Anti-TRAIL 抗体 [2E5] (ab2219)

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Functional Studies - Anti-TRAIL antibody [2E5] (ab2219)

    Key features and details

    • Mouse monoclonal [2E5] to TRAIL
    • Suitable for: Functional Studies
    • Reacts with: Human
    • Isotype: IgG1

    製品の概要

    • 製品名

      Anti-TRAIL antibody [2E5]
      TRAIL 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [2E5] to TRAIL

    • 由来種

      Mouse

    • 特異性

      This antibody is specific for TRAIL, Tumour necrosis factor (TNF) related apoptosis inducing ligand (TRAIL/ APO2-L)).

    • アプリケーション

      適用あり: Functional Studiesmore details

    • 種交差性

      交差種: Human
      非交差種: Mouse

    • 免疫原

      Recombinant soluble fragment, corresponding to amino acids 95-281 of Human TRAIL.

    • 特記事項

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab2219 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Functional Studies Use at an assay dependent concentration. The antibody 2E5 has high neutralizing activity for human TRAIL in biological assays.

    ターゲット情報

    • 機能

      Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.

    • 組織特異性

      Widespread; most predominant in spleen, lung and prostate.

    • 配列類似性

      Belongs to the tumor necrosis factor family.

    • 細胞内局在

      Membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Apo 2 ligand antibody
        • APO 2L antibody
        • Apo-2 ligand antibody
        • Apo-2L antibody
        • APO2L antibody
        • CD253 antibody
        • CD253 antigen antibody
        • Chemokine tumor necrosis factor ligand superfamily member 10 antibody
        • Protein TRAIL antibody
        • TL2 antibody
        • TNF Related Apoptosis Inducing Ligand antibody
        • TNF related apoptosis inducing ligand TRAIL antibody
        • TNF-related apoptosis-inducing ligand antibody
        • TNF10_HUMAN antibody
        • TNFSF10 antibody
        • TRAIL antibody
        • Tumor necrosis factor (ligand) family member 10 antibody
        • Tumor Necrosis Factor (ligand) Superfamily Member 10 antibody
        • Tumor necrosis factor apoptosis inducing ligand splice variant delta antibody
        • Tumor necrosis factor ligand superfamily member 10 antibody
        see all

      画像

      • Functional Studies - Anti-TRAIL antibody [2E5] (ab2219)
        Functional Studies - Anti-TRAIL antibody [2E5] (ab2219)
        Apoptosis induced in JURKAT human T cell leukemia cell line by soluble recombinant human TRAIL is completely blocked by anti-human TRAIL (2E5).
        The neutralizing activity of the antibody 2E5 has been confirmed with various sources of soluble recombinant human TRAIL.
        A - medium
        B - recombinant TRAIL
        C - recombinant TRAIL + anti-human TRAIL (2E5; 0.06 µg/ml)
        D - recombinant TRAIL + anti-human TRAIL (2E5; 0.24 µg/ml)
        E - recombinant TRAIL + Isotype mouse IgG1 control

      参考文献 (5)

      ab2219 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab2219 は 5 報の論文で使用されています。

      • Tatzel K  et al. Membrane-proximal TRAIL species are incapable of inducing short circuit apoptosis signaling: Implications for drug development and basic cytokine biology. Sci Rep 6:22661 (2016). Flow Cyt . PubMed: 26935795
      • Yunker PJ  et al. One-pot system for synthesis, assembly, and display of functional single-span membrane proteins on oil-water interfaces. Proc Natl Acad Sci U S A 113:608-13 (2016). PubMed: 26721399
      • Sharova TY  et al. Complex Changes in the Apoptotic and Cell Differentiation Programs during Initiation of the Hair Follicle Response to Chemotherapy. J Invest Dermatol N/A:N/A (2014). PubMed: 24999588
      • Hyer ML  et al. Synthetic triterpenoids cooperate with tumor necrosis factor-related apoptosis-inducing ligand to induce apoptosis of breast cancer cells. Cancer Res 65:4799-808 (2005). PubMed: 15930300
      • Plasilova M  et al. TRAIL (Apo2L) suppresses growth of primary human leukemia and myelodysplasia progenitors. Leukemia 16:67-73 (2002). PubMed: 11840265

      レビューと Q&A

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      1-2 of 2 Abreviews or Q&A

      Question

      I would like to know if this antibody is an agonist vs antagonist of TRAIL receptor. You mentioned functional studies on the datasheet. Many thanks,

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      Answer

      This antibody was raised against soluble recombinant TRAIL /APO-2L (extracellular domain). It completely neutralizes apoptosis in cells induced by TRAIL (e.g. by soluble recombinant human TRAIL) that is why datasheet states functional application.

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      Question

      send functional study data for ab2219

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      Answer

      Thank you for contacting us.
      I have attached the data for the Functional Studies done with ab2219. We will add these to the datasheet as well very soon.
      Also, I wanted to send you the information about our 3 for 2 promotion for all proteins that is going on until the end of the month.
      https://www.abcam.com/index.html?pageconfig=resource&rid=15079
      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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