (ab4389) at 2 µg/ml + Human placenta lysate in RIPA buffer at 35 µg
Predicted band size: 38 kDa
Observed band size: 38 kDa
Primary incubation was 1 hour. Detected by chemiluminescence. -
Western blot - Anti-TRIM5 alpha antibody (ab4389)Anti-TRIM5 alpha antibody (ab4389) at 1 µg/ml + Human Brain lysate (RIPA buffer, 35µg total protein per lane).
Predicted band size: 38 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (7)
ab4389 は 7 報の論文で使用されています。
- Jimenez-Guardeño JM et al. Immunoproteasome activation enables human TRIM5a restriction of HIV-1. Nat Microbiol 4:933-940 (2019). PubMed: 30886358
- Singh R et al. TRIM5a and TRIM22 are differentially regulated according to HIV-1 infection phase and compartment. J Virol 88:4291-303 (2014). PubMed: 24478420
- Battivelli E et al. Modulation of TRIM5alpha activity in human cells by alternatively spliced TRIM5 isoforms. J Virol 85:7828-35 (2011). WB ; Human . PubMed: 21632761
- Neagu MR et al. Potent inhibition of HIV-1 by TRIM5-cyclophilin fusion proteins engineered from human components. J Clin Invest 119:3035-47 (2009). ICC/IF ; Cat . PubMed: 19741300
- Yap MW et al. Restriction of foamy viruses by primate Trim5alpha. J Virol 82:5429-39 (2008). WB ; Human . PubMed: 18367529
- Yap MW et al. The design of artificial retroviral restriction factors. Virology 365:302-14 (2007). PubMed: 17493656
- Ohkura S et al. All three variable regions of the TRIM5alpha B30.2 domain can contribute to the specificity of retrovirus restriction. J Virol 80:8554-65 (2006). WB ; Human . PubMed: 16912305
