Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Embryonic Stem Cell, HUES7)
Specification
Embryonic Stem Cell, HUES7
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Other product details
Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: 1% serum, 0.1% Titon in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti mouse IgM
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Dilution
1/100
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Fiona Lewis
Verified customer
投稿 Aug 05 2009