Key features and details
- Rabbit polyclonal to TRF2
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
TRF2 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to TRF2
特異性This antibody detects bands of 65 and 69 kDa which correspond to TRF2.
アプリケーション適用あり: ICC/IF, WBmore details
- HeLa nuclear extract; HeLa whole cell lysate; HeLa nuclear lysate; HeLa whole cell lysate;
Human telomeres are composed of long arrays of TTAGGG repeats that form a nucleoprotein complex required for the protection and replication of chromosome ends. TRF2 is ubiquitously expressed, bound specifically to duplex TTAGGG repeats in vitro, and localized to all human telomeres in metaphase chromosomes. It is expressed as 2 proteins of 65 and 69 kD. Affinity-purified antibodies specific for anti-TRF2 labeled the telomeres of intact human chromosomes, strengthening the correlation between occurrence of telobox and telomere-repeat recognition in vivo. TRF2 is likely to bind to double-stranded telomeric DNA as a homodimer.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
精製度Immunogen affinity purified
一次抗体 備考Human telomeres are composed of long arrays of TTAGGG repeats that form a nucleoprotein complex required for the protection and replication of chromosome ends. TRF2 is ubiquitously expressed, bound specifically to duplex TTAGGG repeats in vitro, and localized to all human telomeres in metaphase chromosomes. It is expressed as 2 proteins of 65 and 69 kD. Affinity-purified antibodies specific for anti-TRF2 labeled the telomeres of intact human chromosomes, strengthening the correlation between occurrence of telobox and telomere-repeat recognition in vivo. TRF2 is likely to bind to double-stranded telomeric DNA as a homodimer.
Our Abpromise guarantee covers the use of ab4182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||1/1000. Detects a band of approximately 65, 69 kDa (predicted molecular weight: 55.5 kDa).|
機能Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.
組織特異性Ubiquitous. Highly expressed in spleen, thymus, prostate, uterus, testis, small intestine, colon and peripheral blood leukocytes.
配列類似性Contains 1 HTH myb-type DNA-binding domain.
ドメインThe TRFH dimerization region mediates the interaction with DCLRE1B/Apollo but not TINF2.
翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
細胞内局在Nucleus. Chromosome > telomere. Colocalizes with telomeric DNA in interphase cells and is located at chromosome ends during metaphase.
- Information by UniProt
- Telomeric DNA binding protein antibody
- Telomeric DNA-binding protein antibody
- Telomeric repeat binding factor 2 antibody
All lanes : Anti-TRF2 antibody (ab4182) at 1 µg/ml
Lane 1 : HeLa nuclear extract
Lane 2 : HeLa whole cell lysate
Lane 3 : HeLa nuclear lysate with Human TRF2 peptide (ab13765) at 1 µg/ml
Lane 4 : HeLa whole cell lysate with Human TRF2 peptide (ab13765) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
Predicted band size: 55.5 kDa
ICC/IF image of ab4182 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab4182, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
ab4182 staining TRF2 in human Breast carcinoma cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton-x100 and blocking with 2% serum was performed for 30 minutes at 370C. Samples were incubated with primary antibody at 1/100 dilution in PBS for 2 hours at 37°C. An Alexa Fluor® 594 conjugated goat monoclonal to rabbit IgG was used at 1/75 dilution as secondary antibody.
ab4182 は 5 報の論文で使用されています。
- Wang J et al. Bacterial quorum-sensing signal IQS induces host cell apoptosis by targeting POT1-p53 signalling pathway. Cell Microbiol 21:e13076 (2019). PubMed: 31254473
- Bilsland AE et al. A Novel Pyrazolopyrimidine Ligand of Human PGK1 and Stress Sensor DJ1 Modulates the Shelterin Complex and Telomere Length Regulation. Neoplasia 21:893-907 (2019). PubMed: 31401411
- Ilicheva N et al. The karyosphere capsule in Rana temporaria oocytes contains structural and DNA-binding proteins. Nucleus 9:516-529 (2018). PubMed: 30272509
- Lian S et al. PRL-3 promotes telomere deprotection and chromosomal instability. Nucleic Acids Res 45:6546-6571 (2017). PubMed: 28482095
- Mao Z et al. TRF2 is required for repair of nontelomeric DNA double-strand breaks by homologous recombination. Proc Natl Acad Sci U S A 104:13068-73 (2007). PubMed: 17670947