製品の概要
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製品名Anti-Transferrin antibody [HTF-14]
Transferrin 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [HTF-14] to Transferrin
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由来種Mouse
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特異性This antibody recognizes an epitope located in the N-terminal domain of human transferrin.
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アプリケーション適用あり: IHC-P, ELISA, RIA, WB, Sandwich ELISA, ICC, IP, Functional Studiesmore details
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種交差性交差種: Rabbit, Human, Pig
非交差種: Sheep, Horse, Cow, Dog, Carp -
免疫原
Full length native protein (purified) (Pig).
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度>95% by SDS-PAGE
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特記事項（精製）Purified from ascites by sequential precipitation methods.
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ポリ/モノモノクローナル
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クローン名HTF-14
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ミエローマunknown
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アイソタイプIgG1
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軽鎖の種類unknown
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研究分野
関連製品
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Affibody® Molecule
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab769 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use a concentration of 10 µg/ml. | |
| ELISA | Use at an assay dependent concentration. | |
| RIA | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Works best under non reducing conditions. |
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| Sandwich ELISA | Use a concentration of 1 µg/ml. For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab9538 as Detection. |
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| ICC | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. | |
| Functional Studies | Use at an assay dependent concentration. Effectively blocks the transferrin-transferrin receptor interactions. |
ターゲット情報
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機能Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
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組織特異性Expressed by the liver and secreted in plasma.
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関連疾患Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
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配列類似性Belongs to the transferrin family.
Contains 2 transferrin-like domains. -
細胞内局在Secreted.
- Information by UniProt
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参照データベース
- Entrez Gene: 7018 Human
- Entrez Gene: 100009267 Rabbit
- Omim: 190000 Human
- SwissProt: P02787 Human
- SwissProt: P09571 Pig
- SwissProt: P19134 Rabbit
- Unigene: 518267 Human
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別名
- Apotransferrin antibody
- Beta 1 metal binding globulin antibody
- Beta-1 metal-binding globulin antibody
see all
画像
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![Western blot - Anti-Transferrin antibody [HTF-14] (ab769)](https://www.abcam.co.jp/ps/products/0/ab769/Images/ab769-232179-ab769wb.jpg)
Western blot - Anti-Transferrin antibody [HTF-14] (ab769)All lanes : Anti-Transferrin antibody [HTF-14] (ab769)
Lane 1 : Transferrin – 8 µl (5 µg) (reducing conditions)
Lane 2 : Transferrin – 5 µl (3 µg) (reducing conditions)
Lane 3 : Transferrin – 3 µl (1 µg) (reducing conditions)
Lane 4 : Transferrin – 3 µl (1 µg) (non reducing conditions)
Lane 5 : Transferrin – 5 µl (3 µg) (non reducing conditions)
Lane 6 : Transferrin - 8 µl (8 µg) (non reducing conditions)The sample (commercial human transferrin) (1µg/ml in 1x PBS) was mixed with 4x loading buffer (non reducing) or with Laemmli buffer (reducing) and warmed for 3 minutes.
Volume of sample:5; 3; 1 µl of sample + 3; 2; 2 µl of buffer
SDS Page: 12% AA-gel, U=180V, 90 minutes, volume of loading sample:8; 5;3 µl
Marker – 3 µl
WB: Nitrocelulose membrane (8x5.5 cm), I=35mA, 90minutes
Detection:
Blocking solution: 1xPBS/5% BSA, 4°C, overnight
Primary Ab: membrane was incubated in 5 ml DMEM/10%FTS + 10 µl HTF-14 (primary Ab) (60 minutes, room temperature)
Washing solution: 1x PBS/0.1% Tween
Secondary Ab: IRDye®800CW Goat anti-mouse IgG1-specific, dilution 1/5000, diluting solution – 5% BSA/1x PBS, incubation for 60 minutes, low temperature.
Detection: Li-COR odyssey
No reducing agent should be added to the sample buffer. The sample can be denatured and processed by common Western blotting protocols, but the disulfide bonds must not be reduced.
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![Sandwich ELISA - Anti-Transferrin antibody [HTF-14] (ab769)](https://www.abcam.co.jp/ps/products/0/ab769/Images/Transferrin-Primary-antibodies-ab769-5.jpg)
Sandwich ELISA - Anti-Transferrin antibody [HTF-14] (ab769)Standard curve for Transferrin (Analyte: ab83560); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [HTF-14] to Transferrin (ab769) at 1µg/ml and Detector Antibody Rabbit polyclonal to Transferrin (ab9538) at 0.5µg/ml.
プロトコール
参考文献
This product has been referenced in:
- Lo Giudice MC et al. In situ characterization of nanoparticle biomolecular interactions in complex biological media by flow cytometry. Nat Commun 7:13475 (2016). Flow Cyt ; Human . Read more (PubMed: 27845346) »
- Shi R et al. Identification and functional study of osteosarcoma metastasis marker genes. Oncol Lett 10:1848-1852 (2015). WB ; Human . Read more (PubMed: 26622763) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"