Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cell)
Loading amount
100 µg
Specification
HeLa cell
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%
Other product details
Dilution
1/100
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% SKIN MILK IN TBST
Secondary antibody
Name
Non-Abcam antibody was used: GOAT ANTI-RABBIT HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ecl+
Exposure
30 minute(s) and 0 second(s)
Bands
Specific: 0 kDa Non-specific: 55, 75, 80 kDa
Positive control
TRF1 siRNA Transfection sample
Additional data
Additional Notes
no specific band,
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Geun Hyoung Ha
Verified customer
投稿 May 27 2009