Anti-TRAIL 抗体 (ab2056)

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製品の概要

  • 製品名

    Anti-TRAIL antibody
    TRAIL 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to TRAIL

  • 由来種

    Rabbit

  • 特異性

    Human.

  • アプリケーション

    適用あり: IHC-P, WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide corresponding to Human TRAIL aa 261-277 (C terminal).
    Sequence:

    CTNEHLIDMDHEASFFGA


    (Peptide available as ab7867)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • HeLa whole cell lysate.

  • 特記事項

    Ligand for DR4 and DR5.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 20 µg/ml.
WB 1/500. Detects a band of approximately 32 kDa.Can be blocked with TRAIL peptide (ab7867).

Gives a 32 kD band which represents the membrane-bound form of TRAIL.

ターゲット情報

  • 機能

    Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.

  • 組織特異性

    Widespread; most predominant in spleen, lung and prostate.

  • 配列類似性

    Belongs to the tumor necrosis factor family.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Apo 2 ligand antibody
      • APO 2L antibody
      • Apo-2 ligand antibody
      • Apo-2L antibody
      • APO2L antibody
      • CD253 antibody
      • CD253 antigen antibody
      • Chemokine tumor necrosis factor ligand superfamily member 10 antibody
      • Protein TRAIL antibody
      • TL2 antibody
      • TNF Related Apoptosis Inducing Ligand antibody
      • TNF related apoptosis inducing ligand TRAIL antibody
      • TNF-related apoptosis-inducing ligand antibody
      • TNF10_HUMAN antibody
      • TNFSF10 antibody
      • TRAIL antibody
      • Tumor necrosis factor (ligand) family member 10 antibody
      • Tumor Necrosis Factor (ligand) Superfamily Member 10 antibody
      • Tumor necrosis factor apoptosis inducing ligand splice variant delta antibody
      • Tumor necrosis factor ligand superfamily member 10 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAIL antibody (ab2056)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAIL antibody (ab2056)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human brain tissue staining TRAIL with ab2056 at 20µg/ml
    • Western blot - Anti-TRAIL antibody (ab2056)
      Western blot - Anti-TRAIL antibody (ab2056)

      Western Blot of human brain lysate labeling TRAIL with Anti-TRAIL antibody (ab2056) at (1) 2.5 and (2) 5 µg/ml.

    • Western blot - Anti-TRAIL antibody (ab2056)
      Western blot - Anti-TRAIL antibody (ab2056)
      Western blot analysis of HeLa cell lysate containing 10, 2.5, or 1 ng of recombinant protein including extracellular domain of TRAIL with anti-TRAIL at 1 µg/ml Western blot analysis of HeLa cell lysate containing 10, 2.5, or 1 ng of recombinant protein containing extracellular domain of TRAIL with anti-TRAIL at 1 µg/ml.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAIL antibody (ab2056)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAIL antibody (ab2056)
      Immunohistochemistry of TRAIL in human brain tissue with TRAIL antibody at 20 µg/ml.

    参考文献

    ab2056 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2056 は 2 報の論文で使用されています。

    • Melendez ME  et al. Construction and characterization of a new TRAIL soluble form, active at picomolar concentrations. Oncotarget 9:27233-27241 (2018). PubMed: 29930761
    • Ludwig S  et al. Suppression of Lymphocyte Functions by Plasma Exosomes Correlates with Disease Activity in Patients with Head and Neck Cancer. Clin Cancer Res 23:4843-4854 (2017). PubMed: 28400428

    レビューと Q&A

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    1-3 of 3 Abreviews or Q&A

    Question

    Do you sell a rabbit polyclonalTRAIL antibody that recognizes an epitope between amino acid 95 and the C-terminal, that reacts with human for use in WB?

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    Answer

    Thank you for contacting us. We currently sell the following antibodies that meet your description:

    ab2435 - immunogen: C-terminal; tested species: mouse, human; tested applications: IHC-P, IHC-Fr, IP, WB, ICC/IF

    ab42121 - immunogen: aa 250 - C-terminal; tested species: human; tested applications: IHC-P, WB

    ab2056 - immunogen: aa 261 - 277; tested species: human; tested applications: IHC-P, WB

    ab46953 - immunogen: aa 133 - 144; tested species: human; tested applications: WB

    I hope this helps, please let me know if you need any additional information.

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    Question

    I was curious if you can provide me with a typical western blot image. Can you show me images againts full length human TRAIL protein? Where should I normally expect a band, 32kda or 24kda? Also, can you suggest what lysis buffer should be used for TRAIL WB, I was using the NP-40 lysis buffer, but the protein yield is very poor, what buffer do you recommend?

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    Answer

    Thank you for your enquiry. Ab2056 recognizes both the whole TRAIL protein (32 kDa) as well as its extracellular domain (19 kDa). If you are running a Western with HeLa whole cell lysate (which is recommended for a positive control) you would expect to see a band at 32 kDa. I will email you an image separately. If you are running recombinant TRAIL protein, you would see a band at 24 kDa - Recombinant human TRAIL monomer has a molecular mass of approximately 21 kDa. Due to glycosylation, recombinant human TRAIL migrates as a 24 kDa protein in SDS PAGE. The originator of this antibody used the following for the lysis buffer: HeLa cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol. If you have any additional questions, please contact us again.

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    Question

    Does this antibody recognises native Human trail in reducing gels?

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    Answer

    Yes, the antibody does recognise native human TRAIL in reducing and non-reducing gels.

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