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  1. Link

    traf2-antibody-epr6048-ab126758.pdf

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Cell Biology Apoptosis Intracellular Associated Proteins
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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-TRAF2 抗体 [EPR6048] (ab126758)

  • Datasheet
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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-TRAF2 antibody [EPR6048] (ab126758)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF2 antibody [EPR6048] (ab126758)
  • Western blot - Anti-TRAF2 antibody [EPR6048] (ab126758)
  • Flow Cytometry (Intracellular) - Anti-TRAF2 antibody [EPR6048] (ab126758)
  • Western blot - Anti-TRAF2 antibody [EPR6048] (ab126758)
  • Western blot - Anti-TRAF2 antibody [EPR6048] (ab126758)
  • Western blot - Anti-TRAF2 antibody [EPR6048] (ab126758)
  • Immunocytochemistry/ Immunofluorescence - Anti-TRAF2 antibody [EPR6048] (ab126758)
  • Immunoprecipitation - Anti-TRAF2 antibody [EPR6048] (ab126758)
  • Flow Cytometry (Intracellular) - Anti-TRAF2 antibody [EPR6048] (ab126758)
  • Western blot - Anti-TRAF2 antibody [EPR6048] (ab126758)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF2 antibody [EPR6048] (ab126758)
  • Immunocytochemistry/ Immunofluorescence - Anti-TRAF2 antibody [EPR6048] (ab126758)
  • OI-RD Scanning - Anti-TRAF2 antibody [EPR6048] (ab126758)
  • Anti-TRAF2 antibody [EPR6048] (ab126758)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6048] to TRAF2
  • Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 594 Carrier Free PE

こちらの製品もご検討ください

二次抗体
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
ノックアウト
Product image
Human TRAF2 knockout HEK-293T cell line (ab266060)

関連製品

製品の概要

  • 製品名

    Anti-TRAF2 antibody [EPR6048]
    TRAF2 一次抗体 製品一覧
  • 製品の詳細

    Rabbit monoclonal [EPR6048] to TRAF2
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IFmore details
  • 種交差性

    交差種: Rat, Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ・コントロール

    • WB: HEK-293T, PC12, MOLT4, HeLa, and 293T cell lysates. ICC: HeLa cells. IHC-P: Human kidney tissue. Flow Cyt (intra): HeLa cells.
  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • 解離定数(KD 値)

    KD = 1.70 x 10 -12 M
    Learn more about KD
  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    EPR6048
  • アイソタイプ

    IgG
  • 研究分野

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Associated Proteins
    • Signal Transduction
    • Adapters
    • Cytoplasmic
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Signal Transduction
    • Growth Factors/Hormones
    • TNF
    • Cancer
    • Growth factors
    • TNF
    • Cancer
    • Signal transduction
    • Other
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators

関連製品

  • Alternative Versions

    • Alexa Fluor® 488 Anti-TRAF2 antibody [EPR6048] (ab198971)
    • PE Anti-TRAF2 antibody [EPR6048] (ab213667)
    • Alexa Fluor® 594 Anti-TRAF2 antibody [EPR6048] (ab215491)
    • Anti-TRAF2 antibody [EPR6048] - BSA and Azide free (ab230795)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human TRAF2 knockout HEK-293T cell line (ab266060)
  • KO cell lysates

    • Human TRAF2 knockout HEK-293T cell lysate (ab257759)
  • KO cell pellets

    • Human TRAF2 knockout HEK-293T cell pellet (ab278906)
  • Recombinant Protein

    • Recombinant Human TRAF2 protein (ab204091)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab126758の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/120.

For unpurified, use 1/10 - 1/100.  ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 55 kDa).
IP
1/40.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

ICC/IF
1/100.
特記事項
Flow Cyt (Intra)
1/120.

For unpurified, use 1/10 - 1/100.  ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 55 kDa).
IP
1/40.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

ICC/IF
1/100.

ターゲット情報

  • 機能

    Regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis. Required for normal antibody isotype switching from IgM to IgG. Has E3 ubiquitin-protein ligase activity and promotes 'Lys-63'-linked ubiquitination of target proteins, such as BIRC3, RIPK1 and TICAM1. Is an essential constituent of several E3 ubiquitin-protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases. Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation; this does not depend on the TRAF2 RING-type zinc finger domain.
  • パスウェイ

    Protein modification; protein ubiquitination.
  • 配列類似性

    Belongs to the TNF receptor-associated factor family. A subfamily.
    Contains 1 MATH domain.
    Contains 1 RING-type zinc finger.
    Contains 2 TRAF-type zinc fingers.
  • ドメイン

    The coiled coil domain mediates homo- and hetero-oligomerization.
    The MATH/TRAF domain binds to receptor cytoplasmic domains.
    The RING-type zinc finger domain is essential for E3 ubiquitin-protein ligase activity. It is not essential for the stabilization of BIRC2, or for the ubiquitination of RIPK1 in response to TNFR1 signaling.
  • 翻訳後修飾

    Phosphorylated at several serine residues within the first 128 amino acid residues. Phosphorylated at Thr-117 in response to signaling via TNF and TNFRSF1A. Phosphorylation at Thr-117 is required for 'Lys-63'-linked polyubiquitination, but not for 'Lys-48'-linked polyubiquitination. Phosphorylation at Thr-117 is important for interaction with IKKA and IKKB, activation of IKK and subsequent activation of NF-kappa-B.
    Undergoes both 'Lys-48'-linked and 'Lys-63'-linked polyubiquitination. Polyubiquitinated via 'Lys-63'-linked ubiquitin in response to TNF signaling; this requires prior phosphorylation at Thr-117. 'Lys-63'-linked polyubiquitination promotes TRAF2-mediated activation of NF-kappa-B. Can be polyubiquitinated at several Lys residues via 'Lys-48'-linked ubiquitin chains in response to TNF signaling, leading to proteasomal degradation. Autoubiquitinated, leading to its subsequent proteasomal degradation. Polyubiquitinated by BIRC2 and SIAH2, leading to its subsequent proteasomal degradation. Deubiquitinated by CYLD, a protease that specifically cleaves 'Lys-63'-linked polyubiquitin chains.
  • 細胞内局在

    Cytoplasm.
  • Target information above from: UniProt accession Q12933 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 7186 Human
    • Entrez Gene: 22030 Mouse
    • Entrez Gene: 311786 Rat
    • Omim: 601895 Human
    • SwissProt: Q12933 Human
    • SwissProt: P39429 Mouse
    • Unigene: 522506 Human
    • Unigene: 3399 Mouse
    • 別名

      • E3 ubiquitin-protein ligase TRAF2 antibody
      • MGC:45012 antibody
      • OTTHUMP00000022625 antibody
      • OTTHUMP00000064745 antibody
      • TNF receptor associated factor 2 antibody
      • TNF receptor-associated factor 2 antibody
      • TNF receptor-associated protein antibody
      • TRAF 2 antibody
      • TRAF2 antibody
      • TRAF2_HUMAN antibody
      • TRAP 3 antibody
      • TRAP antibody
      • TRAP3 antibody
      • Tumor necrosis factor type 2 receptor associated protein 3 antibody
      • Tumor necrosis factor type 2 receptor-associated protein 3 antibody
      see all

    画像

    • Western blot - Anti-TRAF2 antibody [EPR6048] (ab126758)
      Western blot - Anti-TRAF2 antibody [EPR6048] (ab126758)
      All lanes : Anti-TRAF2 antibody [EPR6048] (ab126758) at 1/1000 dilution

      Lane 1 : Wild-type HEK-293T cell lysate
      Lane 2 : TRAF2 knockout HEK-293T cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 55 kDa
      Observed band size: 55 kDa



      Lanes 1- 2: Merged signal (red and green). Green - ab126758 observed at 55 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

       ab126758 was shown to react with TRAF2 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266060 (knockout cell lysate ab257759) was used. Wild-type HEK-293T and TRAF2 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab126758 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF2 antibody [EPR6048] (ab126758)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF2 antibody [EPR6048] (ab126758)
      Immunohistochemical staining of paraffin embedded human cervical cancer with purified ab126758 at a working dilution of 1/100. The secondary antibody used is ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Western blot - Anti-TRAF2 antibody [EPR6048] (ab126758)
      Western blot - Anti-TRAF2 antibody [EPR6048] (ab126758)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: TRAF2 knockout HAP1 cell lysate (20 µg)
      Lane 3: Human skeletal muscle lysate (20 µg)
      Lane 4: U2OS cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab126758 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab126758 was shown to specifically react with TRAF2 when TRAF2 knockout samples were used. Wild-type and TRAF2 knockout samples were subjected to SDS-PAGE. ab126758 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • Flow Cytometry (Intracellular) - Anti-TRAF2 antibody [EPR6048] (ab126758)
      Flow Cytometry (Intracellular) - Anti-TRAF2 antibody [EPR6048] (ab126758)

      Overlay histogram showing HeLa cells fixed in 2% PFA and stained with purified ab126758 at a dilution of 1 in 120 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 150. Rabbit monoclonal IgG was used as an isotype control (black) and cells without antibody were used as a negative control (blue).

       

    • Western blot - Anti-TRAF2 antibody [EPR6048] (ab126758)
      Western blot - Anti-TRAF2 antibody [EPR6048] (ab126758)
      All lanes : Anti-TRAF2 antibody [EPR6048] (ab126758) at 1/2000 dilution (purified)

      Lane 1 : Molt-4 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HEK293 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 55 kDa
      Observed band size: 53 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-TRAF2 antibody [EPR6048] (ab126758)
      Western blot - Anti-TRAF2 antibody [EPR6048] (ab126758)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-TRAF2 antibody [EPR6048] (ab126758)
      Western blot - Anti-TRAF2 antibody [EPR6048] (ab126758)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunocytochemistry/ Immunofluorescence - Anti-TRAF2 antibody [EPR6048] (ab126758)
      Immunocytochemistry/ Immunofluorescence - Anti-TRAF2 antibody [EPR6048] (ab126758)

      Immunofluorescence staining of HeLa cells with purified ab126758 at a working dilution of 1 in 100, counter-stained with DAPI. Tubulin was stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (ab150120) . The secondary antibody was ab150077 Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom middle and right hand panels - for the first negative control, purified ab126758 was used at a dilution of 1/200 followed by an Alexa Fluor® 555 goat anti-mouse antibody at a dilution of 1/500 and for the second negative control mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab15007) were used.

    • Immunoprecipitation - Anti-TRAF2 antibody [EPR6048] (ab126758)
      Immunoprecipitation - Anti-TRAF2 antibody [EPR6048] (ab126758)
      ab126758 (purified) at 1/40 immunoprecipitating TRAF2 in HEK293 (Lane 1 and 2). Lane 3 - PBS. For western blotting a HRP-conjugated anti-rabbit IgG specific to the non-reduced form of IgG was used as the secondary antibody (1/1500). Blocking buffer and concentration: 5% NFDM/TBST. Diluting buffer and concentration: 5% NFDM /TBST.
    • Flow Cytometry (Intracellular) - Anti-TRAF2 antibody [EPR6048] (ab126758)
      Flow Cytometry (Intracellular) - Anti-TRAF2 antibody [EPR6048] (ab126758)

      Overlay histogram showing HeLa cells stained with unpurifiedab126758 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (unpurified ab126758, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. 

       

       

    • Western blot - Anti-TRAF2 antibody [EPR6048] (ab126758)
      Western blot - Anti-TRAF2 antibody [EPR6048] (ab126758)
      All lanes : Anti-TRAF2 antibody [EPR6048] (ab126758) at 1/1000 dilution (unpurified)

      Lane 1 : PC12 cell lysate
      Lane 2 : MOLT4 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution

      Predicted band size: 55 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF2 antibody [EPR6048] (ab126758)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF2 antibody [EPR6048] (ab126758)

      Unpurified ab126758, at 1/50 dilution, staining TRAF2 in paraffin-embedded Human kidney tissue, by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-TRAF2 antibody [EPR6048] (ab126758)
      Immunocytochemistry/ Immunofluorescence - Anti-TRAF2 antibody [EPR6048] (ab126758)

      Unpurified ab126758, at 1/100 dilution, staining TRAF2 in HeLa cells, by Immunofluorescence.

    • OI-RD Scanning - Anti-TRAF2 antibody [EPR6048] (ab126758)
      OI-RD Scanning - Anti-TRAF2 antibody [EPR6048] (ab126758)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-TRAF2 antibody [EPR6048] (ab126758)
      Anti-TRAF2 antibody [EPR6048] (ab126758)

    プロトコール

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet download

      Download

    参考文献 (16)

    ab126758 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab126758 は 16 報の論文で使用されています。

    • Zhang Z  et al. HFD-induced TRAF6 upregulation promotes liver cholesterol accumulation and fatty liver development via EZH2-mediated miR-429/PPARa axis. Mol Ther Nucleic Acids 24:711-727 (2021). PubMed: 33996254
    • Liu J  et al. ERK signaling mediates resistance to immunomodulatory drugs in the bone marrow microenvironment. Sci Adv 7:N/A (2021). PubMed: 34088671
    • Li Z  et al. Changes in the circRNA expression profile of PC12 cells induced by TDCIPP exposure may regulate the downstream NF-?B pathway via the Traf2 gene. Chemosphere 254:126834 (2020). PubMed: 32339792
    • Liu Y  et al. Methamphetamine induces GSDME-dependent cell death in hippocampal neuronal cells through the endoplasmic reticulum stress pathway. Brain Res Bull 162:73-83 (2020). PubMed: 32544512
    • Zhai C  et al. Sphingosine-1-phosphate promotes pulmonary artery smooth muscle cells proliferation by stimulating autophagy-mediated E-cadherin/CDH1 down-regulation. Eur J Pharmacol 884:173302 (2020). PubMed: 32659302
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab126758.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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    製品・研究分野別
    • 癌
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    • 細胞生物学
    • 発生
    • エピジェネティクス&核内情報
    • 免疫学
    • メタボリズム
    • 感染症
    • ニューロサイエンス
    • シグナル伝達
    • ステムセル
    製品・タイプ別
    • 一次抗体
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