Anti-TRAIL 抗体 [2E5] (ab2219)
![Functional Studies - Anti-TRAIL antibody [2E5] (ab2219)](https://www.abcam.co.jp/ps/products/2/ab2219/Images/ab2219-5477-anti-trail-antibody-2e5.jpg "Functional Studies - Anti-TRAIL antibody [2E5] (ab2219)")
Key features and details
- Mouse monoclonal [2E5] to TRAIL
- Suitable for: Functional Studies
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
Anti-TRAIL antibody [2E5]
TRAIL 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2E5] to TRAIL -
由来種
Mouse -
特異性
This antibody is specific for TRAIL, Tumour necrosis factor (TNF) related apoptosis inducing ligand (TRAIL/ APO2-L)).
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アプリケーション
適用あり: Functional Studiesmore details -
種交差性
交差種: Human
非交差種: Mouse -
免疫原
Recombinant soluble fragment, corresponding to amino acids 95-281 of Human TRAIL.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
Purified from TCS. -
ポリ/モノ
モノクローナル -
クローン名
2E5 -
ミエローマ
unknown -
アイソタイプ
IgG1 -
軽鎖の種類
unknown -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2219の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration. The antibody 2E5 has high neutralizing activity for human TRAIL in biological assays.
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| 特記事項 |
|---|
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Functional Studies
Use at an assay dependent concentration. The antibody 2E5 has high neutralizing activity for human TRAIL in biological assays. |
ターゲット情報
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機能
Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. -
組織特異性
Widespread; most predominant in spleen, lung and prostate. -
配列類似性
Belongs to the tumor necrosis factor family. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 8743 Human
- Omim: 603598 Human
- SwissProt: P50591 Human
- Unigene: 478275 Human
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別名
- Apo 2 ligand antibody
- APO 2L antibody
- Apo-2 ligand antibody
see all
画像
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Functional Studies - Anti-TRAIL antibody [2E5] (ab2219)Apoptosis induced in JURKAT human T cell leukemia cell line by soluble recombinant human TRAIL is completely blocked by anti-human TRAIL (2E5).
The neutralizing activity of the antibody 2E5 has been confirmed with various sources of soluble recombinant human TRAIL.
A - medium
B - recombinant TRAIL
C - recombinant TRAIL + anti-human TRAIL (2E5; 0.06 µg/ml)
D - recombinant TRAIL + anti-human TRAIL (2E5; 0.24 µg/ml)
E - recombinant TRAIL + Isotype mouse IgG1 control
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab2219 は 6 報の論文で使用されています。
- Nordmaj MA et al. Development of a bispecific immune engager using a recombinant malaria protein. Cell Death Dis 12:353 (2021). PubMed: 33824272
- Tatzel K et al. Membrane-proximal TRAIL species are incapable of inducing short circuit apoptosis signaling: Implications for drug development and basic cytokine biology. Sci Rep 6:22661 (2016). Flow Cyt . PubMed: 26935795
- Yunker PJ et al. One-pot system for synthesis, assembly, and display of functional single-span membrane proteins on oil-water interfaces. Proc Natl Acad Sci U S A 113:608-13 (2016). PubMed: 26721399
- Sharova TY et al. Complex Changes in the Apoptotic and Cell Differentiation Programs during Initiation of the Hair Follicle Response to Chemotherapy. J Invest Dermatol N/A:N/A (2014). PubMed: 24999588
- Hyer ML et al. Synthetic triterpenoids cooperate with tumor necrosis factor-related apoptosis-inducing ligand to induce apoptosis of breast cancer cells. Cancer Res 65:4799-808 (2005). PubMed: 15930300
- Plasilova M et al. TRAIL (Apo2L) suppresses growth of primary human leukemia and myelodysplasia progenitors. Leukemia 16:67-73 (2002). PubMed: 11840265