Anti-TRAIL 抗体 (ab2056)
Key features and details
- Rabbit polyclonal to TRAIL
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-TRAIL antibody
TRAIL 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TRAIL -
由来種
Rabbit -
特異性
Human. -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human TRAIL aa 261-277 (C terminal).
Sequence:CTNEHLIDMDHEASFFGA
(Peptide available asab7867) -
ポジティブ・コントロール
- HeLa whole cell lysate.
-
特記事項
Ligand for DR4 and DR5.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
-
精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
-
Isotype control
-
Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab2056 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P | Use a concentration of 20 µg/ml. | |
WB | 1/500. Detects a band of approximately 32 kDa.Can be blocked with TRAIL peptide (ab7867). Gives a 32 kD band which represents the membrane-bound form of TRAIL. |
ターゲット情報
-
機能
Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. -
組織特異性
Widespread; most predominant in spleen, lung and prostate. -
配列類似性
Belongs to the tumor necrosis factor family. -
細胞内局在
Membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 8743 Human
- Omim: 603598 Human
- SwissProt: P50591 Human
- Unigene: 478275 Human
-
別名
- Apo 2 ligand antibody
- APO 2L antibody
- Apo-2 ligand antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human brain tissue staining TRAIL with ab2056 at 20µg/ml
-
Western Blot of human brain lysate labeling TRAIL with Anti-TRAIL antibody (ab2056) at (1) 2.5 and (2) 5 µg/ml.
-
Western blot analysis of HeLa cell lysate containing 10, 2.5, or 1 ng of recombinant protein including extracellular domain of TRAIL with anti-TRAIL at 1 µg/ml Western blot analysis of HeLa cell lysate containing 10, 2.5, or 1 ng of recombinant protein containing extracellular domain of TRAIL with anti-TRAIL at 1 µg/ml.
-
Immunohistochemistry of TRAIL in human brain tissue with TRAIL antibody at 20 µg/ml.
プロトコール
データシートおよび資料
参考文献 (3)
ab2056 は 3 報の論文で使用されています。
- Melendez ME et al. Construction and characterization of a new TRAIL soluble form, active at picomolar concentrations. Oncotarget 9:27233-27241 (2018). PubMed: 29930761
- Ludwig S et al. Suppression of Lymphocyte Functions by Plasma Exosomes Correlates with Disease Activity in Patients with Head and Neck Cancer. Clin Cancer Res 23:4843-4854 (2017). PubMed: 28400428
- Kawano Y et al. TRAIL produced from multiple myeloma cells is associated with osteolytic markers. Oncol Rep 27:39-44 (2012). PubMed: 21993926