Anti-TRIM5 alpha 抗体 (ab4389)
Key features and details
- Goat polyclonal to TRIM5 alpha
- Suitable for: WB, IHC-P
- Reacts with: Cat, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-TRIM5 alpha antibody
TRIM5 alpha 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to TRIM5 alpha -
由来種
Goat -
特異性
This antibody is expected to recognise all three human isoforms. -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Cat, Human -
免疫原
Synthetic peptide:
ASGILVNVKEEVTC
, corresponding to N terminal amino acids 2-15 of Human RNF88. -
特記事項
Official Gene Symbol - TRIM5. GenBank Accession Number – NP_149023; NP_149083; NP_149084.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab4389 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 2 - 6 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa). A 1 hour primary incubation is recommended for this product. |
|
| IHC-P | Use at an assay dependent concentration. |
ターゲット情報
-
機能
Isoform Alpha is a retrovirus restriction factor, which mediates species-specific, early block to retrovirus infection. Targets retroviral capsid soon after entry into the cell, and prevents reverse transcription of the virus RNA genome. Isoform Alpha trimers may make multiple contacts with the hexameric lattice of CA proteins which constitute the surface of retrovirion core, and somehow inactivate the virus. Restricts efficiently infection by N-MLV, but not HIV-1. May have E3 ubiquitin-protein ligase activity. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger. -
ドメイン
The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme. -
翻訳後修飾
Ubiquitinates itself in a RING finger- and UBE2D2-dependent manner (in vitro). -
細胞内局在
Cytoplasm > P-body. Cytoplasmic bodies. - Information by UniProt
-
参照データベース
- Entrez Gene: 85363 Human
- Omim: 608487 Human
- SwissProt: Q9C035 Human
- Unigene: 370515 Human
-
別名
- RING finger protein 88 antibody
- RNF88 antibody
- TRIM5 antibody
see all
画像
-
Western blot - Anti-TRIM5 alpha antibody (ab4389)Anti-TRIM5 alpha antibody (ab4389) at 2 µg/ml + Human placenta lysate in RIPA buffer at 35 µg
Predicted band size: 38 kDa
Observed band size: 38 kDa
Primary incubation was 1 hour. Detected by chemiluminescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM5 alpha antibody (ab4389)Ab4389 (1ug/ml) staining human RNF88 in human tonsil by Immunohistochemistry using paraffin embedded tissue. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
A) positive cells next to a secondary follicle
B) positive cells below tonsillar epithelium.
In paraffin embedded Human Tonsil, ab4389 stains a group of cells in the interfollicular area, below the epithelium of tonsillar criptae and weak in germinal centre.
-
Western blot - Anti-TRIM5 alpha antibody (ab4389)Anti-TRIM5 alpha antibody (ab4389) at 1 µg/ml + Human Brain lysate (RIPA buffer, 35µg total protein per lane).
Predicted band size: 38 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
プロトコール
データシートおよび資料
参考文献 (7)
ab4389 は 7 報の論文で使用されています。
- Jimenez-Guardeño JM et al. Immunoproteasome activation enables human TRIM5a restriction of HIV-1. Nat Microbiol 4:933-940 (2019). PubMed: 30886358
- Singh R et al. TRIM5a and TRIM22 are differentially regulated according to HIV-1 infection phase and compartment. J Virol 88:4291-303 (2014). PubMed: 24478420
- Battivelli E et al. Modulation of TRIM5alpha activity in human cells by alternatively spliced TRIM5 isoforms. J Virol 85:7828-35 (2011). WB ; Human . PubMed: 21632761
- Neagu MR et al. Potent inhibition of HIV-1 by TRIM5-cyclophilin fusion proteins engineered from human components. J Clin Invest 119:3035-47 (2009). ICC/IF ; Cat . PubMed: 19741300
- Yap MW et al. Restriction of foamy viruses by primate Trim5alpha. J Virol 82:5429-39 (2008). WB ; Human . PubMed: 18367529
- Yap MW et al. The design of artificial retroviral restriction factors. Virology 365:302-14 (2007). PubMed: 17493656
- Ohkura S et al. All three variable regions of the TRIM5alpha B30.2 domain can contribute to the specificity of retrovirus restriction. J Virol 80:8554-65 (2006). WB ; Human . PubMed: 16912305
