Triiodothyronine (T3) ELISA Kit (ab108685)
Key features and details
- Sensitivity: 0.05 ng/ml
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
製品の概要
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製品名
Triiodothyronine (T3) ELISA Kit
Triiodothyronine (T3) キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 40 < 10.7% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 3 < 9.1% -
サンプルの種類
Serum, Hep Plasma, Cit plasma -
アッセイタイプ
Competitive -
検出感度
0.05 ng/ml -
添加回収試験
97.5 %
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ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Triiodothyronine (T3) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of total Triiodothyronine in serum and plasma.
A 96-well plate has been precoated with anti-T3. Samples and the T3-HRP conjugate are added to the wells, where Triiodothyronine in the sample competes with the addedT3-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Triiodothyronine in the sample and the intensity is measured at 450 nm.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 11X T3-HRP Conjugate 1 x 1.4ml 50X Wash Solution 1 x 20ml Anti-T3 IgG Coated Microplate (12 x 8 wells) 1 unit Conjugate Buffer 1 x 12.5ml Cover foils 1 unit Stop Solution 1 x 15ml Strip holder 1 unit T3 Standard 0 - 0.0 ng/mL 1 x 1ml T3 Standard 1 - 0.5 ng/mL 1 x 1ml T3 Standard 2 - 1.0 ng/mL 1 x 1ml T3 Standard 3 - 2.5 ng/mL 1 x 1ml T3 Standard 4 - 5.0 ng/mL 1 x 1ml T3 Standard 5 - 7.5 ng/mL 1 x 1ml TMB Substrate Solution 1 x 15ml -
研究分野
画像
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Typical Standard Curve
Representative Standard Curve using ab108685
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab108685 は 2 報の論文で使用されています。
- Tani N et al. Thyroid-related hormones as potential markers of hypoxia/ischemia. Hum Cell 33:545-558 (2020). PubMed: 32146707
- Gil-Cayuela C et al. Myocardium of patients with dilated cardiomyopathy presents altered expression of genes involved in thyroid hormone biosynthesis. PLoS One 13:e0190987 (2018). PubMed: 29320567