Anti-Transglutaminase 2 抗体 (ab421)
Key features and details
- Rabbit polyclonal to Transglutaminase 2
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Transglutaminase 2 antibody
Transglutaminase 2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Transglutaminase 2 -
由来種
Rabbit -
特異性
This antibody recognises transglutaminase enzyme. MW is approximately 82kDa. This antibody cross-reacts with Factor XIIIA, Keratinocyte Tgase (TG-3), and Prostate Tgase (TG-4). -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Full length native protein (purified) (Guinea Pig).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituent: Whole serum -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab421の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/1000.
|
| 特記事項 |
|---|
|
ICC/IF
1/1000. |
ターゲット情報
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機能
Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. -
配列類似性
Belongs to the transglutaminase superfamily. Transglutaminase family. - Information by UniProt
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参照データベース
- Entrez Gene: 7052 Human
- Omim: 190196 Human
- SwissProt: P21980 Human
- Unigene: 517033 Human
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別名
- ALPHA SUBUNIT antibody
- C polypeptide antibody
- EC 2.3.2.13 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Transglutaminase 2 antibody (ab421)ICC/IF image of ab421 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab421, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
データシートおよび資料
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Datasheet download
参考文献 (25)
ab421 は 25 報の論文で使用されています。
- Sheftel CM & Hernandez LL Serotonin stimulated parathyroid hormone related protein induction in the mammary epithelia by transglutaminase-dependent serotonylation. PLoS One 15:e0241192 (2020). PubMed: 33095824
- Yang P et al. TGM2 interference regulates the angiogenesis and apoptosis of colorectal cancer via Wnt/ß-catenin pathway. Cell Cycle 18:1122-1134 (2019). PubMed: 31010374
- Yu W et al. Lens-specific conditional knockout of Msx2 in mice leads to ocular anterior segment dysgenesis via activation of a calcium signaling pathway. Lab Invest N/A:N/A (2019). PubMed: 30683901
- Dib A et al. In Utero Exposure to Maternal Diabetes Is Associated With Early Abnormal Vascular Structure in Offspring. Front Physiol 9:350 (2018). PubMed: 29670546
- Murray-Stewart T et al. Polyamine Homeostasis in Snyder-Robinson Syndrome. Med Sci (Basel) 6:N/A (2018). PubMed: 30544565
