製品の概要

  • 製品名

  • 製品の詳細

    Rabbit polyclonal to TRAIL
  • 由来種

    Rabbit
  • 特異性

    Human.
  • アプリケーション

    適用あり: IHC-P, WBmore details
  • 種交差性

    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human TRAIL aa 261-277 (C terminal).
    Sequence:

    CTNEHLIDMDHEASFFGA


    (Peptide available as ab7867)

  • ポジティブ・コントロール

    • HeLa whole cell lysate.
  • 特記事項

    Ligand for DR4 and DR5.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 20 µg/ml.
WB 1/500. Detects a band of approximately 32 kDa.Can be blocked with TRAIL peptide (ab7867).

Gives a 32 kD band which represents the membrane-bound form of TRAIL.

ターゲット情報

  • 機能

    Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.
  • 組織特異性

    Widespread; most predominant in spleen, lung and prostate.
  • 配列類似性

    Belongs to the tumor necrosis factor family.
  • 細胞内局在

    Membrane.
  • Information by UniProt
  • 参照データベース

  • 別名

    • Apo 2 ligand antibody
    • APO 2L antibody
    • Apo-2 ligand antibody
    • Apo-2L antibody
    • APO2L antibody
    • CD253 antibody
    • CD253 antigen antibody
    • Chemokine tumor necrosis factor ligand superfamily member 10 antibody
    • Protein TRAIL antibody
    • TL2 antibody
    • TNF Related Apoptosis Inducing Ligand antibody
    • TNF related apoptosis inducing ligand TRAIL antibody
    • TNF-related apoptosis-inducing ligand antibody
    • TNF10_HUMAN antibody
    • TNFSF10 antibody
    • TRAIL antibody
    • Tumor necrosis factor (ligand) family member 10 antibody
    • Tumor Necrosis Factor (ligand) Superfamily Member 10 antibody
    • Tumor necrosis factor apoptosis inducing ligand splice variant delta antibody
    • Tumor necrosis factor ligand superfamily member 10 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human brain tissue staining TRAIL with ab2056 at 20µg/ml
  • Western Blot of human brain lysate labeling TRAIL with Anti-TRAIL antibody (ab2056) at (1) 2.5 and (2) 5 µg/ml.
  • Western blot analysis of HeLa cell lysate containing 10, 2.5, or 1 ng of recombinant protein including extracellular domain of TRAIL with anti-TRAIL at 1 µg/ml Western blot analysis of HeLa cell lysate containing 10, 2.5, or 1 ng of recombinant protein containing extracellular domain of TRAIL with anti-TRAIL at 1 µg/ml.
  • Immunohistochemistry of TRAIL in human brain tissue with TRAIL antibody at 20 µg/ml.

参考文献

This product has been referenced in:

  • Melendez ME  et al. Construction and characterization of a new TRAIL soluble form, active at picomolar concentrations. Oncotarget 9:27233-27241 (2018). Read more (PubMed: 29930761) »
  • Ludwig S  et al. Suppression of Lymphocyte Functions by Plasma Exosomes Correlates with Disease Activity in Patients with Head and Neck Cancer. Clin Cancer Res 23:4843-4854 (2017). Read more (PubMed: 28400428) »
See all 2 Publications for this product

レビューと Q&A

1-3 of 3 Abreviews or Q&A

Answer

Thank you for contacting us. We currently sell the following antibodies that meet your description:

ab2435 - immunogen: C-terminal; tested species: mouse, human; tested applications: IHC-P, IHC-Fr, IP, WB, ICC/IF

ab42121 - immunogen: aa 250 - C-terminal; tested species: human; tested applications: IHC-P, WB

ab2056 - immunogen: aa 261 - 277; tested species: human; tested applications: IHC-P, WB

ab46953 - immunogen: aa 133 - 144; tested species: human; tested applications: WB

I hope this helps, please let me know if you need any additional information.

Read More

Answer

Thank you for your enquiry. Ab2056 recognizes both the whole TRAIL protein (32 kDa) as well as its extracellular domain (19 kDa). If you are running a Western with HeLa whole cell lysate (which is recommended for a positive control) you would expect to see a band at 32 kDa. I will email you an image separately. If you are running recombinant TRAIL protein, you would see a band at 24 kDa - Recombinant human TRAIL monomer has a molecular mass of approximately 21 kDa. Due to glycosylation, recombinant human TRAIL migrates as a 24 kDa protein in SDS PAGE. The originator of this antibody used the following for the lysis buffer: HeLa cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol. If you have any additional questions, please contact us again.

Read More

Question
Answer

Yes, the antibody does recognise native human TRAIL in reducing and non-reducing gels.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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