Anti-Translin/TSN 抗体 (ab96155)
Key features and details
- Rabbit polyclonal to Translin/TSN
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Translin/TSN antibody
Translin/TSN 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Translin/TSN -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Chicken, Cow, Zebrafish, Orangutan -
免疫原
Synthetic peptide corresponding to Human Translin/TSN aa 150 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab107811) -
ポジティブ・コントロール
- This antibody gave a positive signal in the following human tissue lysates: Brain; Testis; Lymph Node as well as the following human whole cell lysates: Jurkat; HeLa; HepG2; MCF7.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96155の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 1 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
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特記事項 |
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ICC/IF
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). |
ターゲット情報
- Information by UniProt
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参照データベース
- Entrez Gene: 7247 Human
- Entrez Gene: 22099 Mouse
- Entrez Gene: 60381 Rat
- Omim: 600575 Human
- SwissProt: P79769 Chicken
- SwissProt: Q08DM8 Cow
- SwissProt: Q15631 Human
- SwissProt: Q62348 Mouse
see all -
製品の状態
Cellular Localisation: Cytoplasm. Nucleus -
別名
- BCLF 1 antibody
- BCLF1 antibody
- Component 3 of promoter of RISC antibody
see all
画像
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All lanes : Anti-Translin/TSN antibody (ab96155) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : TSN knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDaLanes 1-3: Merged signal (red and green). Green - ab96155 observed at 26 kDa. Red - loading control ab8245 observed at 36 kDa.
ab96155 Anti-Translin/TSN antibody was shown to specifically react with Translin/TSN in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264778 (knockout cell lysate ab258255) was used. Wild-type and Translin/TSN knockout samples were subjected to SDS-PAGE. ab96155 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-Translin/TSN antibody (ab96155) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 5 : Human brain tissue lysate - total protein (ab29466)
Lane 6 : Human testis tissue lysate - total protein (ab30257)
Lane 7 : Human lymph node tissue lysate - total protein (ab29871)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute -
ICC/IF image of ab96155 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab96155 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab96155 は論文での使用が確認できていません。