Anti-TRIM35 抗体 (ab272582)
Key features and details
- Rabbit polyclonal to TRIM35
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TRIM35 antibody -
製品の詳細
Rabbit polyclonal to TRIM35 -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P Human
-
免疫原
Recombinant fragment corresponding to Human TRIM35 aa 150-300.
Database link: Q9UPQ4 -
ポジティブ･コントロール
- WB: HEK-293T cells. Human tonsil tissue. IHC-P: Human appendix tissue. ICC/IF: U-251 MG cells.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272582 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
|
| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
May play a role as a tumor suppressor. Implicated in the cell death mechanism. -
配列類似性
Belongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger. -
ドメイン
The RING finger domain and the coiled-coil region are required for the apoptosis-inducing activity. -
細胞内局在
Cytoplasm. Nucleus. Found predominantly in cytoplasm with a granular distribution. Found in punctuate nuclear bodies. - Information by UniProt
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参照データベース
- Entrez Gene: 23087 Human
- SwissProt: Q9UPQ4 Human
- Unigene: 104223 Human
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別名
- 0710005M05Rik antibody
- A430106H13Rik antibody
- AW046487 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM35 antibody (ab272582)
Paraffin-embedded formalin-fixed Human appendix tissue stained for TRIM35 using ab272582 at 1/500 in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-TRIM35 antibody (ab272582)PFA fixed, Triton X-100 permeablized human cell line U-251 MG labeling TRIM35 (green) using ab272582 at 2 ug/ml in ICC/IF analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab272582 は論文での使用が確認できていません。
