Anti-Transglutaminase 2 抗体 (ab421)
製品の概要
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製品名Anti-Transglutaminase 2 antibody
Transglutaminase 2 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Transglutaminase 2
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由来種Rabbit
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特異性This antibody recognises transglutaminase enzyme. MW is approximately 82kDa. This antibody cross-reacts with Factor XIIIA, Keratinocyte Tgase (TG-3), and Prostate Tgase (TG-4).
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アプリケーション適用あり: WB, ELISA, IHC-P, IHC-Fr, ICC/IFmore details
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種交差性交差種: Mouse, Rat, Guinea pig, Human
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免疫原
Full length native protein (purified) (Guinea Pig).
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ポジティブ･コントロール
- IHC-P: liver sections WB: human fibroblasts (see review), guinea pig liver lysate
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーConstituent: Whole serum
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精製度Whole antiserum
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab421 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/200 - 1/5000. | |
| ELISA | 1/300. 1/300 (See Abreview). | |
| IHC-P | 1/50 - 1/500. | |
| IHC-Fr | Use at an assay dependent concentration. PubMed: 21359202 | |
| ICC/IF | 1/1000. |
ターゲット情報
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機能Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
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配列類似性Belongs to the transglutaminase superfamily. Transglutaminase family.
- Information by UniProt
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参照データベース
- Entrez Gene: 7052 Human
- Entrez Gene: 21817 Mouse
- Entrez Gene: 56083 Rat
- Omim: 190196 Human
- SwissProt: P21980 Human
- SwissProt: P21981 Mouse
- Unigene: 517033 Human
- Unigene: 330731 Mouse
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別名
- ALPHA SUBUNIT antibody
- C polypeptide antibody
- EC 2.3.2.13 antibody
see all
画像
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Western blot - Anti-Transglutaminase 2 antibody (ab421)This was submitted as part of a review by Dr O Fromigue -
Immunocytochemistry/ Immunofluorescence - Anti-Transglutaminase 2 antibody (ab421)ICC/IF image of ab421 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab421, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunohistochemistry (Frozen sections) - Anti-Transglutaminase 2 antibody (ab421)Image from Dafik L et al., PLoS One. 2012;7(2):e30642. Epub 2012 Feb 2.; Fig 1.; doi:10.1371/journal.pone.0030642; February 2, 2012, PLoS ONE 7(2): e30642.Immnohistochemical analysis of murine intestine tissue treated with poly(I:C), staining Transglutaminase 2 with ab421.
Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 for 10 min at RT, then blocked in 0.3% Triton X-100 and 5% goat serum in PBS. Samples were incubated with primary antibody (1/250) overnight at 4°C. An AlexaFluor®488-conjugated anti-rabbit IgG was used as the secondary antibody.
プロトコール
データシートおよび資料
参考文献
This product has been referenced in:
- Dib A et al. In Utero Exposure to Maternal Diabetes Is Associated With Early Abnormal Vascular Structure in Offspring. Front Physiol 9:350 (2018). Read more (PubMed: 29670546) »
- Kuwahara G et al. CD44 Promotes Inflammation and Extracellular Matrix Production During Arteriovenous Fistula Maturation. Arterioscler Thromb Vasc Biol 37:1147-1156 (2017). Read more (PubMed: 28450292) »
