Anti-Transferrin Receptor 抗体 [MEM-189] (ab1086)

Thank you for contacting us.

According to our datasheet, this antibody only works under non-reducing conditions, I am afraid: it may be that the antibody cannot detect the reduced protein.Otherwise, it should workwith the use of your usual protocol as we do not have any other restrictions on the datasheet. As a side note, we usually use 3%BSA as a blocking agent as we find that it gives a significant stronger signal with monoclonal antibodies.

According to SwissProt, this protein has a molecular weight of 85 kDa when unmodified. However, as it has many glycosylation sides, I would expect it to run higher; the glycosylation status will influence the weight.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for contacting us.

Unfortunately the epitope of ab1086 has not been mapped. We only know that this antibody recognise an extracellular part of Transferrin Receptor.

I am sorry for not being able to provide any useful information this time. Please do not hesitate to contact us if you need any more advice or information in other aspect.

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number XXXXX. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

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Yes, the extra blocking step is always required to avoid the mouse back ground. We have recommendations that can be found by clicking the following link;

https://www.abcam.com/index.html?pageconfig=resource&rid=11465

We have kit which is perfect for mouse on mouse staining; please have a look at the datasheet

https://www.abcam.com/Mouse-on-Mouse-MOM-Polymer-IHC-Kit-ab127055.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for your enquiry.

We have not tested these products (ab1086, ab84036, ab9179, ab8598) for cross reactivity with Trf2. However, based on immunogen sequence (ClustalW analysis, Score 23.0), we do not expect to see any cross reactivity whatsoever.

I hope this information will help settle any concerns that you may have.

Thank you for bringing that to my attention.
I did some additional research into CD71. CD71, also known as the transferrin receptor (TFR), is a type II membrane glycoprotein that exists as a disulfide-linked homodimer of two identical subunits. Ab1086 used under non-reducing conditions should recognize the homodimer at greater than 170kDa after post translation modifications. Did you see any bands in that range?
If used in reducing conditions it seems that the product should pick up the band around 85kDa (before PTM). The datasheet is not very clear about this. When repeating your experiments I would recommend looking for the larger weight. It may also be useful to try reducing conditions as well.
I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

Thank you for your response.

I am very sorry to say this but we cannot accept returned items that have been frozen. This is because we need to limit any freeze/thaw cycles that the product might experience. I am sorry about the inconvenience that this might cause.

May I suggest however that you repeat your experiment using one of these vials? I have had the opportunity to review your protocol, which looks fine. If your results are the same (a band at less than 1/2 the expected weight) then we might conclude that the issue is with the product. In which case I might refund or replace all the products without need for return.

Please let me know if this is acceptable for you and if you have any questions.

I am sorry that you have been experiencing problems with one of our products and wish to thank you for bringing this to our attention. As this order was placed and delivered within the last seven day I may issue a return for you. Since this is ultimately a problem with one of our product I am waiving the standard 20% restocking fee for you.

Before I may process this return however I will need a little information:

Your order was for three vials of ab1086. Have any of these been opened?

How have these products been stored since delivery?

I would also like to discuss the original vial of ab1086 with you and perhaps issue a free of charge replacement or refund for that product. In order to do this I will need some information about your experiments with this product. Could you please send me the protocol used?

Would you also be able to send me the order number for the original vial?

Please let me know if you have any questions.