Anti-TR4 抗体 [EPR1773(2)] (ab109301)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1773(2)] to TR4
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-TR4 antibody [EPR1773(2)]
TR4 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR1773(2)] to TR4 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details
適用なし: ICC/IF or IHC-P -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- PC3 cell lysate, 293T cell lysate; Human testis tissue
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR1773(2) -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
KO cell lines
-
KO cell lysates
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109301の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 67 kDa (predicted molecular weight: 65 kDa).
|
|
IP |
1/10 - 1/100.
|
特記事項 |
---|
WB
1/1000 - 1/10000. Detects a band of approximately 67 kDa (predicted molecular weight: 65 kDa). |
IP
1/10 - 1/100. |
ターゲット情報
-
機能
Orphan nuclear receptor that can act as a repressor or activator of transcription. An important repressor of nuclear recptor signaling pathways such as retinoic acid receptor, retinoid X, vitamin D3 receptor, thyroid hormone receptor and estrogen receptor pathways. May regulate gene expression during the late phase of spermatogenesis. Together with NR2C1, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription including that of GATA1. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Plays a fundamental role in early embryonic development and embryonic stem cells. Required for normal spermatogenesis and cerebellum development. Appears to be important for neurodevelopmentally regulated behavior (By similarity). Activates transcriptional activity of LHCG. Antagonist of PPARA-mediated transactivation. -
配列類似性
Belongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
発生段階
Transiently repressed during the meiotic phase of spermatogenesis. -
翻訳後修飾
Phosphorylation on Ser-19 and Ser-68 is an important regulator of NR2C2-mediated transcriptional activity. Phosphorylation on these residues recruits the corepressor, NRIP1, leading to transcripional repression, whereas the nonphosphorylated form preferentially recruits the coactivator, PCAF. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 7182 Human
- Entrez Gene: 22026 Mouse
- Entrez Gene: 50659 Rat
- Omim: 601426 Human
- SwissProt: P49116 Human
- SwissProt: P49117 Mouse
- SwissProt: P55094 Rat
- Unigene: 555973 Human
see all -
製品の状態
There are 2 isoforms produced by alternative splicing. -
別名
- hTAK1 antibody
- Nr2c2 antibody
- NR2C2_HUMAN antibody
see all
画像
-
All lanes : Anti-TR4 antibody [EPR1773(2)] (ab109301) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : NR2C2 CRISPR/Cas9 edited HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 65 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab109301 observed at 67 kDa. Red - loading control ab8245 observed at 36 kDa.
ab109301 Anti-TR4 antibody [EPR1773(2)] was shown to specifically react with TR4 in wild-type HEK293T cells. The band observed in CRISPR/Cas9 edited cell line ab266228 (CRISPR/Cas9 edited cell lysate ab257563) lane below 67 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and TR4 CRISPR/Cas9 edited samples were subjected to SDS-PAGE. ab109301 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
ab109301 (purified) at 1/30 immunoprecipitating TR4 in 10 μg HEK293 cell lysate (Lanes 1 and 2, observed at 67 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
-
All lanes : Anti-TR4 antibody [EPR1773(2)] (ab109301) at 1/5000 dilution (purified)
Lane 1 : PC-12 whole cell lysate
Lane 2 : 3T3-L1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
Anti-TR4 antibody [EPR1773(2)] (ab109301) at 1/5000 dilution (purified) + HEK-293 whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
Anti-TR4 antibody [EPR1773(2)] (ab109301) at 1/2000 dilution (purified) + PC-3 whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted? -
All lanes : Anti-TR4 antibody [EPR1773(2)] (ab109301) at 1/1000 dilution (Unpurified)
Lane 1 : PC3 cell lysate
Lane 2 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 65 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (9)
ab109301 は 9 報の論文で使用されています。
- Xia L et al. Targeting the TR4 nuclear receptor with antagonist bexarotene can suppress the proopiomelanocortin signalling in AtT-20 cells. J Cell Mol Med 25:2404-2417 (2021). PubMed: 33491272
- Wang H et al. Testicular Nuclear Receptor 4 Regulates Proliferation and Apoptosis of Bladder Cancer via Bcl-2. Front Mol Biosci 8:670409 (2021). PubMed: 34616769
- Vila-Cejudo M et al. Wnt pathway modulation generates blastomere-derived mouse embryonic stem cells with different pluripotency features. J Assist Reprod Genet 37:2967-2979 (2020). PubMed: 33047186
- Shi H et al. Targeting the TR4 nuclear receptor-mediated lncTASR/AXL signaling with tretinoin increases the sunitinib sensitivity to better suppress the RCC progression. Oncogene 39:530-545 (2020). PubMed: 31501521
- Hu L et al. Targeting TR4 nuclear receptor with antagonist bexarotene increases docetaxel sensitivity to better suppress the metastatic castration-resistant prostate cancer progression. Oncogene 39:1891-1903 (2020). PubMed: 31748715
- Chen Y et al. Testicular orphan receptor 4 promotes tumor progression and implies poor survival through AKT3 regulation in seminoma. Cancer Sci 109:384-394 (2018). PubMed: 29197138
- Wang M et al. Preclinical studies using miR-32-5p to suppress clear cell renal cell carcinoma metastasis via altering the miR-32-5p/TR4/HGF/Met signaling. Int J Cancer 143:100-112 (2018). PubMed: 29396852
- Al-Qahtani SM et al. 17ß-Estradiol suppresses visceral adipogenesis and activates brown adipose tissue-specific gene expression. Horm Mol Biol Clin Investig 29:13-26 (2017). PubMed: 27831918
- Zhang D et al. Targeting the ERK pathway for the treatment of Cushing's disease. Oncotarget 7:69149-69158 (2016). PubMed: 27708250