Rabbit monoclonal [EPR3973] to Trefoil Factor 3
Synthetic peptide within Human Trefoil Factor 3 aa 1-100. The exact sequence is proprietary.
Human uterus cancer and colon cancer lysates; Human colon tissue.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.01% Sodium azide Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 9 kDa.
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/50 - 1/100.
Use at an assay dependent concentration.
Is unsuitable for IP.
Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).
Expressed in goblet cells of the intestines and colon (at protein level). Expressed by goblet cells of small and large intestinal epithelia and also by the uterus.
Contains 1 P-type (trefoil) domain.
Secreted > extracellular space > extracellular matrix > apical lamina.
Information by UniProt
Intestinal trefoil factor antibody
Western blot - Anti-Trefoil Factor 3 antibody [EPR3973] (ab109104)
All lanes : Anti-Trefoil Factor 3 antibody [EPR3973] (ab109104) at 1/1000 dilution Lane 1 : Human uterus cancer lysate Lane 2 : Human colon cancer lysate Lysates/proteins at 10 µg per lane. Predicted band size: 9 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trefoil Factor 3 antibody [EPR3973] (ab109104)
Immunohistochemical analysis of Trefoil Factor 3 in paraffin-embedded Human colon tissue using ab109104 at 1/500 dilution.
Flow Cytometry - Anti-Trefoil Factor 3 antibody [EPR3973] (ab109104)
Flow Cytometry analysis of HT-29 (human colorectal adenocarcinoma) cells labeling Trefoil Factor 3 with unpurified ab109104 at 1/100 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor
® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"