Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS and 293T cell line)
Loading amount
30 µg
Specification
U2OS and 293T cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Dilution
1/100
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk 0.1% Tween-20 in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-mouse HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
Supersignal West Dura
Exposure
7 minute(s) and 0 second(s)
Bands
Specific: 95 kDa Non-specific: multiple bands kDa
Additional data
Additional Notes
This antibody detects a band of the predicted mobility in human (1: U2OS 2: 293T) and African green monkey (3: Cos7) lysates. No specific signal is observed in lysates of Ms, Hm, or Rat origin suggesting that this antibody does not recognise rodent Transferrin receptor.
Lane Order:
1: U2OS (Hu) 2: 293T (Hu) 3: Cos-7 (AGMk) 4: CHO (Hm) 5: PC12 (Rat) 6: 3T3 (Ms)
Lane Order:
1: U2OS (Hu) 2: 293T (Hu) 3: Cos-7 (AGMk) 4: CHO (Hm) 5: PC12 (Rat) 6: 3T3 (Ms)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 02 2007