Anti-Transferrin Receptor 抗体 [MEM-189] (ab1086)

Abreviews (4)Q&A (11)Specific References (12)

製品の概要

  • 製品名
    Anti-Transferrin Receptor antibody [MEM-189]
    Transferrin Receptor 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MEM-189] to Transferrin Receptor
  • 由来種
    Mouse
  • 特異性
    The antibody MEM-189 reacts with CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes. The antibody MEM-189 does not block binding of transferrin to the receptor.
  • アプリケーション
    適用あり: IHC-FrFl, Flow Cyt, IP, WBmore details
    適用なし: ICC/IF
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Tissue, cells or virus corresponding to Human Transferrin Receptor.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FrFl Use at an assay dependent concentration.
Flow Cyt Use a concentration of 2 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Use under non reducing condition.
  • 追加情報
    Is unsuitable for ICC/IF.

    • ターゲット情報

    • 機能
      Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
      (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
    • 関連疾患
      Immunodeficiency 46
    • 配列類似性
      Belongs to the peptidase M28 family. M28B subfamily.
      Contains 1 PA (protease associated) domain.
    • 翻訳後修飾
      N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
      Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
      Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
    • 細胞内局在
      Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • 参照データベース
      see all
    • 別名
      • CD 71 antibody
      • CD71 antibody
      • CD71 antigen antibody
      • IMD46 antibody
      • OTTHUMP00000208523 antibody
      • OTTHUMP00000208524 antibody
      • OTTHUMP00000208525 antibody
      • p90 antibody
      • sTfR antibody
      • T9 antibody
      • TFR 1 antibody
      • TfR antibody
      • TfR1 antibody
      • TFR1_HUMAN antibody
      • TFRC antibody
      • TR antibody
      • Transferrin receptor (p90 CD71) antibody
      • Transferrin receptor protein 1, serum form antibody
      • Trfr antibody
      see all

    画像

    • Flow Cytometry - Anti-Transferrin Receptor antibody [MEM-189] (ab1086)
      Flow Cytometry - Anti-Transferrin Receptor antibody [MEM-189] (ab1086)This image is courtesy of an anonymous Abreview

      ab1086 staining Transferrin Receptor in Human PC-3 prostate cancer cell line by Flow Cytometry. The sample was incubated with the primary antibody (0.3µg/ml in PBS + 2% FBS) for 1 hour at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG (1/500) was used as the secondary antibody.
      Gating Strategy: Cells gated for live and singlets.

      See Abreview

    参考文献

    This product has been referenced in:
    • Wang Z  et al. Pseudolaric acid B triggers ferroptosis in glioma cells via activation of Nox4 and inhibition of xCT. Cancer Lett 428:21-33 (2018). Read more (PubMed: 29702192) »
    • Yang X  et al. Sirtuin 2 regulates cellular iron homeostasis via deacetylation of transcription factor NRF2. J Clin Invest 127:1505-1516 (2017). Read more (PubMed: 28287409) »

    See all 12 Publications for this product

    ab1086 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    15 reviews or Q&A

    10
    Votes: Highest First

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Transferrin Receptor antibody [MEM-189]

     Inconclusive
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    African green monkey Cell (Kidney)
    Permeabilization
    Yes - 0.2% triton x 100
    Specification
    Kidney
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Formaldehyde
    Read More
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    Abcam user community

    Verified customer

    投稿 Jul 27 2017

    Flow Cytometry abreview for Anti-Transferrin Receptor antibody [MEM-189]

     Excellent
    Abreviews
    Application
    Flow Cytometry
    Fixation
    None
    Permeabilization
    No
    Sample
    Human Cell (PC-3 Prostrate cancer cell line)
    Specification
    PC-3 Prostrate cancer cell line
    Gating Strategy
    Cells gated for live and singlets
    Preparation
    Cell harvesting/tissue preparation method: Cells detached with PBS-based cell dissociation buffer, collected by centrifugation and re-suspended in PBS + 2% FBS
    Sample buffer: PBS + 2% FBS
    Read More
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    Username

    Abcam user community

    Verified customer

    投稿 Nov 05 2013

    .
    I'm using Anti-Transferrin Receptor antibody [MEM-189] (ab1086) for Western blot. I tested it against mouse brain tissue inreducing conditions (I used beta mercaptoethanol as denaturing agent); run on aSDS-PAGE in SDS running buffer; transferon ...

    Read More

    Thank you for contacting us.

    According to our datasheet, this antibody only works under non-reducing conditions, I am afraid: it may be that the antibody cannot detect the reduced protein.Otherwise, it should workwith the use of your usual p...

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    Dear:

    I am a customer form China beijing.We have bought an antibody from your company, Cat:AB1086,we want to know whatsite the antibody-antigen(sample) binded?the C-terminal?the N-terminal?or the other site of the ...

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    Thank you for contacting us.

    Unfortunately the epitope of ab1086 has not been mapped. We only know that this antibody recognise an extracellular part of Transferrin Receptor.

    I am sorry for not being able to provide any useful info...

    Read More

    Customer called with issues using Anti-Transferrin Receptor antibody ab1086 in rat on FACS.

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

    Read More

    I am a interested in purchasing a primary anti-transferrin receptor antibody (ab1086) which, according to the datasheet, reacts with human, mouse and rat. However, because this antibody is raised in mouse I am worried it will react to more than my prot...

    Read More

    Thank you for contacting us.

    Yes, the extra blocking step is always required to avoid the mouse back ground. We have recommendations that can be found by clicking the following link;

    https://www.abcam.com/index.html?pageconfig=reso...

    Read More

    Would these products
    (ab1086, ab84036, ab9179, ab8598) detect Trf1 and Trf2?

    Thank you for your enquiry.

    We have not tested these products (ab1086, ab84036, ab9179, ab8598) for cross reactivity with Trf2. However, based on immunogen sequence (ClustalW analysis, Score 23.0), we do not expect to see any cross reactivit...

    Read More

    Thank you for your help. I have stored them as the product information indicated, long term in the -20. But I understand keeping the freeze thaws down. I am running some more westerns soon and will include TFRC ab1086 in the bunch. I will keep you p...

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    Thank you for bringing that to my attention.
    I did some additional research into CD71. CD71, also known as the transferrin receptor (TFR), is a type II membrane glycoprotein that exists as a disulfide-linked homodimer of two identical subunits. Ab...

    Read More

    Great to hear I can return them. They have been stored in the -20 since arrival. I am not sure what information you need about the original vial so I will give you what I have and see if you have more questions. I lysed the cells in SDS free RIPA bu...

    Read More

    Thank you for your response.

    I am very sorry to say this but we cannot accept returned items that have been frozen. This is because we need to limit any freeze/thaw cycles that the product might experience. I am sorry about the inconvenience t...

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    I recently started using the Abcam antibody AB1086 for TFRC.  I ordered one tube and used it and got a positive signal on a western blot using the non reducing conditions indicated on the data sheet provided.  I recently ordered more to use in a FACS e...

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    I am sorry that you have been experiencing problems with one of our products and wish to thank you for bringing this to our attention. As this order was placed and delivered within the last seven day I may issue a return for you. Since this is ultimate...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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