Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (spleen)
Antigen retrieval step
None
Permeabilization
No
Specification
spleen
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Formaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% FBS/PBS
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Molecular Probes Goat anti-Rb-488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Tried this antibody on mouse and rat spleen tissue. Looks specific for the rat spleen. Appears to be on T-cells only not B-cells.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Nancy Nutile-McMenemy
Verified customer
投稿 Jul 06 2007