Anti-TRAIL 抗体 [2E5] (ab2219)

Submit a review Q&A (2)References (5)

製品の概要

  • 製品名

    Anti-TRAIL antibody [2E5]
    TRAIL 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [2E5] to TRAIL

  • 由来種

    Mouse

  • 特異性

    This antibody is specific for TRAIL, Tumour necrosis factor (TNF) related apoptosis inducing ligand (TRAIL/ APO2-L)).

  • アプリケーション

    適用あり: Functional Studies, Flow Cyt, Neutralisingmore details

  • 種交差性

    交差種: Human
    非交差種: Mouse

  • 免疫原

    Recombinant soluble fragment, corresponding to amino acids 95-281 of Human TRAIL.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration. The antibody 2E5 has high neutralizing activity for human TRAIL in biological assays.
Flow Cyt Use a concentration of 1 - 10 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Neutralising Use at an assay dependent concentration. The antibody 2E5 has high neutralizing activity for human TRAIL in biological assays.

ターゲット情報

  • 機能

    Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.

  • 組織特異性

    Widespread; most predominant in spleen, lung and prostate.

  • 配列類似性

    Belongs to the tumor necrosis factor family.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Apo 2 ligand antibody
      • APO 2L antibody
      • Apo-2 ligand antibody
      • Apo-2L antibody
      • APO2L antibody
      • CD253 antibody
      • CD253 antigen antibody
      • Chemokine tumor necrosis factor ligand superfamily member 10 antibody
      • Protein TRAIL antibody
      • TL2 antibody
      • TNF Related Apoptosis Inducing Ligand antibody
      • TNF related apoptosis inducing ligand TRAIL antibody
      • TNF-related apoptosis-inducing ligand antibody
      • TNF10_HUMAN antibody
      • TNFSF10 antibody
      • TRAIL antibody
      • Tumor necrosis factor (ligand) family member 10 antibody
      • Tumor Necrosis Factor (ligand) Superfamily Member 10 antibody
      • Tumor necrosis factor apoptosis inducing ligand splice variant delta antibody
      • Tumor necrosis factor ligand superfamily member 10 antibody
      see all

    画像

    • Functional Studies - Anti-TRAIL antibody [2E5] (ab2219)
      Functional Studies - Anti-TRAIL antibody [2E5] (ab2219)
      Apoptosis induced in JURKAT human T cell leukemia cell line by soluble recombinant human TRAIL is completely blocked by anti-human TRAIL (2E5).
      The neutralizing activity of the antibody 2E5 has been confirmed with various sources of soluble recombinant human TRAIL.
      A - medium
      B - recombinant TRAIL
      C - recombinant TRAIL + anti-human TRAIL (2E5; 0.06 µg/ml)
      D - recombinant TRAIL + anti-human TRAIL (2E5; 0.24 µg/ml)
      E - recombinant TRAIL + Isotype mouse IgG1 control

    参考文献

    ab2219 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2219 は 5 報の論文で使用されています。

    • Tatzel K  et al. Membrane-proximal TRAIL species are incapable of inducing short circuit apoptosis signaling: Implications for drug development and basic cytokine biology. Sci Rep 6:22661 (2016). Flow Cyt . PubMed: 26935795
    • Yunker PJ  et al. One-pot system for synthesis, assembly, and display of functional single-span membrane proteins on oil-water interfaces. Proc Natl Acad Sci U S A 113:608-13 (2016). PubMed: 26721399
    • Sharova TY  et al. Complex Changes in the Apoptotic and Cell Differentiation Programs during Initiation of the Hair Follicle Response to Chemotherapy. J Invest Dermatol N/A:N/A (2014). PubMed: 24999588
    • Hyer ML  et al. Synthetic triterpenoids cooperate with tumor necrosis factor-related apoptosis-inducing ligand to induce apoptosis of breast cancer cells. Cancer Res 65:4799-808 (2005). PubMed: 15930300
    • Plasilova M  et al. TRAIL (Apo2L) suppresses growth of primary human leukemia and myelodysplasia progenitors. Leukemia 16:67-73 (2002). PubMed: 11840265

    レビューと Q&A

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    Question

    I would like to know if this antibody is an agonist vs antagonist of TRAIL receptor. You mentioned functional studies on the datasheet. Many thanks,

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    Answer

    This antibody was raised against soluble recombinant TRAIL /APO-2L (extracellular domain). It completely neutralizes apoptosis in cells induced by TRAIL (e.g. by soluble recombinant human TRAIL) that is why datasheet states functional application.

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    Question

    send functional study data for ab2219

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    Answer

    Thank you for contacting us.
    I have attached the data for the Functional Studies done with ab2219. We will add these to the datasheet as well very soon.
    Also, I wanted to send you the information about our 3 for 2 promotion for all proteins that is going on until the end of the month.
    https://www.abcam.com/index.html?pageconfig=resource&rid=15079
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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