製品の概要

  • 製品名
    Trichrome Stain Kit (Connective Tissue Stain)
  • 製品の概要

    Trichrome Stain Kit (Connective Tissue Stain) ab150686 is intended for use in the histological visualization of collagenous connective tissue fibers in tissue sections.


    The trichrome stain kit may be used on formalin-fixed, paraffin-embedded. For frozen sections, it will require optimization by the user.


    Trichrome stain protocol summary:
    - deparaffinize sections if necessary
    - preheat Bouin's Fluid to 54-64ºC
    - incubate slide in preheated Bouin's Fluid for 60 min and cool for 10 min
    - rinse in water
    - incubate slide in Weigert's Iron Hematoxylin for 5 min
    - rinse in water
    - incubate slide in Biebrich Scarlet / Acid Fuchsin solution for 15 min
    - rinse in water
    - differentiate in phosphomolybdic / phosphotungstic acid solution for 10-15 min
    - incubate slide in Aniline Blue solution for 5-10 min
    - rinse in water
    - incubate slide in acetic acid solution for 3-5 min
    - dehydrate, clear and mount


    Other products for staining tissue sections


    Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

  • 特記事項

    Staining Interpretation

    Collagen Blue
    Muscle Fibers    Red
    Nuclei Dark Red to Black/Blue

    Control Tissue: Lung, Uterus, Small Intestine, Stomach.

製品の特性

  • 保存方法
    Store at room temperature. Please refer to protocols.
  • 内容 100 tests
    Acetic Acid Solution 1 x 125ml
    Aniline Blue Solution 1 x 125ml
    Biebrich Scarlet/Acid Fuchsin Solution 1 x 125ml
    Bouin's Fluid 1 x 125ml
    Phosphomolybdic/Phosphotungstic acid solution 1 x 125ml
    Weigert's Iron, Hematoxylin (A) 1 x 125ml
    Weigert's Iron, Hematoxylin (B) 1 x 125ml
  • 研究分野

画像

  • ab150686 Trichrome Stain Kit (Connective Tissue Stain) staining formalin-fixed-paraffin embedded human muscle.

  • ab150686 (Trichrome Stain) staining collagen (blue) and muscle fibre (red) in formalin fixed paraffin embedded normal human placenta. Nuclei are counterstained with Weigert’s hematoxylin (blue)

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • A paraffin-embedded section of  healthy mouse spleen stained with ab150686 - Trichrome Stain Kit (Connective Tissue Stain). The sample was deparaffinized by heating to 60°C for 1 hour and then deparaffinization following a standard protocol. Sections were then fixed with Bouin's solution (included in the kit) for 1 hour at 60°C in humid conditions.

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参考文献

This product has been referenced in:
  • Yen JH  et al. Activation of dynamin-related protein 1 - dependent mitochondria fragmentation and suppression of osteosarcoma by cryptotanshinone. J Exp Clin Cancer Res 38:42 (2019). Read more (PubMed: 30691497) »
  • Ponticorvo A  et al. Evaluating clinical observation versus Spatial Frequency Domain Imaging (SFDI), Laser Speckle Imaging (LSI) and thermal imaging for the assessment of burn depth. Burns 45:450-460 (2019). Read more (PubMed: 30327232) »
See all 23 Publications for this product

レビューと Q&A

1-4 of 4 Abreviews or Q&A

GOOD KIT, it works well and everything is provided

Excellent Excellent 5/5 (Ease of Use)
Abreviews
GOOD KIT, it works well and everything is provided. The kit has all the reagents for the procedure. You only need to have Xylene and Ethanol and the basic for miscopy (slides etc.).

Dr. Francesco Elia Marino

Verified customer

投稿 Jan 31 2018

Answer

The product ab150686 can be used to stain connective tissues irrespective of species. It will be fine for human samples.

Read More

Mouse spleen stain

Excellent Excellent 5/5 (Ease of Use)
Abreviews
The sample is a section of parafin mouse spleen in a healthy state. The sample have been deparafined by heating it up to 60ºC during 1 hour and then following a standard deparafination protocol until H2O, then fixed with Bouin's solution (included in the kit) 1 hour at 60ºC in humid conditions.

Mr. Javier Rodriguez

Verified customer

投稿 Feb 28 2014

Human xenograft Ewin Sarcoma

Good Good 4/5 (Ease of Use)
Abreviews
I have used this kit to stain samples of a Xenograft of Ewin sarcoma cells within a swiss nude mice in order to detect the collagen I fibers present in the tumour bulk.
The kit is ready to use and easily handling.

Mr. Javier Rodriguez

Verified customer

投稿 Jan 29 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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