Anti-Transglutaminase 2 抗体 (ab137378)
Key features and details
- Rabbit polyclonal to Transglutaminase 2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-Transglutaminase 2 antibody
Transglutaminase 2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Transglutaminase 2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Cow -
免疫原
Recombinant fragment, corresponding to a region within internal sequence amino acids 256 and 584 of Human Transglutaminase 2
-
ポジティブ・コントロール
- H1299 and HepG2 whole cell lysates; HeLa cells; H441 xenograft tissue.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab137378の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 77 kDa.
|
|
ICC/IF |
1/100 - 1/1000.
|
|
IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) may be used.
|
特記事項 |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 77 kDa. |
ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) may be used. |
ターゲット情報
-
機能
Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. -
配列類似性
Belongs to the transglutaminase superfamily. Transglutaminase family. - Information by UniProt
-
参照データベース
- Entrez Gene: 281528 Cow
- Entrez Gene: 7052 Human
- Omim: 190196 Human
- SwissProt: P51176 Cow
- SwissProt: P21980 Human
- Unigene: 517033 Human
-
別名
- ALPHA SUBUNIT antibody
- C polypeptide antibody
- EC 2.3.2.13 antibody
see all
画像
-
Anti-Transglutaminase 2 antibody (ab137378) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 77 kDa
7.5% SDS PAGE -
Immunofluorescence of paraformaldehyde-fixed HeLa cells labelling Transglutaminase 2 using ab137378 at 1/200 dilution (green). Lower panel is merged with DNA probe (blue)
-
Immunohistochemical analysis of paraffin embedded H441 xenograft tissue labelling Transglutaminase 2 using ab137378 at 1/500 dilution
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab137378 は 1 報の論文で使用されています。
- Wei W et al. Circular RNA circ_0081001 knockdown enhances methotrexate sensitivity in osteosarcoma cells by regulating miR-494-3p/TGM2 axis. J Orthop Surg Res 16:50 (2021). PubMed: 33435987