Application
Flow Cytometry
Sample
Rhesus monkey Cell (rhesus monkey chorioretinal endothelial cell (RF/6)
Permeabilization
Yes - 0.02% Triton X-100 with 0.1mg/mL bovine serum albumin (BSA) for 7 min
Gating Strategy
size and granularity
Specification
rhesus monkey chorioretinal endothelial cell (RF/6
Preparation
Cell harvesting/tissue preparation method: cells were detached from tissue culture plates with 0.05% Trypsin-EDTA
Sample buffer: 2mM EDTA + 0.5% BSA in PBS
Sample buffer: 2mM EDTA + 0.5% BSA in PBS
Fixation
Paraformaldehyde
Other product details
Incubation time
20 minute(s) · Temperature: 4°C · Diluent: 2mM EDTA + 0.5% BSA in PBS
Dilution
1/100
Secondary antibody
Dilution
1/100
Name
Non-Abcam antibody was used: Vector Laboratories DyLight 488 Anti-mouse IgG DI-
Host species: Mouse
Clonality: Monoclonal
Conjugation: DyLight® 488
Host species: Mouse
Clonality: Monoclonal
Conjugation: DyLight® 488
Additional data
Abcam response
Thank you for your valuable feedback and taking part in our AbTrial program. We are pleased to see the antibody has worked well in Rhesus Monkey samples as this is the first time the antibody has been tested in this species. I checked the amino acid alignment of the monkey protein with the immunogen and it is 100%.
We will be pleased to receive Abreviews and feedback from other customers who have used the antibody in rhesus monkey in order to help verify how well it works in this sample species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Aug 03 2015