Anti-Transglutaminase 2 抗体 (ab421)
製品の概要
-
製品名
Anti-Transglutaminase 2 antibody
Transglutaminase 2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Transglutaminase 2 -
由来種
Rabbit -
特異性
This antibody recognises transglutaminase enzyme. MW is approximately 82kDa. This antibody cross-reacts with Factor XIIIA, Keratinocyte Tgase (TG-3), and Prostate Tgase (TG-4). -
アプリケーション
適用あり: WB, ELISA, IHC-P, IHC-Fr, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Guinea pig, Human -
免疫原
Full length native protein (purified) (Guinea Pig).
-
ポジティブ･コントロール
- IHC-P: liver sections WB: human fibroblasts (see review), guinea pig liver lysate
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituent: Whole serum -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab421 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/200 - 1/5000. | |
| ELISA | 1/300. 1/300 (See Abreview). | |
| IHC-P | 1/50 - 1/500. | |
| IHC-Fr | Use at an assay dependent concentration. PubMed: 21359202 | |
| ICC/IF | 1/1000. |
ターゲット情報
-
機能
Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. -
配列類似性
Belongs to the transglutaminase superfamily. Transglutaminase family. - Information by UniProt
-
参照データベース
- Entrez Gene: 7052 Human
- Entrez Gene: 21817 Mouse
- Entrez Gene: 56083 Rat
- Omim: 190196 Human
- SwissProt: P21980 Human
- SwissProt: P21981 Mouse
- Unigene: 517033 Human
- Unigene: 330731 Mouse
-
別名
- ALPHA SUBUNIT antibody
- C polypeptide antibody
- EC 2.3.2.13 antibody
see all
画像
-
Western blot - Anti-Transglutaminase 2 antibody (ab421)This was submitted as part of a review by Dr O Fromigue -
Immunocytochemistry/ Immunofluorescence - Anti-Transglutaminase 2 antibody (ab421)ICC/IF image of ab421 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab421, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunohistochemistry (Frozen sections) - Anti-Transglutaminase 2 antibody (ab421)Image from Dafik L et al., PLoS One. 2012;7(2):e30642. Epub 2012 Feb 2.; Fig 1.; doi:10.1371/journal.pone.0030642; February 2, 2012, PLoS ONE 7(2): e30642.Immnohistochemical analysis of murine intestine tissue treated with poly(I:C), staining Transglutaminase 2 with ab421.
Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 for 10 min at RT, then blocked in 0.3% Triton X-100 and 5% goat serum in PBS. Samples were incubated with primary antibody (1/250) overnight at 4°C. An AlexaFluor®488-conjugated anti-rabbit IgG was used as the secondary antibody.
プロトコール
データシートおよび資料
参考文献
This product has been referenced in:
- Yu W et al. Lens-specific conditional knockout of Msx2 in mice leads to ocular anterior segment dysgenesis via activation of a calcium signaling pathway. Lab Invest N/A:N/A (2019). Read more (PubMed: 30683901) »
- Murray-Stewart T et al. Polyamine Homeostasis in Snyder-Robinson Syndrome. Med Sci (Basel) 6:N/A (2018). Read more (PubMed: 30544565) »
