リコンビナントRabMAb

Anti-TPX2 抗体 [EPR23180-4] - BSA and Azide free (ab270612)

Submit a review Submit a question
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPX2 antibody [EPR23180-4] - BSA and Azide free (ab270612)
  • Immunocytochemistry/ Immunofluorescence - Anti-TPX2 antibody [EPR23180-4] - BSA and Azide free (ab270612)
  • Immunoprecipitation - Anti-TPX2 antibody [EPR23180-4] - BSA and Azide free (ab270612)
  • Flow Cytometry - Anti-TPX2 antibody [EPR23180-4] - BSA and Azide free (ab270612)
  • Flow Cytometry - Anti-TPX2 antibody [EPR23180-4] - BSA and Azide free (ab270612)
  • Immunocytochemistry/ Immunofluorescence - Anti-TPX2 antibody [EPR23180-4] - BSA and Azide free (ab270612)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPX2 antibody [EPR23180-4] - BSA and Azide free (ab270612)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPX2 antibody [EPR23180-4] - BSA and Azide free (ab270612)
  • Anti-TPX2 antibody [EPR23180-4] - BSA and Azide free (ab270612)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23180-4] to TPX2 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-TPX2 antibody [EPR23180-4] - BSA and Azide free
    TPX2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23180-4] to TPX2 - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Raji, HEK-293 and HeLa whole cell lysate. IHC-P: Human lung cancer, breast cancer and colon cancer tissue. ICC/IF: HeLa and HEK-293 cells. Flow Cyt: HeLa and Raji cells. IP: Raji whole cell lysate.

  • 特記事項

    ab270612 is the carrier-free version of ab252945. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab270612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Human
IP
Human
アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 100 kDa (predicted molecular weight: 86 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 100 kDa (predicted molecular weight: 86 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Spindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation.

  • 組織特異性

    Expressed in lung carcinoma cell lines but not in normal lung tissues.

  • 配列類似性

    Belongs to the TPX2 family.

  • 発生段階

    Exclusively expressed in proliferating cells from the transition G1/S until the end of cytokinesis.

  • 翻訳後修飾

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • 細胞内局在

    Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus. Is released from the nucleus in apoptotic cells and is detected on apoptotic microtubules.
  • Information by UniProt

  • 参照データベース

    • 別名

      • C20ORF1 antibody
      • C20orf2 antibody
      • Chromosome 20 Open Reading Frame 1 antibody
      • Differentially expressed in cancerous and non-cancerous lung cells 2 antibody
      • Differentially expressed in cancerous and noncancerous lung cells 2 antibody
      • Differentially expressed in lung cells 2 antibody
      • Differentially expressed in lung cells antibody
      • DIL 2 antibody
      • DIL-2 antibody
      • DIL2 antibody
      • FLS353 antibody
      • GD:C20orf1 antibody
      • HCA 519 antibody
      • HCA519 antibody
      • HCTP 4 antibody
      • HCTP4 antibody
      • Hepatocellular carcinoma associated antigen 519 antibody
      • Hepatocellular carcinoma-associated antigen 519 antibody
      • p100 antibody
      • Preferentially expressed in colorectal cancer antibody
      • Protein fls353 antibody
      • REPP 86 antibody
      • repp86 antibody
      • Restricted expression proliferation associated protein 100 antibody
      • Restricted expression proliferation-associated protein 100 antibody
      • Targeting protein for Xklp2 antibody
      • TPX 2 antibody
      • TPX2 antibody
      • TPX2 microtubule associated homolog antibody
      • TPX2 microtubule associated protein homolog antibody
      • TPX2 microtubule nucleation factor antibody
      • TPX2_HUMAN antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPX2 antibody [EPR23180-4] - BSA and Azide free (ab270612)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPX2 antibody [EPR23180-4] - BSA and Azide free (ab270612)

      Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling TPX2 with ab252945 at 1/4000 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in proliferative cells of human colon cancer is observed(PMID:23634259, 22207630). The section was incubated with ab252945 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252945).

    • Immunocytochemistry/ Immunofluorescence - Anti-TPX2 antibody [EPR23180-4] - BSA and Azide free (ab270612)
      Immunocytochemistry/ Immunofluorescence - Anti-TPX2 antibody [EPR23180-4] - BSA and Azide free (ab270612)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling TPX2 with ab252945 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing positive staining in HeLa cells. ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252945).

    • Immunoprecipitation - Anti-TPX2 antibody [EPR23180-4] - BSA and Azide free (ab270612)
      Immunoprecipitation - Anti-TPX2 antibody [EPR23180-4] - BSA and Azide free (ab270612)

      TPX2 was immunoprecipitated from 0.35 mg Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate with ab252945 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab252945 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: Raji whole cell lysate 10 µg.

      Lane 2: ab252945 IP in Raji whole cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252945 in Raji whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 41 secs.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252945).

    • Flow Cytometry - Anti-TPX2 antibody [EPR23180-4] - BSA and Azide free (ab270612)
      Flow Cytometry - Anti-TPX2 antibody [EPR23180-4] - BSA and Azide free (ab270612)

      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling TPX2 with ab252945 at 1/500 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252945).

    • Flow Cytometry - Anti-TPX2 antibody [EPR23180-4] - BSA and Azide free (ab270612)
      Flow Cytometry - Anti-TPX2 antibody [EPR23180-4] - BSA and Azide free (ab270612)

      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling TPX2 with ab252945 at 1/500 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252945).

    • Immunocytochemistry/ Immunofluorescence - Anti-TPX2 antibody [EPR23180-4] - BSA and Azide free (ab270612)
      Immunocytochemistry/ Immunofluorescence - Anti-TPX2 antibody [EPR23180-4] - BSA and Azide free (ab270612)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293 cells labeling TPX2 with ab252945 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing positive staining in HEK-293 cells. ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252945).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPX2 antibody [EPR23180-4] - BSA and Azide free (ab270612)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPX2 antibody [EPR23180-4] - BSA and Azide free (ab270612)

      Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling TPX2 with ab252945 at 1/4000 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in proliferative cells of human breast cancer is observed (PMID:31494045). The section was incubated with ab252945 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252945).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPX2 antibody [EPR23180-4] - BSA and Azide free (ab270612)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPX2 antibody [EPR23180-4] - BSA and Azide free (ab270612)

      Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling TPX2 with ab252945 at 1/4000 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in proliferative cells of human lung cancer tissue is observed (PMID:23634259, 16489064). The section was incubated with ab252945 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252945).

    • Anti-TPX2 antibody [EPR23180-4] - BSA and Azide free (ab270612)
      Anti-TPX2 antibody [EPR23180-4] - BSA and Azide free (ab270612)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab270612 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab270612 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab270612.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com