Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1772(2)] to TR4 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Human
製品名Anti-TR4 antibody [EPR1772(2)] - BSA and Azide free
TR4 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR1772(2)] to TR4 - BSA and Azide free
アプリケーション適用あり: IP, WBmore details
適用なし: Flow Cyt,ICC or IHC-P
Synthetic peptide within Human TR4 aa 1-100. The exact sequence is proprietary.
ab247887 is the carrier-free version of ab109513 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247887 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
Our Abpromise guarantee covers the use of ab247887 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 67 kDa (predicted molecular weight: 65 kDa).|
機能Orphan nuclear receptor that can act as a repressor or activator of transcription. An important repressor of nuclear recptor signaling pathways such as retinoic acid receptor, retinoid X, vitamin D3 receptor, thyroid hormone receptor and estrogen receptor pathways. May regulate gene expression during the late phase of spermatogenesis. Together with NR2C1, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription including that of GATA1. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Plays a fundamental role in early embryonic development and embryonic stem cells. Required for normal spermatogenesis and cerebellum development. Appears to be important for neurodevelopmentally regulated behavior (By similarity). Activates transcriptional activity of LHCG. Antagonist of PPARA-mediated transactivation.
配列類似性Belongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
発生段階Transiently repressed during the meiotic phase of spermatogenesis.
翻訳後修飾Phosphorylation on Ser-19 and Ser-68 is an important regulator of NR2C2-mediated transcriptional activity. Phosphorylation on these residues recruits the corepressor, NRIP1, leading to transcripional repression, whereas the nonphosphorylated form preferentially recruits the coactivator, PCAF.
- Information by UniProt
製品の状態There are 2 isoforms produced by alternative splicing.
- hTAK1 antibody
- Nr2c2 antibody
- NR2C2_HUMAN antibody
All lanes : Anti-TR4 antibody [EPR1772(2)] (ab109513) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : PC-3 cell lysate
Lane 3 : U937 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 65 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted?
This data was developed using ab109513, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.