Key features and details
- Mouse monoclonal [B349 (DF1513)] to Transferrin Receptor
- Suitable for: WB, Flow Cyt, IHC-Fr, ICC/IF
- Reacts with: Human
- Isotype: IgG1
製品名Anti-Transferrin Receptor antibody [B349 (DF1513)]
Transferrin Receptor 一次抗体 製品一覧
製品の詳細Mouse monoclonal [B349 (DF1513)] to Transferrin Receptor
特異性This antibody reacts with the transferrin receptor, a 180-190 kD transmembrane glycoprotein which exists as a 95 kD homodimer with interchain disulfide bond. The specificity of these antibodies, as demonstrated by immunoprecipitation, are equivalent to OKT9, B3/25 and BerT9. This antibody reacts with many proliferating cells in both normal and neoplastic tissues. It also reacts with renal tubular epithelium, islets of Langerhans, scattered cells in the anterior pituitary, hepatocytes and most tissue macrophages.
アプリケーション適用あり: WB, Flow Cyt, IHC-Fr, ICC/IFmore details
Cell line KG1.
This antibody is an indicator of proliferation activity. It also has prognostic significance when typing tumors, such as leukemias and lymphomas.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.1% Sodium azide
Concentration information loading...
精製度Protein G purified
一次抗体 備考This antibody is an indicator of proliferation activity. It also has prognostic significance when typing tumors, such as leukemias and lymphomas.
Our Abpromise guarantee covers the use of ab8598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
機能Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
配列類似性Belongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain.
翻訳後修飾N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
細胞内局在Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- CD 71 antibody
- CD71 antibody
- CD71 antigen antibody
All lanes : Anti-Transferrin Receptor antibody [B349 (DF1513)] (ab8598) at 1/100 dilution
Lane 1 : Human U2OS cell line, whole cell lysate
Lane 2 : Human 293T cell line, whole cell lysate
Lane 3 : African Green Monkey Cos7 cell line, whole cell lysate
Lane 4 : Hamster CHO cell line, whole cell lysate
Lane 5 : Rat PC12 cell line, whole cell lysate
Lane 6 : Mouse 3T3 cell line, whole cell lysate
Lysates/proteins at 30 µg per lane.
All lanes : HRP conjugated goat anti-mouse antibody
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 7 minutes
ab8598 は 4 報の論文で使用されています。
- Lehmann M et al. Nanoscale coupling of endocytic pit growth and stability. Sci Adv 5:eaax5775 (2019). PubMed: 31807703
- Perrot G et al. LRP-1--CD44, a new cell surface complex regulating tumor cell adhesion. Mol Cell Biol 32:3293-307 (2012). PubMed: 22711991
- Ortiz-Zapater E et al. Trafficking of the human transferrin receptor in plant cells: effects of tyrphostin A23 and brefeldin A. Plant J 48:757-70 (2006). PubMed: 17059402
- Ayala P et al. The pilus and porin of Neisseria gonorrhoeae cooperatively induce Ca(2+) transients in infected epithelial cells. Cell Microbiol 7:1736-48 (2005). PubMed: 16309460