Anti-TRA-1-81 抗体 [TRA-1-81] - Stem Cell Marker (ab16289)
![Western blot - Anti-TRA-1-81 antibody [TRA-1-81] - Stem Cell Marker (ab16289)](https://www.abcam.co.jp/ps/products/16/ab16289/Images/ab16289-276571-anti-tra-1-81-antibody-tra-1-81-western-blot.jpg "Western blot - Anti-TRA-1-81 antibody [TRA-1-81] - Stem Cell Marker (ab16289)")
Key features and details
- Mouse monoclonal [TRA-1-81] to TRA-1-81 - Stem Cell Marker
- Suitable for: WB
- Reacts with: Human
- Isotype: IgM
製品の概要
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製品名
Anti-TRA-1-81 antibody [TRA-1-81] - Stem Cell Marker -
製品の詳細
Mouse monoclonal [TRA-1-81] to TRA-1-81 - Stem Cell Marker -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Rabbit
非交差種:
Mouse -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: HUES7 whole cell lysate.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
精製度
Tissue culture supernatant -
特記事項（精製）
Tissue culture supernatant was cross flow concentrated and buffer exchanged to PBS -
ポリ/モノ
モノクローナル -
クローン名
TRA-1-81 -
ミエローマ
P3x66Ag.8-Sp2/0 -
アイソタイプ
IgM -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab16289の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 200-400 kDa.
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| 特記事項 |
|---|
|
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 200-400 kDa. |
ターゲット情報
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関連性
The Podocalyxin gene encodes a member of the sialomucin protein family, and the encoded protein was originally identified as an important component of glomerular podocytes. Other biological activities of the encoded protein include: playing a role in hematopoetic cell differentiation, binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements and being expressed in vascular endothelium cells and binding to L-selectin. -
細胞内局在
Membrane; Single-pass type I membrane protein -
参照データベース
- Entrez Gene: 5420 Human
- Omim: 602632 Human
- SwissProt: O00592 Human
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別名
- Gp200 antibody
- PC antibody
- PCLP antibody
- PCLP1 antibody
画像
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Western blot - Anti-TRA-1-81 antibody [TRA-1-81] - Stem Cell Marker (ab16289)Anti-TRA-1-81 antibody [TRA-1-81] - Stem Cell Marker (ab16289) at 3 µg/ml + HUES7 (Human embryonic stem cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 200-400 kDa
Exposure time: 20 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab16289 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ab133406.
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![Western blot - Anti-TRA-1-81 antibody [TRA-1-81] - Stem Cell Marker (ab16289)](https://www.abcam.co.jp/ps/products/16/ab16289/Images/ab16289-24943-anti-tra-1-81-antibody-tra-1-81-western-blot.jpg)
Western blot - Anti-TRA-1-81 antibody [TRA-1-81] - Stem Cell Marker (ab16289)Anti-TRA-1-81 antibody [TRA-1-81] - Stem Cell Marker (ab16289) at 3 µg/ml + MEL-2 (Human embryonic stem cell, female cell line) Whole Cell Lysate (ab27196) at 10 µg
Secondary
Goat F(ab')2 Anti-Mouse IgM mu chain (HRP) (ab5930) at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 200-400 kDa
Exposure time: 3 minutes
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (37)
ab16289 は 37 報の論文で使用されています。
- Schrank S et al. Human-Induced Neurons from Presenilin 1 Mutant Patients Model Aspects of Alzheimer's Disease Pathology. Int J Mol Sci 21:N/A (2020). PubMed: 32033164
- Castro-Viñuelas R et al. Generation and characterization of human induced pluripotent stem cells (iPSCs) from hand osteoarthritis patient-derived fibroblasts. Sci Rep 10:4272 (2020). PubMed: 32144293
- Woods S et al. Generation of Human-Induced Pluripotent Stem Cells From Anterior Cruciate Ligament. J Orthop Res 38:92-104 (2020). PubMed: 31613026
- Castro-Viñuelas R et al. Generation of a human control iPS cell line (ESi080-A) from a donor with no rheumatic diseases. Stem Cell Res 43:101683 (2020). PubMed: 31962233
- Wang L et al. Establishment of a feeder and serum-free culture system for human embryonic stem cells. Zygote 28:175-182 (2020). PubMed: 31965957
