Anti-TRIF 抗体 (ab13810)
Key features and details
- Rabbit polyclonal to TRIF
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-TRIF antibody
TRIF 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TRIF -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat -
免疫原
Synthetic peptide within Mouse TRIF aa 100-200. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q80UF7 -
ポジティブ・コントロール
- WB: Mouse spleen, intestine and RAW 264.7 cell lysates. IHC-P: Mouse spleen tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab13810の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (3) |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 79.23 kDa.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
IHC-P |
1/200.
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特記事項 |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 79.23 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
IHC-P
1/200. |
ターゲット情報
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機能
Involved in innate immunity against invading pathogens. Adapter used by TLR3 and TLR4 (through TICAM2) to mediate NF-kappa-B and interferon-regulatory factor (IRF) activation, and to induce apoptosis. Ligand binding to these receptors results in TRIF recruitment through its TIR domain. Distinct protein-interaction motifs allow recruitment of the effector proteins TBK1, TRAF6 and RIPK1, which in turn, lead to the activation of transcription factors IRF3 and IRF7, NF-kappa-B and FADD respectively. -
組織特異性
Ubiquitously expressed but with higher levels in liver. -
配列類似性
Contains 1 TIR domain. -
ドメイン
The N-terminal region is essential for activation of the IFNB promoter activity. -
翻訳後修飾
Phosphorylated by TBK1. - Information by UniProt
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参照データベース
- Entrez Gene: 106759 Mouse
- Entrez Gene: 363328 Rat
- SwissProt: Q80UF7 Mouse
- Unigene: 203952 Mouse
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別名
- IIAE6 antibody
- MGC35334 antibody
- MyD88 3 antibody
see all
画像
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All lanes : Anti-TRIF antibody (ab13810) at 1/1000 dilution
Lane 1 : Mouse spleen lysate with immunizing peptide
Lane 2 : Mouse spleen lysate
Predicted band size: 79.23 kDa -
Immunohistochemistrical analysis of a FFPE tissue section of mouse spleen using 1/200 dilution of ab13810. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.
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All lanes : Anti-TRIF antibody (ab13810) at 1/1000 dilution
Lane 1 : Mouse intestine lysate
Lane 2 : Mouse spleen lysate
Lane 3 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate
Predicted band size: 79.23 kDa
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (46)
ab13810 は 46 報の論文で使用されています。
- Kim YK et al. Differential susceptibility to lipopolysaccharide affects the activation of toll-like-receptor 4 signaling in THP-1 cells and PMA-differentiated THP-1 cells. Innate Immun 28:122-129 (2022). PubMed: 35612375
- Muendlein HI et al. ZBP1 promotes inflammatory responses downstream of TLR3/TLR4 via timely delivery of RIPK1 to TRIF. Proc Natl Acad Sci U S A 119:e2113872119 (2022). PubMed: 35666872
- Hornigold K et al. Age-related decline in the resistance of mice to bacterial infection and in LPS/TLR4 pathway-dependent neutrophil responses. Front Immunol 13:888415 (2022). PubMed: 36090969
- Liu Y et al. Antiviral activity of portulaca oleracea L. extracts against porcine epidemic diarrhea virus by partial suppression on myd88/NF-?b activation in vitro. Microb Pathog 154:104832 (2021). PubMed: 33781871
- Nihon-Yanagi Y et al. Immunohistochemical Analysis of Toll-Like Receptors, MyD88, and TRIF in Human Papillary Thyroid Carcinoma and Anaplastic Thyroid Carcinoma. J Thyroid Res 2021:4226491 (2021). PubMed: 34306609