Anti-TPT1L + TCTP 抗体 [EPR13882(B)] (ab182557)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13882(B)] to TPT1L + TCTP
- Suitable for: Flow Cyt (Intra), IHC-P, WB, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-TPT1L + TCTP antibody [EPR13882(B)]
TPT1L + TCTP 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR13882(B)] to TPT1L + TCTP -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), IHC-P, WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- Jurkat, 293 and HepG2 cell lysates; Human prostate tissue; Jurkat cells.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR13882(B) -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab182557の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/240.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
WB |
1/1000 - 1/2000. Detects a band of approximately 22 kDa (predicted molecular weight: 16, 20 kDa).
|
|
IP |
1/40 - 1/60.
|
特記事項 |
---|
Flow Cyt (Intra)
1/240. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000 - 1/2000. Detects a band of approximately 22 kDa (predicted molecular weight: 16, 20 kDa). |
IP
1/40 - 1/60. |
画像
-
All lanes : Anti-TPT1L + TCTP antibody [EPR13882(B)] (ab182557) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : 293 cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 16, 20 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling FLJ44635 + TCTP with ab182557 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling FLJ44635 + TCTP with ab182557 at 1/240 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
-
Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate immunoprecipitated using ab182557 at 1/50 dilution (Lane 1). Lane 2: Negative control.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
Certificate of Compliance
参考文献 (0)
ab182557 は論文での使用が確認できていません。