Anti-Transducin alpha 抗体 (ab3504)
Key features and details
- Rabbit polyclonal to Transducin alpha
- Suitable for: WB
- Reacts with: Mouse, Sheep, Cow, Pig
- Isotype: IgG
製品の概要
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製品名
Anti-Transducin alpha antibody
GNAT1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Transducin alpha -
由来種
Rabbit -
特異性
Detects Rod Transducin alpha from bovine outer rod segments and sheep retinal extract. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Sheep, Cow, Pig
交差が予測される動物種: Rat, Dog, Human, Xenopus laevis
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免疫原
Synthetic peptide corresponding to Human Transducin alpha aa 18-31.
Sequence:KLKEDAEKDARTVK
(Peptide available asab5002) -
特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
一次抗体 備考
Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light into an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentration causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin activity is believed to be shut off by phosphorylation followed by binding of the soluble protein, arrestin. Transducin, once activated by rhodopsin, promotes the hydrolysis of cGMP by PDE. The subunit composition of transducin differs between different photoreceptor cells. Rod transducin consists of rod transducin alpha (Tr alpha), T beta, and T gamma. Cone transducin is composed of cone transducin alpha (Tc alpha), T beta and T gamma. Differential transducin subunit composition of transducin is believed to be responsible for the different light sensitivities between photoreceptive cells. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab3504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 40 kDa). |
ターゲット情報
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機能
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase. -
組織特異性
Rod. Predominantly expressed in the retina followed by the ciliary body, iris and retinal pigment epithelium. -
関連疾患
Night blindness, congenital stationary, autosomal dominant 3
Night blindness, congenital stationary, 1C -
配列類似性
Belongs to the G-alpha family. G(i/o/t/z) subfamily. -
発生段階
Expressed at approximately postnatal day 7. - Information by UniProt
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参照データベース
- Entrez Gene: 281794 Cow
- Entrez Gene: 403613 Dog
- Entrez Gene: 2779 Human
- Entrez Gene: 14685 Mouse
- Entrez Gene: 363143 Rat
- Entrez Gene: 399262 Xenopus laevis
- Omim: 139330 Human
- SwissProt: P04695 Cow
see all -
別名
- CSNBAD3 antibody
- GBT1 antibody
- GNAT1 antibody
see all
画像
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Western blot - Anti-Transducin alpha antibody (ab3504)
Western blot detection of Trabsducin alpha on sheep retinal extracts using ab3504.
データシートおよび資料
参考文献 (2)
ab3504 は 2 報の論文で使用されています。
- Taniuchi K et al. Overexpression of GalNAc-transferase GalNAc-T3 promotes pancreatic cancer cell growth. Oncogene 30:4843-54 (2011). PubMed: 21625220
- Adamus G et al. Molecular biomarkers for autoimmune retinopathies: significance of anti-transducin-alpha autoantibodies. Exp Mol Pathol 87:195-203 (2009). PubMed: 19744478
