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リコンビナントRabMAb

Anti-TRAF4 抗体 [EPR1729] (ab108991)

Reviews (1) Submit a question References (3)
Flow Cytometry (Intracellular) - Anti-TRAF4 antibody [EPR1729] (ab108991)
  • Western blot - Anti-TRAF4 antibody [EPR1729] (ab108991)
  • Anti-TRAF4 antibody [EPR1729] (ab108991)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1729] to TRAF4
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-TRAF4 antibody [EPR1729]
    TRAF4 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR1729] to TRAF4

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WBmore details
    適用なし: IHC-P or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Jurkat, HeLa and SKBR-3 cell lysates

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab108991の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
追加情報
Is unsuitable for IHC-P or IP.

ターゲット情報

  • 機能

    Adapter protein and signal transducer that links members of the tumor necrosis factor receptor (TNFR) family to different signaling pathways. Plays a role in the activation of NF-kappa-B and JNK, and in the regulation of cell survival and apoptosis. Regulates activation of NF-kappa-B in response to signaling through Toll-like receptors. Required for normal skeleton development, and for normal development of the respiratory tract (By similarity). Required for activation of RPS6KB1 in response to TNF signaling. Modulates TRAF6 functions.

  • 組織特異性

    Expressed in epithelial cells of thymus, dendritic cells of lymph node, and in the basal cell layer of epithelia such as epidermis, nasopharynx, respiratory tract, salivary gland, and esophagus.

  • 配列類似性

    Belongs to the TNF receptor-associated factor family. B subfamily.
    Contains 1 MATH domain.
    Contains 1 RING-type zinc finger.
    Contains 3 TRAF-type zinc fingers.

  • ドメイン

    The coiled coil domain mediates homo- and hetero-oligomerization.
    The MATH/TRAF domain binds to receptor cytoplasmic domains.

  • 翻訳後修飾

    Polyubiquitinated, leading to its proteasomal degradation.

  • 細胞内局在

    Cytoplasm. Nucleus. Cytoplasm > perinuclear region. Cell junction > tight junction. Cell membrane. Cytoplasm > cytoskeleton.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CART 1 antibody
      • CART1 antibody
      • cysteine rich domain associated with ring and TRAF domain antibody
      • Cysteine rich domain associated with RING and Traf domains protein 1 antibody
      • Cysteine-rich domain associated with RING and Traf domains protein 1 antibody
      • Malignant 62 antibody
      • Metastatic lymph node gene 62 protein antibody
      • MLN 62 antibody
      • MLN62 antibody
      • RING finger protein 83 antibody
      • RNF 83 antibody
      • RNF83 antibody
      • TNF receptor associated factor 4 antibody
      • TNF receptor-associated factor 4 antibody
      • TRAF 4 antibody
      • Traf4 antibody
      • TRAF4_HUMAN antibody
      • tumor necrosis receptor-associated factor 4A antibody
      see all

    画像

    • Flow Cytometry (Intracellular) - Anti-TRAF4 antibody [EPR1729] (ab108991)
      Flow Cytometry (Intracellular) - Anti-TRAF4 antibody [EPR1729] (ab108991)

      Overlay histogram showing HeLa cells stained with ab108991 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108991, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

       

    • Western blot - Anti-TRAF4 antibody [EPR1729] (ab108991)
      Western blot - Anti-TRAF4 antibody [EPR1729] (ab108991)
      All lanes : Anti-TRAF4 antibody [EPR1729] (ab108991) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : SKBR-3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 54 kDa

    • Anti-TRAF4 antibody [EPR1729] (ab108991)
      Anti-TRAF4 antibody [EPR1729] (ab108991)

    参考文献 (3)

    ab108991 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab108991 は 3 報の論文で使用されています。

    • Zhang Z  et al. HFD-induced TRAF6 upregulation promotes liver cholesterol accumulation and fatty liver development via EZH2-mediated miR-429/PPARa axis. Mol Ther Nucleic Acids 24:711-727 (2021). PubMed: 33996254
    • Wang J  et al. Long non-coding RNA DLEU1 promotes cell proliferation of glioblastoma multiforme. Mol Med Rep 20:1873-1882 (2019). PubMed: 31257517
    • Zepp JA  et al. Cutting edge: TNF receptor-associated factor 4 restricts IL-17-mediated pathology and signaling processes. J Immunol 189:33-7 (2012). PubMed: 22649194

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Western blot abreview for Anti-TRAF4 antibody [EPR1729]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (LnCaP cells)
    Loading amount
    50 µg
    Specification
    LnCaP cells
    Gel Running Conditions
    Reduced Denaturing (5-15% gradient gel)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    投稿 Nov 14 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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