Application
Western blot
Sample
Human Cell lysate - whole cell (Hela CELLS)
Loading amount
50 µg
Specification
Hela CELLS
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Additional data
Abcam response
Thank you for your honest and valuable feedback. As this is a low-copy nuclear protein, it may be necessary to enrich the nuclear portion of the lysate using fractionating to obtain an optimal signal with this antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Geun-Hyoung Ha
Verified customer
投稿 Apr 30 2009