Anti-Transferrin Receptor 抗体 [MEM-189] (ab1086)

Thank you for contacting us.

According to our datasheet, this antibody only works under non-reducing conditions, I am afraid: it may be that the antibody cannot detect the reduced protein.Otherwise, it should workwith the use of your usual protocol as we do not have any other restrictions on the datasheet. As a side note, we usually use 3%BSA as a blocking agent as we find that it gives a significant stronger signal with monoclonal antibodies.

According to SwissProt, this protein has a molecular weight of 85 kDa when unmodified. However, as it has many glycosylation sides, I would expect it to run higher; the glycosylation status will influence the weight.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

The epitope of ab1086 has not been mapped. We only know that this antibody recognise an extracellular part of Transferrin Receptor.

Thank you for contacting us.

Yes, the extra blocking step is always required to avoid the mouse back ground. We have recommendations that can be found by clicking the following link;

https://www.abcam.com/index.html?pageconfig=resource&rid=11465

We have kit which is perfect for mouse on mouse staining; please have a look at the datasheet

https://www.abcam.com/Mouse-on-Mouse-MOM-Polymer-IHC-Kit-ab127055.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Thank you for your enquiry.

We have not tested these products (ab1086, ab84036, ab9179, ab8598) for cross reactivity with Trf2. However, based on immunogen sequence (ClustalW analysis, Score 23.0), we do not expect to see any cross reactivity whatsoever.

I hope this information will help settle any concerns that you may have.

Thank you for bringing that to my attention.
I did some additional research into CD71. CD71, also known as the transferrin receptor (TFR), is a type II membrane glycoprotein that exists as a disulfide-linked homodimer of two identical subunits. Ab1086 used under non-reducing conditions should recognize the homodimer at greater than 170kDa after post translation modifications. Did you see any bands in that range?
If used in reducing conditions it seems that the product should pick up the band around 85kDa (before PTM). The datasheet is not very clear about this. When repeating your experiments I would recommend looking for the larger weight. It may also be useful to try reducing conditions as well.
I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

Thank you for your response.

I am very sorry to say this but we cannot accept returned items that have been frozen. This is because we need to limit any freeze/thaw cycles that the product might experience. I am sorry about the inconvenience that this might cause.

May I suggest however that you repeat your experiment using one of these vials? I have had the opportunity to review your protocol, which looks fine. If your results are the same (a band at less than 1/2 the expected weight) then we might conclude that the issue is with the product. In which case I might refund or replace all the products without need for return.

Please let me know if this is acceptable for you and if you have any questions.

I am sorry that you have been experiencing problems with one of our products and wish to thank you for bringing this to our attention. As this order was placed and delivered within the last seven day I may issue a return for you. Since this is ultimately a problem with one of our product I am waiving the standard 20% restocking fee for you.

Before I may process this return however I will need a little information:

Your order was for three vials of ab1086. Have any of these been opened?

How have these products been stored since delivery?

I would also like to discuss the original vial of ab1086 with you and perhaps issue a free of charge replacement or refund for that product. In order to do this I will need some information about your experiments with this product. Could you please send me the protocol used?

Would you also be able to send me the order number for the original vial?

Please let me know if you have any questions.

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from ab1086.

I would like to reassure you that In the event that a product is not functioning in the applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

I would appreciate if you could also provide an image which would help us to assess the results.

Thank you for your time and cooperation. We look forward to receiving the completed questionnaire.


Order Details
Antibody code:

Lot number:

Purchase order number
or preferably Abcam order number:

General Information
Antibody storage conditions (temperature/reconstitution etc)

Description of the problem (high background, low signal, non-specific satining etc.)
Sample (Species/Tissue/Cell Type/Cell Line etc.)


Fixation of sample (Ethanol/Methanol/Acetone/Paraformaldehyde/Other/Duration etc.)


Antigen retrieval (Enzymatic method, Heat mediated technique etc.)


Permeabilization step


Blocking conditions (Buffer/time period, Blocking agent etc.)


Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Detection method


Positive and negative controls used (please specify)


Optimization attempts (problem solving)
How many times have you tried the IHC?


Have you run a "No Primary" control?
Yes No

Do you obtain the same results every time?
Yes No


What steps have you altered?


Additional Notes


We would appreciate if you are also able to provide and image which woudl help us to assess the results