Anti-Transglutaminase 2 抗体 [EP2957] (ab109200)
![Western blot - Anti-Transglutaminase 2 antibody [EP2957] (ab109200)](https://www.abcam.co.jp/ps/products/109/ab109200/Images/ab109200-390845-anti-transglutaminase-2-antibody-ep2957-western-blot-wildtype-a549-lysates.jpg "Western blot - Anti-Transglutaminase 2 antibody [EP2957] (ab109200)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2957] to Transglutaminase 2
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Transglutaminase 2 antibody [EP2957]
Transglutaminase 2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP2957] to Transglutaminase 2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: U87-MG, A549, HeLa and HUVEC cell lysates. IHC-P: Human kidney tissue. IHC-Fr: Mouse kidney and heart tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP2957 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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KO cell pellets
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109200の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/10000 - 1/50000. Predicted molecular weight: 77 kDa.
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/10000 - 1/50000. Predicted molecular weight: 77 kDa. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. -
配列類似性
Belongs to the transglutaminase superfamily. Transglutaminase family. - Information by UniProt
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参照データベース
- Entrez Gene: 7052 Human
- Entrez Gene: 21817 Mouse
- Omim: 190196 Human
- SwissProt: P21980 Human
- SwissProt: P21981 Mouse
- Unigene: 517033 Human
- Unigene: 330731 Mouse
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別名
- ALPHA SUBUNIT antibody
- C polypeptide antibody
- EC 2.3.2.13 antibody
see all
画像
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Western blot - Anti-Transglutaminase 2 antibody [EP2957] (ab109200)All lanes : Anti-Transglutaminase 2 antibody [EP2957] (ab109200) at 1/10000 dilution
Lane 1 : Wild-type A549 cell lysate
Lane 2 : TGM2 Knockout A549 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 77 kDa
Observed band size: 77 kDaLanes 1-2: Merged signal (red and green). Green - ab109200 observed at 77 kDa. Red - loading control ab8245 observed at 37 kDa.
ab109200 Anti-Transglutaminase 2 antibody [EP2957] was shown to specifically react with Transglutaminase 2 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267110 (knockout cell lysate ab257087) was used. Wild-type and Transglutaminase 2 knockout samples were subjected to SDS-PAGE. ab109200 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Transglutaminase 2 antibody [EP2957] (ab109200)](https://www.abcam.co.jp/ps/products/109/ab109200/Images/ab109200-386636-anti-transglutaminase-2-antibody-ep2957-western-blot-human-lysate.jpg)
Western blot - Anti-Transglutaminase 2 antibody [EP2957] (ab109200)All lanes : Anti-Transglutaminase 2 antibody [EP2957] (ab109200) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : TGM2 knockout HeLa cell lysate
Lane 3 : Wild-type A549 cell lysate
Lane 4 : TGM2 knockout A549 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 77 kDa
Observed band size: 77 kDaLanes 1-4: Merged signal (red and green). Green - ab109200 observed at 77 kDa. Red - loading control ab8245 observed at 37 kDa.
ab109200 Anti-Transglutaminase 2 antibody [EP2957] was shown to specifically react with Transglutaminase 2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265245 (knockout cell lysate ab257085) was used. Wild-type and Transglutaminase 2 knockout samples were subjected to SDS-PAGE. ab109200 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Transglutaminase 2 antibody [EP2957] (ab109200)](https://www.abcam.co.jp/ps/products/109/ab109200/Images/ab109200-349465-anti-transglutaminase-2-antibody-ep2957-western-blot-knock-out-og-a549-tgm2-hela-huvec-humanlysates.jpg)
Western blot - Anti-Transglutaminase 2 antibody [EP2957] (ab109200)All lanes : Anti-Transglutaminase 2 antibody [EP2957] (ab109200) at 1/1000 dilution
Lane 1 : Wild-type A549 whole cell lysate
Lane 2 : TGM2 knockout A549 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HUVEC whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 77 kDaLanes 1 - 4: Merged signal (red and green). Green - ab109200 observed at 77 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109200 was shown to recognize TGM2 in wild-type A549 cells as signal was lost at the expected MW in TGM2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TGM2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab109200 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Transglutaminase 2 antibody [EP2957] (ab109200)](https://www.abcam.co.jp/ps/products/109/ab109200/Images/ab109200-121826-anti-transglutaminase-2-antibody-ep2957-western-blot.jpg)
Western blot - Anti-Transglutaminase 2 antibody [EP2957] (ab109200)All lanes : Anti-Transglutaminase 2 antibody [EP2957] (ab109200) at 1/10000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HUVEC cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 77 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [EP2957] (ab109200)](https://www.abcam.co.jp/ps/products/109/ab109200/Images/ab109200-121825-anti-transglutaminase-2-antibody-ep2957-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [EP2957] (ab109200)ab109200, at 1/100 dilution, staining Transglutaminase 2 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Transglutaminase 2 antibody [EP2957] (ab109200)](https://www.abcam.co.jp/ps/products/109/ab109200/Images/ab109200-7-benefits-of-recombinant-antibodies.png)
Anti-Transglutaminase 2 antibody [EP2957] (ab109200)
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (10)
ab109200 は 10 報の論文で使用されています。
- Sun W et al. The NEAT1_2/miR-491 Axis Modulates Papillary Thyroid Cancer Invasion and Metastasis Through TGM2/NF?b/FN1 Signaling. Front Oncol 11:610547 (2021). PubMed: 33738254
- Asad S et al. Proteomics-Informed Identification of Luminal Targets For In Situ Diagnosis of Inflammatory Bowel Disease. J Pharm Sci 110:239-250 (2021). PubMed: 33159915
- Strobl MAR et al. Mix and Match: Phenotypic Coexistence as a Key Facilitator of Cancer Invasion. Bull Math Biol 82:15 (2020). PubMed: 31953602
- Damaghi M et al. Collagen production and niche engineering: A novel strategy for cancer cells to survive acidosis in DCIS and evolve. Evol Appl 13:2689-2703 (2020). PubMed: 33294017
- Li ZY et al. Contribution of tissue transglutaminase to the severity of hepatic fibrosis resulting from Schistosoma japonicum infection through the regulation of IL-33/ST2 expression. Parasit Vectors 12:302 (2019). PubMed: 31200771
- Melkonian AV et al. In Vivo Measurement of Redox-Regulated TG2 Activity. Methods Mol Biol 1967:263-274 (2019). PubMed: 31069776
- Shinde AV et al. Pharmacologic inhibition of the enzymatic effects of tissue transglutaminase reduces cardiac fibrosis and attenuates cardiomyocyte hypertrophy following pressure overload. J Mol Cell Cardiol 117:36-48 (2018). PubMed: 29481819
- Lei Z et al. Novel peptide GX1 inhibits angiogenesis by specifically binding to transglutaminase-2 in the tumorous endothelial cells of gastric cancer. Cell Death Dis 9:579 (2018). PubMed: 29785022
- Mižíková I et al. Targeting transglutaminase 2 partially restores extracellular matrix structure but not alveolar architecture in experimental bronchopulmonary dysplasia. FEBS J N/A:N/A (2018). PubMed: 29935061
- Chihong Z et al. Prognostic value of Transglutaminase 2 in non-small cell lung cancer patients. Oncotarget 8:45577-45584 (2017). IHC-P ; Human . PubMed: 28715877
