Key features and details
- Mouse monoclonal [11F5] to Transferrin Receptor
- Suitable for: ELISA, Sandwich ELISA
- Reacts with: Human
- Isotype: IgG2b
製品名Anti-Transferrin Receptor antibody [11F5]
Transferrin Receptor 一次抗体 製品一覧
製品の詳細Mouse monoclonal [11F5] to Transferrin Receptor
特異性This antibody reacts with human soluble transferrin receptor.
アプリケーション適用あり: ELISA, Sandwich ELISAmore details
Recombinant full length protein (Human).
特記事項Concentration varies from lot to lot and can be provided on request.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.1% Sodium azide
Concentration information loading...
精製度Protein A purified
特記事項（精製）Purity tested by electrophoresis.
The Abpromise guarantee
sELISA: Can be paired for ELISA with Mouse monoclonal [7F8] to Transferrin Receptor (HRP) (ab10254). Can be used as detection antibody with Mouse monoclonal [2B6] to Transferrin Receptor (ab38168) as capture antibody. Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
機能Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
配列類似性Belongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain.
翻訳後修飾N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
細胞内局在Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- CD 71 antibody
- CD71 antibody
- CD71 antigen antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab10249 は 2 報の論文で使用されています。
- Peterson RD et al. Comparison of Methods Study between a Photonic Crystal Biosensor and Certified ELISA to Measure Biomarkers of Iron Deficiency in Chronic Kidney Disease Patients. Sensors (Basel) 17:N/A (2017). PubMed: 28946680
- Peterson RD et al. Enhanced sandwich immunoassay using antibody-functionalized magnetic iron-oxide nanoparticles for extraction and detection of soluble transferrin receptor on a photonic crystal biosensor. Biosens Bioelectron 74:815-22 (2015). PubMed: 26232676