Anti-Transglutaminase 2 抗体 (ab421)

Reviews (1)Q&A (5)References (24)
Immunocytochemistry/ Immunofluorescence - Anti-Transglutaminase 2 antibody (ab421)

    Key features and details

    • Rabbit polyclonal to Transglutaminase 2
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-Transglutaminase 2 antibody
      Transglutaminase 2 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to Transglutaminase 2

    • 由来種

      Rabbit

    • 特異性

      This antibody recognises transglutaminase enzyme. MW is approximately 82kDa. This antibody cross-reacts with Factor XIIIA, Keratinocyte Tgase (TG-3), and Prostate Tgase (TG-4).

    • アプリケーション

      適用あり: ICC/IFmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Full length native protein (purified) (Guinea Pig).

    • 特記事項

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab421 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    ICC/IF 1/1000.

    ターゲット情報

    • 機能

      Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.

    • 配列類似性

      Belongs to the transglutaminase superfamily. Transglutaminase family.
    • Information by UniProt

    • 参照データベース

      • 別名

        • ALPHA SUBUNIT antibody
        • C polypeptide antibody
        • EC 2.3.2.13 antibody
        • epididymis secretory protein Li 45 antibody
        • G alpha h antibody
        • G[a]h antibody
        • Gh CLASS G ALPHA h antibody
        • GNAH antibody
        • GNAH G PROTEIN antibody
        • H POLYPEPTIDE antibody
        • HEL-S-45 antibody
        • Protein glutamine gamma glutamyltransferase 2 antibody
        • Protein-glutamine gamma-glutamyltransferase 2 antibody
        • TG 2 antibody
        • TG(C) antibody
        • TG2 antibody
        • TGase C antibody
        • TGase H antibody
        • TGase-2 antibody
        • TgaseII antibody
        • TGC antibody
        • TGC GUANINE NUCLEOTIDE BINDING PROTEIN antibody
        • TGM2 antibody
        • TGM2_HUMAN antibody
        • Tissue transglutaminase antibody
        • Transglutaminase 2 antibody
        • Transglutaminase 2 C polypeptide antibody
        • Transglutaminase C antibody
        • Transglutaminase H antibody
        • Transglutaminase-2 antibody
        • tTG antibody
        • tTGas antibody
        see all

      画像

      • Immunocytochemistry/ Immunofluorescence - Anti-Transglutaminase 2 antibody (ab421)
        Immunocytochemistry/ Immunofluorescence - Anti-Transglutaminase 2 antibody (ab421)
        ICC/IF image of ab421 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab421, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

      参考文献 (24)

      ab421 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab421 は 24 報の論文で使用されています。

      • Yu W  et al. Lens-specific conditional knockout of Msx2 in mice leads to ocular anterior segment dysgenesis via activation of a calcium signaling pathway. Lab Invest N/A:N/A (2019). PubMed: 30683901
      • Yang P  et al. TGM2 interference regulates the angiogenesis and apoptosis of colorectal cancer via Wnt/ß-catenin pathway. Cell Cycle 18:1122-1134 (2019). PubMed: 31010374
      • Murray-Stewart T  et al. Polyamine Homeostasis in Snyder-Robinson Syndrome. Med Sci (Basel) 6:N/A (2018). PubMed: 30544565
      • Png E  et al. Mechanistic role of transglutaminase-2 in focal adhesions. Sci Rep 8:12370 (2018). PubMed: 30120307
      • Dib A  et al. In Utero Exposure to Maternal Diabetes Is Associated With Early Abnormal Vascular Structure in Offspring. Front Physiol 9:350 (2018). PubMed: 29670546
      View all Publications for this product

      レビューと Q&A

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      1-6 of 6 Abreviews or Q&A

      Western blot abreview for Anti-Transglutaminase 2 antibody

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - other (lung)
      Specification
      lung
      Blocking step
      Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5%
      Read More

      Miss. OLIVIA FROMIGUE

      Verified customer

      投稿 Aug 08 2005

      Question

      All of the data links for the human protein refer to KERATINOCYTE or EPIDERMAL transglutaminase (TGM1) yet your relevance section discusses TISSUE transglutaminase. Of the proteins with transglutaminase activity, (see Exp Mol Med 31: 5-19. 1999 for instance) what was the immunogen for this Ab? We are interested in detecting via western blot either TGase 1 OR TGase 3.

      Read More
      Answer

      Thank you for your enquiry and your patience in awaiting a response. This product is raised against tissue TGM (TGM2). I have updated the data sheet to reflect this and added data base links relating to the correct target. If you have nay further questions then please do not hesitate to get back in touch with me.

      Read More

      Question

      When this antibody was tested in Western blotting, what type of lysate(s) were used? Has this antibody ever been tested with human squamous epithelial samples?

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      Answer

      This antibody was tested in WB with lysates from guinea pig liver. It has never specifically been tested with human squamous epithelial samples. Ab421 does cross-react with Human and it is recommended for use at a dilution of 1/200 - 1/5000.

      Read More

      Question

      Customer would like to know: Has this anitbody been tested fro cross-reactivity with other isoforms?

      Read More
      Answer

      Ab421 was raised using guinea pig tissue Tgase (full length native protein (purified)). This antibody cross-reacts with Factor XIIIA, Keratinocyte Tgase (TG-3), and Prostate Tgase (TG-3). If you have any more questions please contact us again.

      Read More

      Question

      Could I know if Transglutaminase antibody (ab421) is useful on frozen section?

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      Answer

      This antibody has been tested and characterized on formalin-fixed paraffin-embedded liver tissue. We do not have data on frozen sections, however it is likely that this product will work on acetone or ethanol-fixed tissue section as well.

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      Question

      Is this tissue transglutaminase or keratinocyte TGTASE I?

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      Answer

      This antibody recognises cellular/tissue transglutaminase. The immunogen used was purified transglutaminase from guinea pig liver. 'TG1' referred to this being the first transglutaminase antibody to be added to our catalog, not the isoform of the antigen - it is ambiguous, sorry for the confusion and we have now amended the antibody name.

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