Anti-Transferrin 抗体 [HTF-14] (ab769)

Reviews (1)Q&A (3)References (7)

製品の概要

  • 製品名
    Anti-Transferrin antibody [HTF-14]
    Transferrin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [HTF-14] to Transferrin
  • 由来種
    Mouse
  • 特異性
    This antibody recognizes an epitope located in the N-terminal domain of human transferrin.
  • アプリケーション
    適用あり: IHC-P, ELISA, RIA, WB, Sandwich ELISA, ICC, IP, Functional Studiesmore details
  • 種交差性
    交差種: Rabbit, Human, Pig
    非交差種: Sheep, Horse, Cow, Dog, Carp
  • 免疫原

    Full length native protein (purified) (Pig).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 10 µg/ml.
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

Works best under non reducing conditions.
Sandwich ELISA Use a concentration of 1 µg/ml.

For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab9538 as Detection.
ICC Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration. Effectively blocks the transferrin-transferrin receptor interactions.

ターゲット情報

  • 機能
    Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
  • 組織特異性
    Expressed by the liver and secreted in plasma.
  • 関連疾患
    Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
  • 配列類似性
    Belongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
    • 別名
      • Apotransferrin antibody
      • Beta 1 metal binding globulin antibody
      • Beta-1 metal-binding globulin antibody
      • DKFZp781D0156 antibody
      • PRO1400 antibody
      • PRO1557 antibody
      • PRO2086 antibody
      • Serotransferrin antibody
      • Serotransferrin precursor antibody
      • Siderophilin antibody
      • TF antibody
      • TFQTL1 antibody
      • Transferin antibody
      • Transferrin antibody
      • TRFE_HUMAN antibody
      see all

    画像

    • Western blot - Anti-Transferrin antibody [HTF-14] (ab769)
      Western blot - Anti-Transferrin antibody [HTF-14] (ab769)
      All lanes : Anti-Transferrin antibody [HTF-14] (ab769)

      Lane 1 : Transferrin – 8 µl (5 µg) (reducing conditions)
      Lane 2 : Transferrin – 5 µl (3 µg) (reducing conditions)
      Lane 3 : Transferrin – 3 µl (1 µg) (reducing conditions)
      Lane 4 : Transferrin – 3 µl (1 µg) (non reducing conditions)
      Lane 5 : Transferrin – 5 µl (3 µg) (non reducing conditions)
      Lane 6 : Transferrin - 8 µl (8 µg) (non reducing conditions)


      The sample (commercial human transferrin) (1µg/ml in 1x PBS) was mixed with 4x loading buffer (non reducing) or with Laemmli buffer (reducing) and warmed for 3 minutes.

      Volume of sample:5; 3; 1 µl of sample + 3; 2; 2 µl of buffer

      SDS Page: 12% AA-gel, U=180V, 90 minutes, volume of loading sample:8; 5;3 µl

      Marker – 3 µl

      WB: Nitrocelulose membrane (8x5.5 cm), I=35mA, 90minutes

      Detection:

      Blocking solution: 1xPBS/5% BSA, 4°C, overnight

      Primary Ab: membrane was incubated in 5 ml DMEM/10%FTS + 10 µl HTF-14 (primary Ab) (60 minutes, room temperature)

      Washing solution: 1x PBS/0.1% Tween

      Secondary Ab: IRDye®800CW Goat anti-mouse IgG1-specific, dilution 1/5000, diluting solution – 5% BSA/1x PBS, incubation for 60 minutes, low temperature.

      Detection: Li-COR odyssey

      No reducing agent should be added to the sample buffer. The sample can be denatured and processed by common Western blotting protocols, but the disulfide bonds must not be reduced.

    • Sandwich ELISA - Anti-Transferrin antibody [HTF-14] (ab769)
      Sandwich ELISA - Anti-Transferrin antibody [HTF-14] (ab769)
      Standard curve for Transferrin (Analyte: ab83560); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [HTF-14] to Transferrin (ab769) at 1µg/ml and Detector Antibody Rabbit polyclonal to Transferrin (ab9538) at 0.5µg/ml.

    参考文献

    This product has been referenced in:
    • Lo Giudice MC  et al. In situ characterization of nanoparticle biomolecular interactions in complex biological media by flow cytometry. Nat Commun 7:13475 (2016). Flow Cyt ; Human . Read more (PubMed: 27845346) »
    • Shi R  et al. Identification and functional study of osteosarcoma metastasis marker genes. Oncol Lett 10:1848-1852 (2015). WB ; Human . Read more (PubMed: 26622763) »
    See all 7 Publications for this product

    ab769 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

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    1-4 of 4 Abreviews or Q&A

    Question

    We wish to create a lateral flow assay, I am shopping for antibodies against human transferrin. Please help me on following questions.  1) Antibody pairing information to generate lateral flow test for detecting human transferrin  2) If such pairing information is available, we would like to know the performance of such pair in lateral flow test, i.e. the sensitivity and specificity   (crossreactivity with transferrin from other species).  3) Positive control information to validate the antibody performance in lateral flow test.

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    Answer

    Lateral flow is not a tested application, however it is very much like sandwich ELISA so any ab pair used in sELISA should work in LF. We do offer a matched antibody for use with ab769 this is ab9538. Gold conjugation is generally used in LF. We do not carry gold conjugated anti-Transferrin, so the customer will need to conjugate one ab for use as the colored marker. 1) Ab9538 is tested and guaranteed in sELISA. For use in LT we recommend using this ab9583 as the test line and ab769 as colored particle. We do not have a gold conjugation kit however these may be found at online. Control line will be Mouse IgG1 monoclonal antibody [NCM1] - Isotype control (ab27479). You will need a detection system which will be able to read the gold particles, a flatbed scanner will likely work. 2) We do not test in LT. Ab769 is known to Reacts with: Rabbit, Human, Pig and not with Sheep, Horse, Cow, Dog or Carp. AB9538 is known to react with human and Marmoset; other species have not been tested. 3) Transferrin protein (Active) ab83560 has been tested with and is the positive control for sELISA with this pair.

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    Question

    Customer kindly contacted us regarding the molecular weight of ab116604, ab129194, ab769.

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    Answer

    Thank you for contacting Abcam.

    Since all three antibodies have IgG (or a IgG subclass) isotypes, the molecular weight of each is approximately 160kDa (23-25kDa for both light chains, and 53kDa for both heavy chains).

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    Western blot abreview for Anti-Transferrin antibody [HTF-14]

     Poor
    Abreviews
    Application
    Western blot
    Sample
    Human Tissue lysate - other (artery biopsy)
    Loading amount
    20 µg
    Specification
    artery biopsy
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
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    Abcam user community

    Verified customer

    投稿 Feb 06 2007

    Question

    Here are the additional information for which you asked : 1) Is your plasma sample from human (please specify species)? *Yes* 2) How long has ab769 been stored at 4C? The storage instructions are: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles 4) *2 weeks* 3) How did you block your membrane - please specify blocking agent (and %) and blocking time? *Milk 10%* Could you also provide the purchase order number for this product = *192677* You will also find below the history of our prehundred e-mails where all this information was dejà mentioned ! Bests regards

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    Answer

    Thank you for the requested information. I have re-examined our record and I was not able to find this information in our past correspondences with you. In regards to the purchase order number "192677", this was an order for ab7355 (Acinus antibody) placed by Patricia from the Inserm. Could you please check this information again and provide the correct PO# for ab769? Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement if the product has been purchased within 90 days. Again, I apologize for any inconveniences and I look forward to your reply.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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