Key features and details
- Mouse monoclonal [CUB 7402] to Transglutaminase 2
- Suitable for: IHC-P, WB, Flow Cyt
- Knockout validated
- Reacts with: Rabbit, Guinea pig, Dog, Human
- Isotype: IgG1
製品名Anti-Transglutaminase 2 antibody [CUB 7402]
Transglutaminase 2 一次抗体 製品一覧
製品の詳細Mouse monoclonal [CUB 7402] to Transglutaminase 2
アプリケーション適用あり: IHC-P, WB, Flow Cytmore details
種交差性交差種: Rabbit, Guinea pig, Dog, Human
Full length native protein (purified) corresponding to Transglutaminase 2. Purified from liver.
Database link: P08587
エピトープEpitope mapping for this antibody is amino acid 447-478
- IHC-P: Human breast carcinoma tissue. WB: A549 whole cell lysate; HeLa whole cell lysate; Huvec whole cell lysate
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーPreservative: 0.05% Sodium azide
Constituent: 1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab2386 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/75. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).|
Methanol fixed cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
機能Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
配列類似性Belongs to the transglutaminase superfamily. Transglutaminase family.
- Information by UniProt
- ALPHA SUBUNIT antibody
- C polypeptide antibody
- EC 220.127.116.11 antibody
All lanes : Anti-Transglutaminase 2 antibody [CUB 7402] (ab2386) at 1 µg/ml
Lane 1 : Wild-type A549 whole cell lysate
Lane 2 : TGM2 knockout A549 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : Huvec whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 77 kDa
Observed band size: 77 kDa
Lanes 1 - 4: Merged signal (red and green). Green - ab2386 observed at 77 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab2386 was shown to recognize in wild-type A549 cells as signal was lost at the expected MW in TGM2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TGM2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab2386 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemical analysis of breast carcinoma tissue labeling Transglutaminase 2 with ab2386. Tissue was fixed with paraformaldehyde; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50) for 30 minutes at 22°C.
Overlay histogram showing HCT116 cells stained with ab2386 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2386, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HCT116 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Immunohistochemical analysis of (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections) rat lung tissue labeling Transglutaminase 2 with ab2386. Tissue was fixed with formaldehyde and blocked with 1% casein in PBS for 15 minutes at room temperature; antigen retrieval was by heat mediation in a citrate buffer, pH 6. Samples were incubated with primary antibody (1/200 in TBS + BSA) for 20 minutes. ab98782 was used as the secondary antibody at a dilution of 1/400.
ab2386 は 21 報の論文で使用されています。
- Deng Z et al. Characterization of articular cartilage homeostasis and the mechanism of superior cartilage regeneration of MRL/MpJ mice. FASEB J N/A:fj201802132RR (2019). PubMed: 31042406
- Krafft U et al. Validation of survivin and HMGA2 as biomarkers for cisplatin resistance in bladder cancer. Urol Oncol N/A:N/A (2019). PubMed: 31053526
- Qin XY et al. Arachidonic acid suppresses hepatic cell growth through ROS-mediated activation of transglutaminase. FEBS Open Bio 8:1703-1710 (2018). PubMed: 30338220
- Yunes-Medina L et al. Depletion of transglutaminase 2 in neurons alters expression of extracellular matrix and signal transduction genes and compromises cell viability. Mol Cell Neurosci 86:72-80 (2018). PubMed: 29197584
- Ruvolo PP et al. Role of MSC-derived galectin 3 in the AML microenvironment. Biochim Biophys Acta 1865:959-969 (2018). PubMed: 29655803