Anti-TRAP/CD40L 抗体 (ab2391)
Key features and details
- Rabbit polyclonal to TRAP/CD40L
- Suitable for: Flow Cyt, ICC/IF, ELISA, WB, IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-TRAP/CD40L antibody
TRAP/CD40L 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TRAP/CD40L -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt, ICC/IF, ELISA, WB, IHC-P, IHC-Frmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to TRAP/CD40L. surrounding amino acid 246 of human CD40 Ligand
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ポジティブ･コントロール
- Human muscle tissue sections
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.03% Proclin 300
Constituents: PBS, 30% Glycerol, 0.5% BSA, 0.015% EDTA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab2391 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt | 1/200. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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| ICC/IF | 1/200. See Abreview | |
| ELISA | Use a concentration of 0.5 - 1 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | 1/100. Fix with acetone -20°C for 10 min (provides a better signal than unfixed tissue; PFA 4% abolishes the signal). Incubate primary for 24h at 4°C. Use amplification (indirect IF). |
ターゲット情報
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機能
Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. -
組織特異性
Specifically expressed on activated CD4+ T-lymphocytes. -
関連疾患
Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. -
配列類似性
Belongs to the tumor necrosis factor family. -
翻訳後修飾
The soluble form derives from the membrane form by proteolytic processing.
N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
Not O-glycosylated. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 959 Human
- Entrez Gene: 21947 Mouse
- Entrez Gene: 84349 Rat
- Omim: 300386 Human
- SwissProt: P29965 Human
- SwissProt: P27548 Mouse
- SwissProt: Q9Z2V2 Rat
- Unigene: 592244 Human
see all -
別名
- CD 40L antibody
- CD154 antibody
- CD40 antigen ligand antibody
see all
画像
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Flow Cytometry - Anti-TRAP/CD40L antibody (ab2391)Image courtesy of an anonymous Abreview.ab2391 staining TRAP/CD40L in Human platelet cells by Flow cytometry.
Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/200 dilution and incubated for 16 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated chicken anti-rabbit IgG (H+L) at a 1/500 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-TRAP/CD40L antibody (ab2391)Image courtesy of an AbReview submitted by Dr. Mahesh ShivananjappaImmunofluorescent analysis of paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells TRAP/CD40L with ab2391 at 1/200 dilution, followed by Chicken Anti-Rabbit IgG H&L (Alexa Fluor® 488 secondary antibody at 1/750 dilution (green). WGA is detected with Alexa Fluor® 568 Wheat Germ Agglutinin (red). The nuclear counter stain is DAPI (blue).
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Western blot - Anti-TRAP/CD40L antibody (ab2391)All lanes : Anti-TRAP/CD40L antibody (ab2391)
Lane 1 : Jurkat cell lysates
Lane 2 : Mouse small intestine lysate
Lane 3 : Rat kidney Lysate -
Immunohistochemistry (Frozen sections) - Anti-TRAP/CD40L antibody (ab2391)This image is courtesy of an Abreview submitted by Jens Schmidtab2391 at a 1/100 dilution staining TRAP/CD40L from human inflamed muscle tissue sections by immunohistochemistry (frozen sections). The tissue was acetone fixed (this provides a better result than unfixed tissue) and then incubated with the antibody for 24 hours at 4 °C. Alexa-Fluor ® 594 was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP/CD40L antibody (ab2391)This image is courtesy of an Abreview submitted by Ms Nancy Nutile-McMenemyab2391 at 1/500 staining rat spleen tissue sections by IHC-P. The tissue was fomraldehyde fixed and blocked with serum prior to incubation with the antibody for 18 hours. An Alexa-Fluor® 488 conjugated goat anti-rabbit was used as the secondary.
プロトコール
参考文献
ab2391 は 6 報の論文で使用されています。
- Carriba P & Davies AM How CD40L reverse signaling regulates axon and dendrite growth. Cell Mol Life Sci N/A:N/A (2020). PubMed: 32506167
- Shen DS et al. Aspirin eugenol ester inhibits agonist-induced platelet aggregation in vitro by regulating PI3K/Akt, MAPK and Sirt 1/CD40L pathways. Eur J Pharmacol 852:1-13 (2019). PubMed: 30797789
- Howard L et al. CD40 forward signalling is a physiological regulator of early sensory axon growth. Development 146:N/A (2019). PubMed: 31488565
- Bagi Z et al. Extracellular vesicle integrins act as a nexus for platelet adhesion in cerebral microvessels. Sci Rep 9:15847 (2019). PubMed: 31676801
- Carriba P & Davies AM CD40 is a major regulator of dendrite growth from developing excitatory and inhibitory neurons. Elife 6:N/A (2017). PubMed: 29111976
- Ye QX et al. Indoleamine 2,3-dioxygenase and inducible nitric oxide synthase mediate immune tolerance induced by CTLA4Ig and anti-CD154 hematopoietic stem cell transplantation in a sensitized mouse model. Exp Ther Med 14:1884-1891 (2017). PubMed: 28962099
