Anti-Transglutaminase 2 抗体 (ab421)

Reviews (1)Q&A (5)References (22)

Key features and details

  • Rabbit polyclonal to Transglutaminase 2
  • Suitable for: WB, ELISA, IHC-P, IHC-Fr, ICC/IF
  • Reacts with: Mouse, Rat, Guinea pig, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Transglutaminase 2 antibody
    Transglutaminase 2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Transglutaminase 2

  • 由来種

    Rabbit

  • 特異性

    This antibody recognises transglutaminase enzyme. MW is approximately 82kDa. This antibody cross-reacts with Factor XIIIA, Keratinocyte Tgase (TG-3), and Prostate Tgase (TG-4).

  • アプリケーション

    適用あり: WB, ELISA, IHC-P, IHC-Fr, ICC/IFmore details

  • 種交差性

    交差種: Mouse, Rat, Guinea pig, Human

  • 免疫原

    Full length native protein (purified) (Guinea Pig).

  • ポジティブ･コントロール

    • IHC-P: liver sections WB: human fibroblasts (see review), guinea pig liver lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/200 - 1/5000.
ELISA 1/300. 1/300 (See Abreview).
IHC-P 1/50 - 1/500.
IHC-Fr Use at an assay dependent concentration. PubMed: 21359202
ICC/IF 1/1000.

ターゲット情報

  • 機能

    Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.

  • 配列類似性

    Belongs to the transglutaminase superfamily. Transglutaminase family.
  • Information by UniProt

  • 参照データベース

    • 別名

      • ALPHA SUBUNIT antibody
      • C polypeptide antibody
      • EC 2.3.2.13 antibody
      • epididymis secretory protein Li 45 antibody
      • G alpha h antibody
      • G[a]h antibody
      • Gh CLASS G ALPHA h antibody
      • GNAH antibody
      • GNAH G PROTEIN antibody
      • H POLYPEPTIDE antibody
      • HEL-S-45 antibody
      • Protein glutamine gamma glutamyltransferase 2 antibody
      • Protein-glutamine gamma-glutamyltransferase 2 antibody
      • TG 2 antibody
      • TG(C) antibody
      • TG2 antibody
      • TGase C antibody
      • TGase H antibody
      • TGase-2 antibody
      • TgaseII antibody
      • TGC antibody
      • TGC GUANINE NUCLEOTIDE BINDING PROTEIN antibody
      • TGM2 antibody
      • TGM2_HUMAN antibody
      • Tissue transglutaminase antibody
      • Transglutaminase 2 antibody
      • Transglutaminase 2 C polypeptide antibody
      • Transglutaminase C antibody
      • Transglutaminase H antibody
      • Transglutaminase-2 antibody
      • tTG antibody
      • tTGas antibody
      see all

    画像

    • Western blot - Anti-Transglutaminase 2 antibody (ab421)
      Western blot - Anti-Transglutaminase 2 antibody (ab421)This was submitted as part of a review by Dr O Fromigue
    • Immunocytochemistry/ Immunofluorescence - Anti-Transglutaminase 2 antibody (ab421)
      Immunocytochemistry/ Immunofluorescence - Anti-Transglutaminase 2 antibody (ab421)
      ICC/IF image of ab421 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab421, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Immunohistochemistry (Frozen sections) - Anti-Transglutaminase 2 antibody (ab421)
      Immunohistochemistry (Frozen sections) - Anti-Transglutaminase 2 antibody (ab421)Image from Dafik L et al., PLoS One. 2012;7(2):e30642. Epub 2012 Feb 2.; Fig 1.; doi:10.1371/journal.pone.0030642; February 2, 2012, PLoS ONE 7(2): e30642.
      Immnohistochemical analysis of murine intestine tissue treated with poly(I:C), staining Transglutaminase 2 with ab421.

      Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 for 10 min at RT, then blocked in 0.3% Triton X-100 and 5% goat serum in PBS. Samples were incubated with primary antibody (1/250) overnight at 4°C. An AlexaFluor®488-conjugated anti-rabbit IgG was used as the secondary antibody.

    参考文献

    ab421 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab421 は 22 報の論文で使用されています。

    • Yu W  et al. Lens-specific conditional knockout of Msx2 in mice leads to ocular anterior segment dysgenesis via activation of a calcium signaling pathway. Lab Invest N/A:N/A (2019). PubMed: 30683901
    • Dib A  et al. In Utero Exposure to Maternal Diabetes Is Associated With Early Abnormal Vascular Structure in Offspring. Front Physiol 9:350 (2018). PubMed: 29670546
    • Murray-Stewart T  et al. Polyamine Homeostasis in Snyder-Robinson Syndrome. Med Sci (Basel) 6:N/A (2018). PubMed: 30544565
    • Kuwahara G  et al. CD44 Promotes Inflammation and Extracellular Matrix Production During Arteriovenous Fistula Maturation. Arterioscler Thromb Vasc Biol 37:1147-1156 (2017). PubMed: 28450292
    • Pandya CD  et al. Transglutaminase 2 overexpression induces depressive-like behavior and impaired TrkB signaling in mice. Mol Psychiatry 22:745-753 (2017). IHC . PubMed: 27620841
    View all Publications for this product

    レビューと Q&A

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    1-6 of 6 Abreviews or Q&A

    Western blot abreview for Anti-Transglutaminase 2 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - other (lung)
    Specification
    lung
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Miss. OLIVIA FROMIGUE

    Verified customer

    投稿 Aug 08 2005

    Question

    All of the data links for the human protein refer to KERATINOCYTE or EPIDERMAL transglutaminase (TGM1) yet your relevance section discusses TISSUE transglutaminase. Of the proteins with transglutaminase activity, (see Exp Mol Med 31: 5-19. 1999 for instance) what was the immunogen for this Ab? We are interested in detecting via western blot either TGase 1 OR TGase 3.

    Read More
    Answer

    Thank you for your enquiry and your patience in awaiting a response. This product is raised against tissue TGM (TGM2). I have updated the data sheet to reflect this and added data base links relating to the correct target. If you have nay further questions then please do not hesitate to get back in touch with me.

    Read More

    Question

    When this antibody was tested in Western blotting, what type of lysate(s) were used? Has this antibody ever been tested with human squamous epithelial samples?

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    Answer

    This antibody was tested in WB with lysates from guinea pig liver. It has never specifically been tested with human squamous epithelial samples. Ab421 does cross-react with Human and it is recommended for use at a dilution of 1/200 - 1/5000.

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    Question

    Customer would like to know: Has this anitbody been tested fro cross-reactivity with other isoforms?

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    Answer

    Ab421 was raised using guinea pig tissue Tgase (full length native protein (purified)). This antibody cross-reacts with Factor XIIIA, Keratinocyte Tgase (TG-3), and Prostate Tgase (TG-3). If you have any more questions please contact us again.

    Read More

    Question

    Could I know if Transglutaminase antibody (ab421) is useful on frozen section?

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    Answer

    This antibody has been tested and characterized on formalin-fixed paraffin-embedded liver tissue. We do not have data on frozen sections, however it is likely that this product will work on acetone or ethanol-fixed tissue section as well.

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    Question

    Is this tissue transglutaminase or keratinocyte TGTASE I?

    Read More
    Answer

    This antibody recognises cellular/tissue transglutaminase. The immunogen used was purified transglutaminase from guinea pig liver. 'TG1' referred to this being the first transglutaminase antibody to be added to our catalog, not the isoform of the antigen - it is ambiguous, sorry for the confusion and we have now amended the antibody name.

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