製品名Anti-Transferrin antibody - Serum Loading Control
Transferrin 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to Transferrin - Serum Loading Control
アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
交差が予測される動物種: Monkey, Orangutan
- Recombinant human Transferrin protein (ab83560) can be used as a positive control in WB. This antibody gave a positive signal in HepG2 whole cell lysate as well as the following tissue lysates: Human brain; Human liver; Human heart; Human lung; Human kidney. This antibody gave a positive result in IHC in the following FFPE tissue: Human Liver.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
精製度Immunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab88165 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 77 kDa).|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
機能Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
組織特異性Expressed by the liver and secreted in plasma.
関連疾患Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
配列類似性Belongs to the transferrin family.
Contains 2 transferrin-like domains.
- Information by UniProt
- Apotransferrin antibody
- Beta 1 metal binding globulin antibody
- Beta-1 metal-binding globulin antibody
All lanes : Anti-Transferrin antibody - Serum Loading Control (ab88165) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : Human heart tissue lysate - total protein (ab29431)
Lane 5 : Lung (Human) Tissue Lysate
Lane 6 : Human kidney tissue lysate - total protein (ab30203)
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 77 kDa
Observed band size: 83 kDa why is the actual band size different from the predicted?
Additional bands at: 68 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Human Serotransferrin precursor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
IHC image of Transferrin staining in Human Liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab88165, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ICC/IF image of ab88165 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab88165, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 5µg/ml.
ab88165 has not yet been referenced specifically in any publications.