Anti-TRAIL 抗体 (ab2056)

Submit a review Q&A (3)References (3)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAIL antibody (ab2056)
  • Western blot - Anti-TRAIL antibody (ab2056)
  • Western blot - Anti-TRAIL antibody (ab2056)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAIL antibody (ab2056)

Key features and details

  • Rabbit polyclonal to TRAIL
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-TRAIL antibody
    TRAIL 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to TRAIL

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide corresponding to Human TRAIL aa 261-277 (C terminal).
    Sequence:

    CTNEHLIDMDHEASFFGA


    (Peptide available as ab7867)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • HeLa whole cell lysate.

  • 特記事項

    Ligand for DR4 and DR5.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab2056の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use a concentration of 20 µg/ml.
WB
1/500. Detects a band of approximately 32 kDa.Can be blocked with TRAIL peptide (ab7867).

Gives a 32 kD band which represents the membrane-bound form of TRAIL.
特記事項
IHC-P
Use a concentration of 20 µg/ml.
WB
1/500. Detects a band of approximately 32 kDa.Can be blocked with TRAIL peptide (ab7867).

Gives a 32 kD band which represents the membrane-bound form of TRAIL.

ターゲット情報

  • 機能

    Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.

  • 組織特異性

    Widespread; most predominant in spleen, lung and prostate.

  • 配列類似性

    Belongs to the tumor necrosis factor family.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Apo 2 ligand antibody
      • APO 2L antibody
      • Apo-2 ligand antibody
      • Apo-2L antibody
      • APO2L antibody
      • CD253 antibody
      • CD253 antigen antibody
      • Chemokine tumor necrosis factor ligand superfamily member 10 antibody
      • Protein TRAIL antibody
      • TL2 antibody
      • TNF Related Apoptosis Inducing Ligand antibody
      • TNF related apoptosis inducing ligand TRAIL antibody
      • TNF-related apoptosis-inducing ligand antibody
      • TNF10_HUMAN antibody
      • TNFSF10 antibody
      • TRAIL antibody
      • Tumor necrosis factor (ligand) family member 10 antibody
      • Tumor Necrosis Factor (ligand) Superfamily Member 10 antibody
      • Tumor necrosis factor apoptosis inducing ligand splice variant delta antibody
      • Tumor necrosis factor ligand superfamily member 10 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAIL antibody (ab2056)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAIL antibody (ab2056)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human brain tissue staining TRAIL with ab2056 at 20µg/ml
    • Western blot - Anti-TRAIL antibody (ab2056)
      Western blot - Anti-TRAIL antibody (ab2056)

      Western Blot of human brain lysate labeling TRAIL with Anti-TRAIL antibody (ab2056) at (1) 2.5 and (2) 5 µg/ml.

    • Western blot - Anti-TRAIL antibody (ab2056)
      Western blot - Anti-TRAIL antibody (ab2056)
      Western blot analysis of HeLa cell lysate containing 10, 2.5, or 1 ng of recombinant protein including extracellular domain of TRAIL with anti-TRAIL at 1 µg/ml Western blot analysis of HeLa cell lysate containing 10, 2.5, or 1 ng of recombinant protein containing extracellular domain of TRAIL with anti-TRAIL at 1 µg/ml.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAIL antibody (ab2056)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAIL antibody (ab2056)
      Immunohistochemistry of TRAIL in human brain tissue with TRAIL antibody at 20 µg/ml.

    参考文献 (3)

    ab2056 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2056 は 3 報の論文で使用されています。

    • Melendez ME  et al. Construction and characterization of a new TRAIL soluble form, active at picomolar concentrations. Oncotarget 9:27233-27241 (2018). PubMed: 29930761
    • Ludwig S  et al. Suppression of Lymphocyte Functions by Plasma Exosomes Correlates with Disease Activity in Patients with Head and Neck Cancer. Clin Cancer Res 23:4843-4854 (2017). PubMed: 28400428
    • Kawano Y  et al. TRAIL produced from multiple myeloma cells is associated with osteolytic markers. Oncol Rep 27:39-44 (2012). PubMed: 21993926

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-3 of 3 Abreviews or Q&A

    Question

    Do you sell a rabbit polyclonalTRAIL antibody that recognizes an epitope between amino acid 95 and the C-terminal, that reacts with human for use in WB?

    Read More
    Answer

    Thank you for contacting us. We currently sell the following antibodies that meet your description:

    ab2435 - immunogen: C-terminal; tested species: mouse, human; tested applications: IHC-P, IHC-Fr, IP, WB, ICC/IF

    ab42121 - immunogen: aa 250 - C-terminal; tested species: human; tested applications: IHC-P, WB

    ab2056 - immunogen: aa 261 - 277; tested species: human; tested applications: IHC-P, WB

    ab46953 - immunogen: aa 133 - 144; tested species: human; tested applications: WB

    I hope this helps, please let me know if you need any additional information.

    Read More

    Question

    I was curious if you can provide me with a typical western blot image. Can you show me images againts full length human TRAIL protein? Where should I normally expect a band, 32kda or 24kda? Also, can you suggest what lysis buffer should be used for TRAIL WB, I was using the NP-40 lysis buffer, but the protein yield is very poor, what buffer do you recommend?

    Read More
    Answer

    Thank you for your enquiry. Ab2056 recognizes both the whole TRAIL protein (32 kDa) as well as its extracellular domain (19 kDa). If you are running a Western with HeLa whole cell lysate (which is recommended for a positive control) you would expect to see a band at 32 kDa. I will email you an image separately. If you are running recombinant TRAIL protein, you would see a band at 24 kDa - Recombinant human TRAIL monomer has a molecular mass of approximately 21 kDa. Due to glycosylation, recombinant human TRAIL migrates as a 24 kDa protein in SDS PAGE. The originator of this antibody used the following for the lysis buffer: HeLa cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol. If you have any additional questions, please contact us again.

    Read More

    Question

    Does this antibody recognises native Human trail in reducing gels?

    Read More
    Answer

    Yes, the antibody does recognise native human TRAIL in reducing and non-reducing gels.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com